Restriction map for pF1KB5822
 (Linear) (Six-base) MAPSORT of: KIBB5822.seq  Check: 1504  from: 1  to: 1629



KIBB5822.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1221    1629 
   Size:       1221     408
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1371    1587    1629 
   Size:       1371     216      42

  Fragments arranged by size: 

                1371     216      42
 
AflIII A'CryG_T

Cuts at:      0     140     585    1629 
   Size:        140     445    1044

  Fragments arranged by size: 

                1044     445     140
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     235    1629 
   Size:        235    1394
 
AlwNI CAG_nnn'CTG

Cuts at:      0     123     833     874    1155    1248    1629 
   Size:        123     710      41     281      93     381

  Fragments arranged by size: 

                 710     381     281     123      93      41
 
ApaI G_GGCC'C

Cuts at:      0     888    1267    1629 
   Size:        888     379     362

  Fragments arranged by size: 

                 888     379     362
 
ApaLI G'TGCA_C

Cuts at:      0    1174    1629 
   Size:       1174     455
 
AvaI C'yCGr_G

Cuts at:      0    1169    1431    1567    1629 
   Size:       1169     262     136      62

  Fragments arranged by size: 

                1169     262     136      62
 
BanI G'GyrC_C

Cuts at:      0    1024    1452    1629 
   Size:       1024     428     177

  Fragments arranged by size: 

                1024     428     177
 
BanII G_rGCy'C

Cuts at:      0      62     541     866     888    1267    1629 
   Size:         62     479     325      22     379     362

  Fragments arranged by size: 

                 479     379     362     325      62      22
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      60    1629 
   Size:         60    1569
 
BbvCI CC'TCA_GC

Cuts at:      0    1292    1629 
   Size:       1292     337
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1156    1629 
   Size:       1156     473
 
BfrBI ATG'CAT

Cuts at:      0     294    1629 
   Size:        294    1335
 
BglI GCCn_nnn'nGGC

Cuts at:      0     202    1579    1629 
   Size:        202    1377      50

  Fragments arranged by size: 

                1377     202      50
 
BlpI GC'TnA_GC

Cuts at:      0    1217    1629 
   Size:       1217     412
 
Bme1580I G_kGCm'C

Cuts at:      0     888    1027    1178    1267    1629 
   Size:        888     139     151      89     362

  Fragments arranged by size: 

                 888     362     151     139      89
 
BmgBI CAC'GTC

Cuts at:      0    1008    1629 
   Size:       1008     621
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      88    1629 
   Size:         88    1541
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1119    1629 
   Size:       1119     510
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448    1114    1135    1177    1629 
   Size:        448     666      21      42     452

  Fragments arranged by size: 

                 666     452     448      42      21
 
Bpu10I CC'TnA_GC

Cuts at:      0    1292    1307    1399    1629 
   Size:       1292      15      92     230

  Fragments arranged by size: 

                1292     230      92      15
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1018    1629 
   Size:       1018     611
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     487    1629 
   Size:        487    1142
 
BsaAI yAC'GTr

Cuts at:      0     143     588    1629 
   Size:        143     445    1041

  Fragments arranged by size: 

                1041     445     143
 
BsaHI Gr'CG_yC

Cuts at:      0     205     818    1629 
   Size:        205     613     811

  Fragments arranged by size: 

                 811     613     205
 
BsaWI w'CCGG_w

Cuts at:      0    1055    1629 
   Size:       1055     574
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     127     157     472     502     634     664    1629 
   Size:        127      30     315      30     132      30     965

  Fragments arranged by size: 

                 965     315     132     127      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      63     148     271     588    1627    1629 
   Size:         63      85     123     317    1039       2

  Fragments arranged by size: 

                1039     317     123      85      63       2
 
BseYI C'CCAG_C

Cuts at:      0     528    1226    1284    1355    1477    1629 
   Size:        528     698      58      71     122     152

  Fragments arranged by size: 

                 698     528     152     122      71      58
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     625    1629 
   Size:        436     189    1004

  Fragments arranged by size: 

                1004     436     189
 
BsiHKAI G_wGCw'C

Cuts at:      0     618     866    1178    1629 
   Size:        618     248     312     451

