################################## # NO CUT ENZYMES ( 204 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MslI MunI MvaI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AfaI 204 GTAC GT^AC AflIII 221 ACATGT A^C[GA][CT]GT BcnI 326 CCCGG CC^[GC]GG BsaJI 353 CCTTGG C^C[ACGT][ACGT]GG BsiYI 326 CCCGGAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 326 CCCGGAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 22 GAGAC GTCTC(1/5) BsmI 267 GAATGC GAATGC(1/-1) BspLU11I 221 ACATGT A^CATGT BsrDI 75 GCAATG GCAATG(2/0) BsrI 170 ACTGG ACTGG(1/-1) BssKI 326 CCCGG ^CC[ACGT]GG BstF5I 335 GGATG GGATG(2/0) Cac8I 348 GCAAGC GC[ACGT]^[ACGT]GC CfoI 339 GCGC GCG^C EaeI 2 TGGCCG [CT]^GGCC[GA] EcoT14I 353 CCTTGG C^C[AT][AT]GG FokI 335 GGATG GGATG(9/13) HaeIII 3 GGCC GG^CC HapII 327 CCGG C^CGG HhaI 339 GCGC GCG^C HinP1I 339 GCGC G^CGC HindIII 137 AAGCTT A^AGCTT HinfI 303 GATTC G^A[ACGT]TC HpaII 327 CCGG C^CGG HphI 264 GGTGA GGTGA(8/7) MaeIII 122 GTTAC ^GT[ACGT]AC MspA1I 5 CCGCTG C[AC]G^C[GT]G MspI 327 CCGG C^CGG NciI 326 CCCGG CC^[GC]GG NlaIII 222 CATG CATG^ NspI 221 ACATGT [GA]CATG^[CT] RsaI 204 GTAC GT^AC ScrFI 326 CCCGG CC^[ACGT]GG StyI 353 CCTTGG C^C[AT][AT]GG TaqI 42 TCGA T^CGA TfiI 303 GATTC G^A[AT]TC TthHB8I 42 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### AciI 5 CCGC CCGC(-3/-1) 333 GCGG AcsI 67 GAATTT [GA]^AATT[CT] 283 AAATTT ApoI 67 GAATTT [GA]^AATT[CT] 283 AAATTT DdeI 209 CTCAG C^T[ACGT]AG 272 CTCAG MnlI 208 CCTC CCTC(7/6) 119 GAGG SfaNI 31 GATGC GCATC(5/9) 336 GATGC TspRI 211 CAGTG CA[GC]TG[ACGT][ACGT]^ 274 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AluI BbvI Fnu4HI ItaI MseI MwoI Tru9I TseI Tsp509I