Restriction map for pF1KB7641
 (Linear) (Six-base) MAPSORT of: KIBB7641.seq  Check: 8788  from: 1  to: 978



KIBB7641.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     584     668     978 
   Size:        584      84     310

  Fragments arranged by size: 

                 584     310      84
 
AflIII A'CryG_T

Cuts at:      0     746     978 
   Size:        746     232
 
AlwNI CAG_nnn'CTG

Cuts at:      0     204     978 
   Size:        204     774
 
ApaI G_GGCC'C

Cuts at:      0     100     361     710     940     978 
   Size:        100     261     349     230      38

  Fragments arranged by size: 

                 349     261     230     100      38
 
ApaLI G'TGCA_C

Cuts at:      0     157     978 
   Size:        157     821
 
AvaI C'yCGr_G

Cuts at:      0     151     522     541     600     703     813     978 
   Size:        151     371      19      59     103     110     165

  Fragments arranged by size: 

                 371     165     151     110     103      59      19
 
BanI G'GyrC_C

Cuts at:      0      12      59     336     406     432     492     526     665 
   Size:         12      47     277      70      26      60      34     139

Cuts at:    665     860     978 
   Size:        195     118

  Fragments arranged by size: 

                 277     195     139     118      70      60      47      34
                  26      12
 
BanII G_rGCy'C

Cuts at:      0     100     293     361     476     710     940     978 
   Size:        100     193      68     115     234     230      38

  Fragments arranged by size: 

                 234     230     193     115     100      68      38
 
BbeI G_GCGC'C

Cuts at:      0     436     496     669     864     978 
   Size:        436      60     173     195     114

  Fragments arranged by size: 

                 436     195     173     114      60
 
BbvCI CC'TCA_GC

Cuts at:      0     422     978 
   Size:        422     556
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     837     871     978 
   Size:        837      34     107

  Fragments arranged by size: 

                 837     107      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     418     602     978 
   Size:        418     184     376

  Fragments arranged by size: 

                 418     376     184
 
Bme1580I G_kGCm'C

Cuts at:      0      17     100     161     361     529     710     940     978 
   Size:         17      83      61     200     168     181     230      38

  Fragments arranged by size: 

                 230     200     181     168      83      61      38      17
            
 
BmgBI CAC'GTC

Cuts at:      0     533     978 
   Size:        533     445
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     356     978 
   Size:        356     622
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     978 
   Size:        304     674
 
Bpu10I CC'TnA_GC

Cuts at:      0     205     422     978 
   Size:        205     217     556

  Fragments arranged by size: 

                 556     217     205
 
BsaAI yAC'GTr

Cuts at:      0     873     978 
   Size:        873     105
 
BsaHI Gr'CG_yC

Cuts at:      0     433     493     666     861     978 
   Size:        433      60     173     195     117

  Fragments arranged by size: 

                 433     195     173     117      60
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     160     190     978 
   Size:        160      30     788

  Fragments arranged by size: 

                 788     160      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     280     301     978 
   Size:        280      21     677

  Fragments arranged by size: 

                 677     280      21
 
BseYI C'CCAG_C

Cuts at:      0     111     475     836     978 
   Size:        111     364     361     142

  Fragments arranged by size: 

                 364     361     142     111
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     261     978 
   Size:        261     717
 
BsiEI CG_ry'CG

Cuts at:      0     900     978 
   Size:        900      78
 
BsiHKAI G_wGCw'C

Cuts at:      0     161     293     659     788     872     978 
   Size:        161     132     366     129      84     106

  Fragments arranged by size: 

                 366     161     132     129     106      84
 
Bsp1286I G_dGCh'C

Cuts at:      0      17     100     161     293     361     476     529     659 
   Size:         17      83      61     132      68     115      53     130

Cuts at:    659     710     788     872     940     978 
   Size:         51      78      84      68      38

  Fragments arranged by size: 

                 132     130     115      84      83      78      68      68
                  61      53      51      38      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     584     668     978 
   Size:        584      84     310

  Fragments arranged by size: 

                 584     310      84
 
BsrBI CCG'CTC

Cuts at:      0     260     978 
   Size:        260     718
 
BsrFI r'CCGG_y

Cuts at:      0     245     429     587     978 
   Size:        245     184     158     391

  Fragments arranged by size: 

                 391     245     184     158
 
BssHII G'CGCG_C

Cuts at:      0     209     914     978 
   Size:        209     705      64

  Fragments arranged by size: 

                 705     209      64
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     792     876     978 
   Size:        792      84     102

  Fragments arranged by size: 

                 792     102      84
 
Bsu36I CC'TnA_GG

Cuts at:      0     341     402     978 
   Size:        341      61     576

  Fragments arranged by size: 

