Restriction map for pF1KB5822
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AflII       AgeI        AhdI        AocI        
Aor51HI     ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AvrII       BamHI       
BbeI        BcgI        BclI        BfrI        BglII       
BlnI        BsaBI       BsiEI       BsiWI       BspDI       
BspHI       BspLU11I    BsrDI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
FbaI        FseI        HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MunI        MvnI        NarI        
NdeI        NheI        NotI        NruI        NspV        
PacI        PinAI       PmeI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfoI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspI        SwaI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccIII       1055  TCCGGA               T^CCGGA
Alw44I       1174  GTGCAC               G^TGCAC
ApaLI        1174  GTGCAC               G^TGCAC
AspI          703  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         221  TGCGCA               TGC^GCA
BbsI           67  GTCTTC               GAAGAC(2/6)
BbvCI        1291  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1145  GTATCC               GTATCC(6/5)
BfaI         1253  CTAG                 C^TAG
BlpI         1216  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1216  GCTGAGC              GC^T[ACGT]AGC
BpuAI          67  GTCTTC               GAAGAC(2/6)
BsaI          493  GAGACC               GGTCTC(1/5)
BsaWI        1055  TCCGGA               [AT]^CCGG[AT]
BseAI        1055  TCCGGA               T^CCGGA
BsmBI         936  GAGACG               CGTCTC(1/5)
BsmI          977  GAATGC               GAATGC(1/-1)
Bsp1407I      779  TGTACA               T^GTACA
BspEI        1055  TCCGGA               T^CCGGA
BsrBI         898  GAGCGG               CCGCTC(-3/-3)
BsrFI         189  GCCGGC               [GA]^CCGG[CT]
BsrGI         779  TGTACA               T^GTACA
BstYI        1494  GGATCT               [GA]^GATC[CT]
CelII        1216  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        189  GCCGGC               [GA]^CCGG[CT]
DrdI          790  GACCCCCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         888  CCTGGCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       292  ATGCAT               ATGCA^T
FspI          221  TGCGCA               TGC^GCA
HaeII         186  AGCGCC               [GA]GCGC^[CT]
MaeI         1253  CTAG                 C^TAG
MflI         1494  GGATCT               [GA]^GATC[CT]
MroI         1055  TCCGGA               T^CCGGA
MseI          925  TTAA                 T^TAA
NaeI          189  GCCGGC               GCC^GGC
NgoMIV        189  GCCGGC               G^CCGGC
NsiI          292  ATGCAT               ATGCA^T
PaeR7I       1169  CTCGAG               C^TCGAG
PflFI         703  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI         1519  GACTC                GAGTC(4/5)
PshAI        1420  GACGAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          451  CTGCAG               CTGCA^G
PvuII           7  CAGCTG               CAG^CTG
SacI          862  GAGCTC               GAGCT^C
SfcI          451  CTGCAG               C^T[GA][CT]AG
SfiI         1572  GGCCTCCAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         1431  CCCGGG               CCC^GGG
SspBI         779  TGTACA               T^GTACA
TaqI         1170  TCGA                 T^CGA
TfiI          432  GAATC                G^A[AT]TC
Tru9I         925  TTAA                 T^TAA
Tsp509I       927  AATT                 ^AATT
Tth111I       703  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1170  TCGA                 T^CGA
XhoI         1169  CTCGAG               C^TCGAG
XhoII        1494  GGATCT               [GA]^GATC[CT]
XmaCI        1431  CCCGGG               C^CCGGG
XmaI         1431  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcyI          204  GGCGTC               G[GA]^CG[CT]C
              817  GGCGTC              
AfaI          483  GTAC                 GT^AC
              780  GTAC                
AflIII        140  ACACGT               A^C[GA][CT]GT
              585  ACACGT              
ApaI          884  GGGCCC               GGGCC^C
             1263  GGGCCC              
BalI          632  TGGCCA               TGG^CCA
              890  TGGCCA              
BanI         1024  GGCACC               G^G[CT][GA]CC
             1452  GGCACC              
BbrPI         141  CACGTG               CAC^GTG
              586  CACGTG              
BglI          196  GCCACCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1573  GCCTCCAAGGC         
BsaAI         141  CACGTG               [CT]AC^GT[GA]
              586  CACGTG              
BsaHI         204  GGCGTC               G[GA]^CG[CT]C
              817  GGCGTC              
BsgI          640  CTGCAC               GTGCAG(16/14)
              415  GTGCAG              
BsrI           94  CCAGT                ACTGG(1/-1)
              378  CCAGT               
BstXI         995  CCAATGACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1085  CCAGGGAGCTGG        
DsaI          200  CCATGG               C^C[GA][CT]GG
              629  CCATGG              
EarI           44  CTCTTC               CTCTTC(1/4)
             1258  GAAGAG              
Hin1I         204  GGCGTC               G[GA]^CG[CT]C
              817  GGCGTC              
HinfI         432  GAATC                G^A[ACGT]TC
             1519  GACTC               
Ksp632I        44  CTCTTC               CTCTTC(1/4)
             1258  GAAGAG              
MluNI         632  TGGCCA               TGG^CCA
              890  TGGCCA              
MscI          632  TGGCCA               TGG^CCA
              890  TGGCCA              
NcoI          200  CCATGG               C^CATGG
              629  CCATGG              
PmaCI         141  CACGTG               CAC^GTG
              586  CACGTG              
PmlI          141  CACGTG               CAC^GTG
              586  CACGTG              
RsaI          483  GTAC                 GT^AC
              780  GTAC                
SmlI         1033  CTCAAG               C^T[CT][GA]AG
             1169  CTCGAG              
SphI          224  GCATGC               GCATG^C
              272  GCATGC              
StuI         1037  AGGCCT               AGG^CCT
             1571  AGGCCT              
XcmI          620  CCAATAGCCCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1487  CCAAGAAGGATCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AspHI       
AvaI        AvaII       BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BssKI       BstAPI      BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PpuMI       Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        TseI        
Tsp45I      TspRI       Van91I      
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