Restriction map for pF1KB7970
 (Linear) (Six-base) MAPSORT of: KIBB7970.seq  Check: 9757  from: 1  to: 1230



KIBB7970.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

Acc65I G'GTAC_C

Cuts at:      0     438    1230 
   Size:        438     792
 
AclI AA'CG_TT

Cuts at:      0     813    1230 
   Size:        813     417
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     737     907    1230 
   Size:        737     170     323

  Fragments arranged by size: 

                 737     323     170
 
AflIII A'CryG_T

Cuts at:      0     426    1230 
   Size:        426     804
 
AlwNI CAG_nnn'CTG

Cuts at:      0     838    1230 
   Size:        838     392
 
ApaI G_GGCC'C

Cuts at:      0    1179    1230 
   Size:       1179      51
 
ApaLI G'TGCA_C

Cuts at:      0     135    1230 
   Size:        135    1095
 
ApoI r'AATT_y

Cuts at:      0     361     878    1230 
   Size:        361     517     352

  Fragments arranged by size: 

                 517     361     352
 
BamHI G'GATC_C

Cuts at:      0     600    1230 
   Size:        600     630
 
BanI G'GyrC_C

Cuts at:      0     438    1230 
   Size:        438     792
 
BanII G_rGCy'C

Cuts at:      0     593     611    1179    1230 
   Size:        593      18     568      51

  Fragments arranged by size: 

                 593     568      51      18
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     768    1230 
   Size:        768     462
 
BfrBI ATG'CAT

Cuts at:      0    1043    1230 
   Size:       1043     187
 
Bme1580I G_kGCm'C

Cuts at:      0     139    1179    1230 
   Size:        139    1040      51

  Fragments arranged by size: 

                1040     139      51
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     490    1230 
   Size:        490     740
 
Bpu10I CC'TnA_GC

Cuts at:      0     605    1230 
   Size:        605     625
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     428    1230 
   Size:        428     802
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     690    1230 
   Size:        690     540
 
BsiHKAI G_wGCw'C

Cuts at:      0     139     611    1230 
   Size:        139     472     619

  Fragments arranged by size: 

                 619     472     139
 
Bsp1286I G_dGCh'C

Cuts at:      0     139     593     611    1179    1230 
   Size:        139     454      18     568      51

  Fragments arranged by size: 

                 568     454     139      51      18
 
BsrDI GCAATG_nn'

Cuts at:      0     154     714    1230 
   Size:        154     560     516

  Fragments arranged by size: 

                 560     516     154
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1197    1230 
   Size:       1197      33
 
BstYI r'GATC_y

Cuts at:      0     600    1230 
   Size:        600     630
 
BtgI C'CryG_G

Cuts at:      0    1015    1230 
   Size:       1015     215
 
BtsI GCAGTG_nn'

Cuts at:      0     168     933    1027    1230 
   Size:        168     765      94     203

  Fragments arranged by size: 

                 765     203     168      94
 
DraI TTT'AAA

Cuts at:      0      45    1230 
   Size:         45    1185
 
EaeI y'GGCC_r

Cuts at:      0     957    1230 
   Size:        957     273
 
EarI CTCTTCn'nnn_

Cuts at:      0     396    1230 
   Size:        396     834
 
EcoICRI GAG'CTC

Cuts at:      0     609    1230 
   Size:        609     621
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     737     907    1230 
   Size:        737     170     323

  Fragments arranged by size: 

                 737     323     170
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      45    1230 
   Size:         45    1185
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1176    1230 
   Size:       1176      54
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     422    1230 
   Size:        422     808
 
FspI TGC'GCA

Cuts at:      0     160    1230 
   Size:        160    1070
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     685     717    1230 
   Size:        685      32     513

  Fragments arranged by size: 

                 685     513      32
 
HincII GTy'rAC

Cuts at:      0     247    1230 
   Size:        247     983
 
KpnI G_GTAC'C

Cuts at:      0     442    1230 
   Size:        442     788
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     926    1230 
   Size:        926     304
 
MscI TGG'CCA

Cuts at:      0     959    1230 
   Size:        959     271
 
MslI CAynn'nnrTG

Cuts at:      0    1020    1230 
   Size:       1020     210
 
MspA1I CmG'CkG

Cuts at:      0    1173    1230 
   Size:       1173      57
 
NcoI C'CATG_G

Cuts at:      0    1015    1230 
   Size:       1015     215
 
NsiI A_TGCA'T

Cuts at:      0    1045    1230 
   Size:       1045     185
 
NspI r_CATG'y

Cuts at:      0     211     430    1043    1230 
   Size:        211     219     613     187

  Fragments arranged by size: 

                 613     219     211     187
 
PciI A'CATG_T

Cuts at:      0     426    1230 
   Size:        426     804
 
PfoI T'CCnGG_A

Cuts at:      0     521    1230 
   Size:        521     709
 
PshAI GACnn'nnGTC

Cuts at:      0    1166    1230 
   Size:       1166      64
 
PsiI TTA'TAA

Cuts at:      0     266     533    1230 
   Size:        266     267     697

  Fragments arranged by size: 

                 697     267     266
 
PspOMI G'GGCC_C

Cuts at:      0    1175    1230 
   Size:       1175      55
 
SacI G_AGCT'C

Cuts at:      0     611    1230 
   Size:        611     619
 
SapI GCTCTTCn'nnn_

Cuts at:      0     396    1230 
   Size:        396     834
 
SmlI C'TyrA_G

Cuts at:      0     443    1230 
   Size:        443     787
 
SspI AAT'ATT

Cuts at:      0      25     529    1230 
   Size:         25     504     701

  Fragments arranged by size: 

                 701     504      25
 
StyI C'CwwG_G

Cuts at:      0    1015    1128    1230 
   Size:       1015     113     102

  Fragments arranged by size: 

                1015     113     102
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      19    1203    1230 
   Size:         19    1184      27

  Fragments arranged by size: 

                1184      27      19
 
TatI w'GTAC_w

Cuts at:      0     452     819    1230 
   Size:        452     367     411

  Fragments arranged by size: 

                 452     411     367
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     416     448    1230 
   Size:        416      32     782

  Fragments arranged by size: 

                 782     416      32
 
XbaI T'CTAG_A

Cuts at:      0     618    1230 
   Size:        618     612
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1148    1230 
   Size:       1148      82
 

 Enzymes that do cut:

   Acc65I     AclI     AcuI   AflIII    AlwNI     ApaI    ApaLI     ApoI
    BamHI     BanI    BanII     BbsI    BfrBI Bme1580I     BmrI   Bpu10I
    BpuEI    BseRI  BsiHKAI Bsp1286I    BsrDI    BstXI    BstYI     BtgI
     BtsI     DraI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI     FspI    Hin4I   HincII     KpnI     MmeI     MscI     MslI
   MspA1I     NcoI     NsiI     NspI     PciI     PfoI    PshAI     PsiI
   PspOMI     SacI     SapI     SmlI     SspI     StyI    TaqII     TatI
     TstI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BbeI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
   DraIII     DrdI     EagI     EciI    EcoRI    EcoRV     FseI    FspAI
    HaeII  HindIII     HpaI     KasI     MfeI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI  Tth111I     XhoI     XmaI     XmnI
     ZraI
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