Restriction map for pF1KB7966
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       BalI        BamHI       BbrPI       
BbsI        BbvCI       BciVI       BclI        BfrI        
BglII       BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BseAI       BsiWI       BsmBI       BsmI        
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DrdI        
Eam1105I    EarI        Eco47III    Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
HphI        KpnI        Ksp632I     MaeII       MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SapI        SbfI        
ScaI        SexAI       SfuI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        TaqI        Tru9I       Tth111I     TthHB8I     
Van91I      XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          618  GTCTAC               GT^[AC][GT]AC
AlwNI         413  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          307  GGGCCC               GGGCC^C
AvrII         840  CCTAGG               C^CTAGG
BanI          377  GGCGCC               G^G[CT][GA]CC
BbeI          377  GGCGCC               GGCGC^C
BcgI          528  GCAGATTCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          309  GCCCCGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          840  CCTAGG               C^CTAGG
BsaI          799  GAGACC               GGTCTC(1/5)
BsaWI        1107  ACCGGA               [AT]^CCGG[AT]
BsgI          526  GTGCAG               GTGCAG(16/14)
Bsp1407I      817  TGTACA               T^GTACA
BsrGI         817  TGTACA               T^GTACA
BssHII        256  GCGCGC               G^CGCGC
BstXI         184  CCAGCGCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         696  AGATCC               [GA]^GATC[CT]
DraIII       1070  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          316  CGGCCG               C^GGCCG
EclXI         316  CGGCCG               C^GGCCG
Eco52I        316  CGGCCG               C^GGCCG
HgaI          142  GCGTC                GACGC(5/10)
KasI          377  GGCGCC               G^GCGCC
MflI          696  AGATCC               [GA]^GATC[CT]
NaeI          314  GCCGGC               GCC^GGC
NarI          377  GGCGCC               GG^CGCC
NcoI           85  CCATGG               C^CATGG
NgoMIV        314  GCCGGC               G^CCGGC
NspI          674  ACATGC               [GA]CATG^[CT]
PpuMI         226  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI         1169  CTGCAG               CTGCA^G
PvuII         821  CAGCTG               CAG^CTG
SacI          747  GAGCTC               GAGCT^C
SfiI          308  GGCCCCGCCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          377  GGCGCC               GGC^GCC
SgrAI         251  CACCGGCG             C[GA]^CCGG[CT]G
SspBI         817  TGTACA               T^GTACA
Tsp45I        919  GTGAC                ^GT[GC]AC
Tsp509I       714  AATT                 ^AATT
XbaI          792  TCTAGA               T^CTAGA
XhoII         696  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcyI          141  GGCGTC               G[GA]^CG[CT]C
              377  GGCGCC              
AlwI          697  GATCC                GGATC(4/5)
              877  GGATC               
AvaI          103  CCCGGG               C^[CT]CG[GA]G
              873  CCCGGG              
BsaBI         697  GATCCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              765  GATGGGCATC          
BsaHI         141  GGCGTC               G[GA]^CG[CT]C
              377  GGCGCC              
BsiEI         316  CGGCCG               CG[GA][CT]^CG
              959  CGGTCG              
BsoBI         103  CCCGGG               C^[CT]CG[GA]G
              873  CCCGGG              
BsrFI         252  ACCGGC               [GA]^CCGG[CT]
              314  GCCGGC              
Cfr10I        252  ACCGGC               [GA]^CCGG[CT]
              314  GCCGGC              
DraII         226  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              307  GGGCCCC             
EaeI          316  CGGCCG               [CT]^GGCC[GA]
              998  CGGCCA              
EcoNI         510  CCTACCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1113  CCTCAACCAGG         
EcoO109I      226  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              307  GGGCCCC             
HaeII         186  AGCGCC               [GA]GCGC^[CT]
              377  GGCGCC              
Hin1I         141  GGCGTC               G[GA]^CG[CT]C
              377  GGCGCC              
KspI          159  CCGCGG               CCGC^GG
              337  CCGCGG              
MamI          697  GATCCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              765  GATGGGCATC          
MboII         469  TCTTC                GAAGA(8/7)
              534  TCTTC               
SacII         159  CCGCGG               CCGC^GG
              337  CCGCGG              
SfcI          625  CTACAG               C^T[GA][CT]AG
             1169  CTGCAG              
SmaI          103  CCCGGG               CCC^GGG
              873  CCCGGG              
XcmI          128  CCACGGCTTTTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1119  CCAGGACTTCAGTGG     
XmaCI         103  CCCGGG               C^CCGGG
              873  CCCGGG              
XmaI          103  CCCGGG               C^CCGGG
              873  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AspHI       
AvaII       BanII       BbvI        BcnI        BfaI        
BmyI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        MaeI        MaeIII      MboI        
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TfiI        TseI        TspRI       
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