Restriction map for pF1KB5817
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BanI        BbeI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HgaI        Hin1I       
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MamI        MfeI        MluI        MroI        
MspA1I      MunI        MvaI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SapI        Sau96I      SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    StuI        
SwaI        TfiI        Tsp45I      Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   66 enzymes) #
###################################

AciI           79  GCGG                 CCGC(-3/-1)
AcsI          353  GAATTT               [GA]^AATT[CT]
AfaI          465  GTAC                 GT^AC
AflIII        413  ACACGT               A^C[GA][CT]GT
ApoI          353  GAATTT               [GA]^AATT[CT]
AspHI         629  GAGCTC               G[AT]GC[AT]^C
BalI          497  TGGCCA               TGG^CCA
BamHI         522  GGATCC               G^GATCC
BanII         629  GAGCTC               G[GA]GC[CT]^C
BbrPI         414  CACGTG               CAC^GTG
BbsI          336  GTCTTC               GAAGAC(2/6)
BcnI          518  CCGGG                CC^[GC]GG
BmyI          629  GAGCTC               G[AGT]GC[ACT]^C
BpuAI         336  GTCTTC               GAAGAC(2/6)
BsaAI         414  CACGTG               [CT]AC^GT[GA]
BseRI         385  GAGGAG               GAGGAG(10/8)
BsgI          559  CTGCAC               GTGCAG(16/14)
BsiHKAI       629  GAGCTC               G[AT]GC[AT]^C
BsmAI         616  GAGAC                GTCTC(1/5)
Bsp1286I      629  GAGCTC               G[AGT]GC[ACT]^C
Bsp1407I      464  TGTACA               T^GTACA
BspMI         127  GCAGGT               ACCTGC(4/8)
BsrGI         464  TGTACA               T^GTACA
BsrI          261  ACTGG                ACTGG(1/-1)
BssKI         518  CCGGG                ^CC[ACGT]GG
BstF5I        297  GGATG                GGATG(2/0)
BstYI         522  GGATCC               [GA]^GATC[CT]
CfoI          489  GCGC                 GCG^C
DdeI          538  CTGAG                C^T[ACGT]AG
DpnII         523  GATC                 ^GATC
EaeI          497  TGGCCA               [CT]^GGCC[GA]
FokI          297  GGATG                GGATG(9/13)
HapII         518  CCGG                 C^CGG
HhaI          489  GCGC                 GCG^C
HinP1I        489  GCGC                 G^CGC
HincII        303  GTTGAC               GT[CT]^[GA]AC
HindII        303  GTTGAC               GT[CT]^[GA]AC
HinfI          12  GACTC                G^A[ACGT]TC
HpaII         518  CCGG                 C^CGG
MaeII         415  ACGT                 A^CGT
MaeIII         25  GTAAC                ^GT[ACGT]AC
MboI          523  GATC                 ^GATC
MflI          522  GGATCC               [GA]^GATC[CT]
MluNI         497  TGGCCA               TGG^CCA
MscI          497  TGGCCA               TGG^CCA
MslI          430  CATTTCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          518  CCGG                 C^CGG
NciI          518  CCGGG                CC^[GC]GG
NdeII         523  GATC                 ^GATC
NlaIV         522  GGATCC               GG[ACGT]^[ACGT]CC
NspI          605  GCATGC               [GA]CATG^[CT]
PleI           12  GACTC                GAGTC(4/5)
PmaCI         414  CACGTG               CAC^GTG
PmlI          414  CACGTG               CAC^GTG
PstI          283  CTGCAG               CTGCA^G
RsaI          465  GTAC                 GT^AC
SacI          629  GAGCTC               GAGCT^C
Sau3AI        523  GATC                 ^GATC
ScrFI         518  CCGGG                CC^[ACGT]GG
SfcI          283  CTGCAG               C^T[GA][CT]AG
SphI          605  GCATGC               GCATG^C
SspBI         464  TGTACA               T^GTACA
SspI          148  AATATT               AAT^ATT
TaqI          217  TCGA                 T^CGA
TthHB8I       217  TCGA                 T^CGA
XhoII         522  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AlwI          523  GATCC                GGATC(4/5)
              522  GGATC               
BfaI          196  CTAG                 C^TAG
              364  CTAG                
HphI          318  GGTGA                GGTGA(8/7)
              533  TCACC               
MaeI          196  CTAG                 C^TAG
              364  CTAG                
MboII         229  GAAGA                GAAGA(8/7)
              337  TCTTC               
MseI           68  TTAA                 T^TAA
               97  TTAA                
MwoI          480  GCTCCTGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              558  GCTGCACCAGC         
NlaIII        552  CATG                 CATG^
              606  CATG                
SfaNI         124  GATGC                GCATC(5/9)
              594  GCATC               
Tru9I          68  TTAA                 T^TAA
               97  TTAA                
TspRI          54  CAGTG                CA[GC]TG[ACGT][ACGT]^
              138  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AluI        BbvI        BpmI        BsaJI       BsiYI       
BslI        Cac8I       EcoT14I     Fnu4HI      HaeIII      
ItaI        MnlI        StyI        TseI        Tsp509I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com