Restriction map for pF1KB7641
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AgeI        AhdI        
AlwI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaII       AviII       AvrII       BalI        BamHI       
BbsI        BciVI       BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsaI        BsaWI       BseAI       BsiWI       BsmAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     CelII       ClaI        CpoI        
DpnII       DraI        DrdI        Eam1105I    EarI        
Eco47III    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        Ksp632I     MamI        
MboI        MboII       MfeI        MflI        MluNI       
MroI        MscI        MseI        MslI        MunI        
NcoI        NdeI        NdeII       NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SalI        SapI        Sau3AI      SbfI        
ScaI        SfcI        SfiI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TaqI        Tru9I       
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AflIII        746  ACGCGT               A^C[GA][CT]GT
Alw44I        157  GTGCAC               G^TGCAC
AlwNI         199  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         157  GTGCAC               G^TGCAC
BbrPI         871  CACGTG               CAC^GTG
BbvCI         421  GCTGAGG              CCTCAGC(-5/-2)
BcgI          848  GCAAGGGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          514  CTAG                 C^TAG
BpmI          283  CTGGAG               CTGGAG(16/14)
BsaAI         871  CACGTG               [CT]AC^GT[GA]
BsgI          240  GTGCAG               GTGCAG(16/14)
BsiEI         897  CGGCCG               CG[GA][CT]^CG
BsrBI         258  GAGCGG               CCGCTC(-3/-3)
DraIII        787  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          897  CGGCCG               C^GGCCG
EclXI         897  CGGCCG               C^GGCCG
Eco52I        897  CGGCCG               C^GGCCG
EcoNI         279  CCTGCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HphI          573  TCACC                GGTGA(8/7)
KspI          100  CCGCGG               CCGC^GG
MaeI          514  CTAG                 C^TAG
MaeIII        349  GTGAC                ^GT[ACGT]AC
MluI          746  ACGCGT               A^CGCGT
PmaCI         871  CACGTG               CAC^GTG
PmlI          871  CACGTG               CAC^GTG
SacI          289  GAGCTC               GAGCT^C
SacII         100  CCGCGG               CCGC^GG
SexAI         788  ACCTGGT              A^CC[AT]GGT
SfaNI         890  GCATC                GCATC(5/9)
TfiI          396  GATTC                G^A[AT]TC
Tsp45I        349  GTGAC                ^GT[GC]AC
TspRI         387  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AocI          340  CCTCAGG              CC^T[ACGT]AGG
              401  CCTTAGG             
BglI          412  GCCCCTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              596  GCCACTCGGGC         
BseRI         265  GAGGAG               GAGGAG(10/8)
              286  GAGGAG              
BspMI         575  ACCTGC               ACCTGC(4/8)
              659  ACCTGC              
BsrI          140  ACTGG                ACTGG(1/-1)
              776  ACTGG               
BssHII        209  GCGCGC               G^CGCGC
              914  GCGCGC              
BstAPI        786  GCACCTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              870  GCACGTGCTGC         
Bsu36I        340  CCTCAGG              CC^T[ACGT]AGG
              401  CCTTAGG             
EaeI          897  CGGCCG               [CT]^GGCC[GA]
              908  CGGCCA              
Eco81I        340  CCTCAGG              CC^T[ACGT]AGG
              401  CCTTAGG             
EcoT14I       164  CCAAGG               C^C[AT][AT]GG
              971  CCTTGG              
NaeI          245  GCCGGC               GCC^GGC
              429  GCCGGC              
NgoMIV        245  GCCGGC               G^CCGGC
              429  GCCGGC              
PleI           49  GACTC                GAGTC(4/5)
              442  GACTC               
SmaI          522  CCCGGG               CCC^GGG
              703  CCCGGG              
StuI          230  AGGCCT               AGG^CCT
              638  AGGCCT              
StyI          164  CCAAGG               C^C[AT][AT]GG
              971  CCTTGG              
XmaCI         522  CCCGGG               C^CCGGG
              703  CCCGGG              
XmaI          522  CCCGGG               C^CCGGG
              703  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        ApaI        
AspHI       AvaI        BanI        BanII       BbeI        
BbvI        BcnI        BmyI        BsaHI       BsaJI       
BsiHKAI     BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BsrFI       BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DraII       DsaI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       ItaI        KasI        MaeII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NlaIV       Sau96I      
ScrFI       SfoI        TseI        
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