Restriction map for pF1KB7970
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AcyI        
AflII       AgeI        AhdI        AocI        Aor51HI     
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AvaI        AvrII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       FbaI        FseI        HaeII       HgaI        
Hin1I       HindIII     HpaI        KasI        KspI        
MamI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       PstI        
PvuI        PvuII       RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        Tth111I     Van91I      
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

Acc65I        438  GGTACC               G^GTACC
AclI          812  AACGTT               AA^CGTT
AflIII        426  ACATGT               A^C[GA][CT]GT
Alw44I        135  GTGCAC               G^TGCAC
AlwNI         833  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1175  GGGCCC               GGGCC^C
ApaLI         135  GTGCAC               G^TGCAC
Asp718I       438  GGTACC               G^GTACC
AvaII         805  GGTCC                G^G[AT]CC
AviII         158  TGCGCA               TGC^GCA
BalI          957  TGGCCA               TGG^CCA
BamHI         600  GGATCC               G^GATCC
BanI          438  GGTACC               G^G[CT][GA]CC
BbsI          761  GAAGAC               GAAGAC(2/6)
BpuAI         761  GAAGAC               GAAGAC(2/6)
BseRI         699  CTCCTC               GAGGAG(10/8)
BsmAI          81  GTCTC                GTCTC(1/5)
BsmFI         806  GTCCC                GGGAC(10/14)
BspLU11I      426  ACATGT               A^CATGT
BstXI        1190  CCAGGAAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         600  GGATCC               [GA]^GATC[CT]
Cac8I        1173  GCGGGC               GC[ACGT]^[ACGT]GC
CfoI          159  GCGC                 GCG^C
DpnII         601  GATC                 ^GATC
DraI           43  TTTAAA               TTT^AAA
DraII        1175  GGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI         1015  CCATGG               C^C[GA][CT]GG
EaeI          957  TGGCCA               [CT]^GGCC[GA]
EarI          401  GAAGAG               CTCTTC(1/4)
EcoNI          41  CCTTTAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1175  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT22I      1041  ATGCAT               ATGCA^T
FauI         1173  GCGGG                CCCGC(4/6)
FspI          158  TGCGCA               TGC^GCA
HhaI          159  GCGC                 GCG^C
HinP1I        159  GCGC                 G^CGC
HincII        245  GTCAAC               GT[CT]^[GA]AC
HindII        245  GTCAAC               GT[CT]^[GA]AC
KpnI          438  GGTACC               GGTAC^C
Ksp632I       401  GAAGAG               CTCTTC(1/4)
MboI          601  GATC                 ^GATC
MflI          600  GGATCC               [GA]^GATC[CT]
MluNI         957  TGGCCA               TGG^CCA
MscI          957  TGGCCA               TGG^CCA
MslI         1016  CATGGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1171  CAGCGG               C[AC]G^C[GT]G
NcoI         1015  CCATGG               C^CATGG
NdeII         601  GATC                 ^GATC
NsiI         1041  ATGCAT               ATGCA^T
PshAI        1162  GACTGTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      812  AACGTT               AA^CGTT
SacI          607  GAGCTC               GAGCT^C
SapI          401  GAAGAGC              GCTCTTC(1/4)
Sau3AI        601  GATC                 ^GATC
SmlI          443  CTCAAG               C^T[CT][GA]AG
TaqI          657  TCGA                 T^CGA
TthHB8I       657  TCGA                 T^CGA
XbaI          618  TCTAGA               T^CTAGA
XcmI         1141  CCATCAGGTCGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         600  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AciI          558  GCGG                 CCGC(-3/-1)
             1173  GCGG                
AcsI          361  GAATTT               [GA]^AATT[CT]
              878  GAATTT              
AlwI          601  GATCC                GGATC(4/5)
              600  GGATC               
ApoI          361  GAATTT               [GA]^AATT[CT]
              878  GAATTT              
AspHI         135  GTGCAC               G[AT]GC[AT]^C
              607  GAGCTC              
BcnI          381  CCGGG                CC^[GC]GG
              511  CCCGG               
BfaI          142  CTAG                 C^TAG
              619  CTAG                
BsiHKAI       135  GTGCAC               G[AT]GC[AT]^C
              607  GAGCTC              
BsiYI          41  CCTTTAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1010  CCTACCCATGG         
BslI           41  CCTTTAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1010  CCTACCCATGG         
BsrDI         155  CATTGC               GCAATG(2/0)
              707  GCAATG              
BsrI          314  CCAGT                ACTGG(1/-1)
              496  CCAGT               
BstF5I        507  CATCC                GGATG(2/0)
              961  CATCC               
EcoT14I      1015  CCATGG               C^C[AT][AT]GG
             1128  CCTTGG              
FokI          507  CATCC                GGATG(9/13)
              961  CATCC               
HapII         381  CCGG                 C^CGG
              512  CCGG                
HpaII         381  CCGG                 C^CGG
              512  CCGG                
MaeI          142  CTAG                 C^TAG
              619  CTAG                
MaeII         243  ACGT                 A^CGT
              813  ACGT                
MspI          381  CCGG                 C^CGG
              512  CCGG                
NciI          381  CCGGG                CC^[GC]GG
              511  CCCGG               
PleI          748  GACTC                GAGTC(4/5)
              898  GAGTC               
SspI           23  AATATT               AAT^ATT
              527  AATATT              
StyI         1015  CCATGG               C^C[AT][AT]GG
             1128  CCTTGG              
TfiI          634  GATTC                G^A[AT]TC
              922  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AfaI        AluI        BanII       BbvI        BmyI        
BsaJI       Bsp1286I    BssKI       BstNI       Cfr13I      
DdeI        EcoRII      Fnu4HI      HaeIII      HinfI       
HphI        ItaI        MaeIII      MboII       MnlI        
MseI        MvaI        MwoI        NlaIII      NlaIV       
NspI        RsaI        Sau96I      ScrFI       Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       
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