  Fragments arranged by size: 

                 618     451     312     248
 
BsmI GAATG_Cn'

Cuts at:      0     983    1629 
   Size:        983     646
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     930    1629 
   Size:        930     699
 
Bsp1286I G_dGCh'C

Cuts at:      0      62     541     618     866     888    1027    1178    1267 
   Size:         62     479      77     248      22     139     151      89

Cuts at:   1267    1629 
   Size:        362

  Fragments arranged by size: 

                 479     362     248     151     139      89      77      62
                  22
 
BspEI T'CCGG_A

Cuts at:      0    1055    1629 
   Size:       1055     574
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     164    1082    1221    1629 
   Size:        164     918     139     408

  Fragments arranged by size: 

                 918     408     164     139
 
BsrBI CCG'CTC

Cuts at:      0     900    1629 
   Size:        900     729
 
BsrFI r'CCGG_y

Cuts at:      0     189    1629 
   Size:        189    1440
 
BsrGI T'GTAC_A

Cuts at:      0     779    1629 
   Size:        779     850
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     234     300     833    1248    1629 
   Size:        234      66     533     415     381

  Fragments arranged by size: 

                 533     415     381     234      66
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1002    1092    1629 
   Size:       1002      90     537

  Fragments arranged by size: 

                1002     537      90
 
BstYI r'GATC_y

Cuts at:      0    1494    1629 
   Size:       1494     135
 
BtgI C'CryG_G

Cuts at:      0     200     629    1629 
   Size:        200     429    1000

  Fragments arranged by size: 

                1000     429     200
 
BtsI GCAGTG_nn'

Cuts at:      0    1312    1522    1629 
   Size:       1312     210     107

  Fragments arranged by size: 

                1312     210     107
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     796    1629 
   Size:        796     833
 
EaeI y'GGCC_r

Cuts at:      0     353     632     890    1629 
   Size:        353     279     258     739

  Fragments arranged by size: 

                 739     353     279     258
 
EarI CTCTTCn'nnn_

Cuts at:      0      50    1253    1629 
   Size:         50    1203     376

  Fragments arranged by size: 

                1203     376      50
 
EcoICRI GAG'CTC

Cuts at:      0     864    1629 
   Size:        864     765
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448    1114    1135    1177    1371    1587    1629 
   Size:        448     666      21      42     194     216      42

  Fragments arranged by size: 

                 666     448     216     194      42      42      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     892    1629 
   Size:        892     737
 
EcoO109I rG'GnC_Cy

Cuts at:      0     884     885    1071    1263    1275    1296    1426    1629 
   Size:        884       1     186     192      12      21     130     203

  Fragments arranged by size: 

                 884     203     192     186     130      21      12       1
            
 
FspI TGC'GCA

Cuts at:      0     223    1629 
   Size:        223    1406
 
HaeII r_GCGC'y

Cuts at:      0     190    1629 
   Size:        190    1439
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     472     504    1152    1184    1629 
   Size:        472      32     648      32     445

  Fragments arranged by size: 

                 648     472     445      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     650     677    1629 
   Size:        650      27     952

  Fragments arranged by size: 

                 952     650      27
 
MscI TGG'CCA

Cuts at:      0     634     892    1629 
   Size:        634     258     737

  Fragments arranged by size: 

                 737     634     258
 
MslI CAynn'nnrTG

Cuts at:      0     634     773    1210    1629 
   Size:        634     139     437     419

  Fragments arranged by size: 

                 634     437     419     139
 
MspA1I CmG'CkG

Cuts at:      0       9     264     604    1341    1533    1629 
   Size:          9     255     340     737     192      96

  Fragments arranged by size: 

                 737     340     255     192      96       9
 
NaeI GCC'GGC

Cuts at:      0     191    1629 
   Size:        191    1438
 
NcoI C'CATG_G

Cuts at:      0     200     629    1629 
   Size:        200     429    1000

  Fragments arranged by size: 

                1000     429     200
 
NgoMIV G'CCGG_C

Cuts at:      0     189    1629 
   Size:        189    1440
 
NsiI A_TGCA'T

Cuts at:      0     296    1629 
   Size:        296    1333
 
NspI r_CATG'y

Cuts at:      0     228     276     303    1629 
   Size:        228      48      27    1326