                 576     341      61
 
BtgI C'CryG_G

Cuts at:      0      55     100     894     978 
   Size:         55      45     794      84

  Fragments arranged by size: 

                 794      84      55      45
 
DraIII CAC_nnn'GTG

Cuts at:      0     792     978 
   Size:        792     186
 
EaeI y'GGCC_r

Cuts at:      0     897     908     978 
   Size:        897      11      70

  Fragments arranged by size: 

                 897      70      11
 
EagI C'GGCC_G

Cuts at:      0     897     978 
   Size:        897      81
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      58     978 
   Size:         58     920
 
EcoICRI GAG'CTC

Cuts at:      0     291     978 
   Size:        291     687
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     978 
   Size:        304     674
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     283     978 
   Size:        283     695
 
EcoO109I rG'GnC_Cy

Cuts at:      0      36     357     358     952     978 
   Size:         36     321       1     594      26

  Fragments arranged by size: 

                 594     321      36      26       1
 
HaeII r_GCGC'y

Cuts at:      0      46     112     436     496     669     864     978 
   Size:         46      66     324      60     173     195     114

  Fragments arranged by size: 

                 324     195     173     114      66      60      46
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     307     339     978 
   Size:        307      32     639

  Fragments arranged by size: 

                 639     307      32
 
KasI G'GCGC_C

Cuts at:      0     432     492     665     860     978 
   Size:        432      60     173     195     118

  Fragments arranged by size: 

                 432     195     173     118      60
 
MluI A'CGCG_T

Cuts at:      0     746     978 
   Size:        746     232
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     978 
   Size:        158     820
 
MspA1I CmG'CkG

Cuts at:      0     102     489     907     978 
   Size:        102     387     418      71

  Fragments arranged by size: 

                 418     387     102      71
 
NaeI GCC'GGC

Cuts at:      0     247     431     978 
   Size:        247     184     547

  Fragments arranged by size: 

                 547     247     184
 
NarI GG'CG_CC

Cuts at:      0     433     493     666     861     978 
   Size:        433      60     173     195     117

  Fragments arranged by size: 

                 433     195     173     117      60
 
NgoMIV G'CCGG_C

Cuts at:      0     245     429     978 
   Size:        245     184     549

  Fragments arranged by size: 

                 549     245     184
 
PasI CC'CwG_GG

Cuts at:      0     361     415     978 
   Size:        361      54     563

  Fragments arranged by size: 

                 563     361      54
 
PmlI CAC'GTG

Cuts at:      0     873     978 
   Size:        873     105
 
PspOMI G'GGCC_C

Cuts at:      0      96     357     706     936     978 
   Size:         96     261     349     230      42

  Fragments arranged by size: 

                 349     261     230      96      42
 
SacI G_AGCT'C

Cuts at:      0     293     978 
   Size:        293     685
 
SacII CC_GC'GG

Cuts at:      0     103     978 
   Size:        103     875
 
SexAI A'CCwGG_T

Cuts at:      0     788     978 
   Size:        788     190
 
SfoI GGC'GCC

Cuts at:      0     434     494     667     862     978 
   Size:        434      60     173     195     116

  Fragments arranged by size: 

                 434     195     173     116      60
 
SmaI CCC'GGG

Cuts at:      0     524     705     978 
   Size:        524     181     273

  Fragments arranged by size: 

                 524     273     181
 
StuI AGG'CCT

Cuts at:      0     232     640     978 
   Size:        232     408     338

  Fragments arranged by size: 

                 408     338     232
 
StyI C'CwwG_G

Cuts at:      0     164     971     978 
   Size:        164     807       7

  Fragments arranged by size: 

                 807     164       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     225     978 
   Size:        225     753
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      40      72     978 
   Size:         40      32     906

  Fragments arranged by size: 

                 906      40      32
 
XmaI C'CCGG_G

Cuts at:      0     522     703     978 
   Size:        522     181     275

  Fragments arranged by size: 

                 522     275     181
 

 Enzymes that do cut:

     AarI   AflIII    AlwNI     ApaI    ApaLI     AvaI     BanI    BanII
     BbeI    BbvCI     BcgI     BglI Bme1580I    BmgBI     BplI     BpmI
   Bpu10I    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI   BssHII   BstAPI   Bsu36I
     BtgI   DraIII     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I     KasI     MluI     MmeI   MspA1I     NaeI
     NarI   NgoMIV     PasI     PmlI   PspOMI     SacI    SacII    SexAI
     SfoI     SmaI     StuI     StyI    TaqII     TstI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI    BciVI     BclI    BfrBI    BglII
     BlpI     BmrI     BmtI    BpuEI     BsaI    BsaBI    BsaWI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EarI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MscI     MslI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfiI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI
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