Restriction map for pF1KF0386
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        AlwI        AocI        
Aor51HI     AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BbrPI       BciVI       BfaI        
BfrI        BglII       BlnI        BsaAI       BsaBI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BsrDI       BsrGI       BssHII      BstBI       BstYI       
Bsu36I      ClaI        CpoI        DraIII      DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoRI       EcoRV       EcoT14I     
FseI        FspI        HincII      HindII      HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MflI        MluI        MroI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StyI        SwaI        TfiI        
Tth111I     XbaI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI          795  GTATAC               GT^[AC][GT]AC
AccII        1142  CGCG                 CG^CG
AcsI          814  AAATTT               [GA]^AATT[CT]
AcyI         1888  GACGCC               G[GA]^CG[CT]C
ApaI         1985  GGGCCC               GGGCC^C
ApoI          814  AAATTT               [GA]^AATT[CT]
AvaI          152  CCCGGG               C^[CT]CG[GA]G
BbvCI        1237  GCTGAGG              CCTCAGC(-5/-2)
BcgI          830  CGATGGTGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1304  TGATCA               T^GATCA
BlpI          352  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      352  GCTCAGC              GC^T[ACGT]AGC
BsaHI        1888  GACGCC               G[GA]^CG[CT]C
BsaI           19  GAGACC               GGTCTC(1/5)
BseRI        1240  GAGGAG               GAGGAG(10/8)
BsoBI         152  CCCGGG               C^[CT]CG[GA]G
BspHI        1211  TCATGA               T^CATGA
BsrFI        1871  ACCGGC               [GA]^CCGG[CT]
BssSI         172  CTCGTG               CACGAG(-5/-1)
Bst1107I      795  GTATAC               GTA^TAC
BstEII       1962  GGTCACC              G^GT[ACGT]ACC
BstPI        1962  GGTCACC              G^GT[ACGT]ACC
BstUI        1142  CGCG                 CG^CG
BstXI        1718  CCAAGTTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       795  GTATAC               GTA^TAC
CelII         352  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1871  ACCGGC               [GA]^CCGG[CT]
DraI         1182  TTTAAA               TTT^AAA
DrdI          987  GACCCCGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1356  GAAGAG               CTCTTC(1/4)
EcoO65I      1962  GGTCACC              G^GT[ACGT]ACC
EcoT22I       655  ATGCAT               ATGCA^T
FbaI         1304  TGATCA               T^GATCA
Hin1I        1888  GACGCC               G[GA]^CG[CT]C
HindIII       224  AAGCTT               A^AGCTT
HinfI         923  GAGTC                G^A[ACGT]TC
Ksp632I      1356  GAAGAG               CTCTTC(1/4)
MvnI         1142  CGCG                 CG^CG
NsiI          655  ATGCAT               ATGCA^T
PleI          923  GAGTC                GAGTC(4/5)
RcaI         1211  TCATGA               T^CATGA
SapI         1356  GAAGAGC              GCTCTTC(1/4)
SmaI          152  CCCGGG               CCC^GGG
StuI         2003  AGGCCT               AGG^CCT
XcmI          110  CCAGCAAGGATATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         152  CCCGGG               C^CCGGG
XmaI          152  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

Alw44I       1635  GTGCAC               G^TGCAC
             1912  GTGCAC              
ApaLI        1635  GTGCAC               G^TGCAC
             1912  GTGCAC              
BanI         1458  GGTGCC               G^G[CT][GA]CC
             1482  GGTGCC              
BglI         1217  GCCCAGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1528  GCCGTCCTGGC         
BsmAI          19  GAGAC                GTCTC(1/5)
              200  GAGAC               
BspMI         255  GCAGGT               ACCTGC(4/8)
              978  GCAGGT              
DraII        1340  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1796  AGGACCT             
EcoO109I     1340  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1796  AGGACCT             
HaeII         261  GGCGCT               [GA]GCGC^[CT]
              312  GGCGCT              
HgaI         1888  GACGC                GACGC(5/10)
             1994  GCGTC               
MaeII           5  ACGT                 A^CGT
             1061  ACGT                
MseI          440  TTAA                 T^TAA
             1183  TTAA                
MslI         1188  CACACCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1452  CATGGCGGTG          
PpuMI        1340  AGGACCT              [GA]G^G[AT]CC[CT]
             1796  AGGACCT             
SacI          824  GAGCTC               GAGCT^C
             1822  GAGCTC              
Tru9I         440  TTAA                 T^TAA
             1183  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AspHI       
AvaII       BalI        BanII       BbsI        BbvI        
BcnI        BmyI        BpmI        BpuAI       BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BsrBI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EaeI        EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        TaqI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
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