  Fragments arranged by size: 

                1326     228      48      27
 
PasI CC'CwG_GG

Cuts at:      0     534     568    1267    1629 
   Size:        534      34     699     362

  Fragments arranged by size: 

                 699     534     362      34
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1155    1233    1380    1629 
   Size:       1155      78     147     249

  Fragments arranged by size: 

                1155     249     147      78
 
PfoI T'CCnGG_A

Cuts at:      0    1148    1511    1629 
   Size:       1148     363     118

  Fragments arranged by size: 

                1148     363     118
 
PmlI CAC'GTG

Cuts at:      0     143     588    1629 
   Size:        143     445    1041

  Fragments arranged by size: 

                1041     445     143
 
PpuMI rG'GwC_Cy

Cuts at:      0    1071    1296    1426    1629 
   Size:       1071     225     130     203

  Fragments arranged by size: 

                1071     225     203     130
 
PshAI GACnn'nnGTC

Cuts at:      0    1424    1629 
   Size:       1424     205
 
PspOMI G'GGCC_C

Cuts at:      0     884    1263    1629 
   Size:        884     379     366

  Fragments arranged by size: 

                 884     379     366
 
PspXI vC'TCGA_Gb

Cuts at:      0    1169    1629 
   Size:       1169     460
 
PstI C_TGCA'G

Cuts at:      0     455    1629 
   Size:        455    1174
 
PvuII CAG'CTG

Cuts at:      0       9    1629 
   Size:          9    1620
 
SacI G_AGCT'C

Cuts at:      0     866    1629 
   Size:        866     763
 
SanDI GG'GwC_CC

Cuts at:      0    1426    1629 
   Size:       1426     203
 
SfcI C'TryA_G

Cuts at:      0     451    1629 
   Size:        451    1178
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1579    1629 
   Size:       1579      50
 
SmaI CCC'GGG

Cuts at:      0    1433    1629 
   Size:       1433     196
 
SmlI C'TyrA_G

Cuts at:      0    1033    1169    1629 
   Size:       1033     136     460

  Fragments arranged by size: 

                1033     460     136
 
SphI G_CATG'C

Cuts at:      0     228     276    1629 
   Size:        228      48    1353

  Fragments arranged by size: 

                1353     228      48
 
StuI AGG'CCT

Cuts at:      0    1039    1573    1629 
   Size:       1039     534      56

  Fragments arranged by size: 

                1039     534      56
 
StyI C'CwwG_G

Cuts at:      0      32     200     629     711     893     989    1577    1629 
   Size:         32     168     429      82     182      96     588      52

  Fragments arranged by size: 

                 588     429     182     168      96      82      52      32
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1369    1629 
   Size:       1369     260
 
TatI w'GTAC_w

Cuts at:      0     779    1629 
   Size:        779     850
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     127     159     577     609     634     666    1629 
   Size:        127      32     418      32      25      32     963

  Fragments arranged by size: 

                 963     418     127      32      32      32      25
 
Tth111I GACn'n_nGTC

Cuts at:      0     706    1629 
   Size:        706     923
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     627    1494    1629 
   Size:        627     867     135

  Fragments arranged by size: 

                 867     627     135
 
XhoI C'TCGA_G

Cuts at:      0    1169    1629 
   Size:       1169     460
 
XmaI C'CCGG_G

Cuts at:      0    1431    1629 
   Size:       1431     198
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AlfI    AlwNI     ApaI    ApaLI     AvaI
     BanI    BanII     BbsI    BbvCI    BciVI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BsrGI
   BstAPI    BstXI    BstYI     BtgI     BtsI     DrdI     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NsiI     NspI
     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI    PspXI
     PstI    PvuII     SacI    SanDI     SfcI     SfiI     SmaI     SmlI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BcgI     BclI    BglII     BmtI    BsaBI    BsiEI
    BsiWI    BspHI    BsrDI   BssHII    BssSI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     EagI     EciI
    EcoRI    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NarI     NdeI     NheI     NotI
     NruI     PacI     PciI     PmeI     PpiI     PsiI     PsrI     PvuI
    RsrII    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI
     XmnI     ZraI
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