Restriction map for pF1KF0386
 (Linear) (Six-base) MAPSORT of: FLJ00386.seq  Check: 4997  from: 1  to: 2022



FLJ00386.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     246     969    2022 
   Size:        246     723    1053

  Fragments arranged by size: 

                1053     723     246
 
AccI GT'mk_AC

Cuts at:      0     796    2022 
   Size:        796    1226
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160    1143    1285    1423    2022 
   Size:        160     983     142     138     599

  Fragments arranged by size: 

                 983     599     160     142     138
 
AleI CACnn'nnGTG

Cuts at:      0    1192    2022 
   Size:       1192     830
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1705    1737    2022 
   Size:       1705      32     285

  Fragments arranged by size: 

                1705     285      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      54     489     633     645    1254    2022 
   Size:         54     435     144      12     609     768

  Fragments arranged by size: 

                 768     609     435     144      54      12
 
ApaI G_GGCC'C

Cuts at:      0    1989    2022 
   Size:       1989      33
 
ApaLI G'TGCA_C

Cuts at:      0    1635    1912    2022 
   Size:       1635     277     110

  Fragments arranged by size: 

                1635     277     110
 
ApoI r'AATT_y

Cuts at:      0     814    2022 
   Size:        814    1208
 
AvaI C'yCGr_G

Cuts at:      0     152    2022 
   Size:        152    1870
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1486    1519    2022 
   Size:       1486      33     503

  Fragments arranged by size: 

                1486     503      33
 
BanI G'GyrC_C

Cuts at:      0    1458    1482    2022 
   Size:       1458      24     540

  Fragments arranged by size: 

                1458     540      24
 
BanII G_rGCy'C

Cuts at:      0     828    1132    1219    1292    1826    1989    2022 
   Size:        828     304      87      73     534     163      33

  Fragments arranged by size: 

                 828     534     304     163      87      73      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1334    1411    1548    2022 
   Size:       1334      77     137     474

  Fragments arranged by size: 

                1334     474     137      77
 
BbvCI CC'TCA_GC

Cuts at:      0    1238    2022 
   Size:       1238     784
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     819     853    2022 
   Size:        819      34    1169

  Fragments arranged by size: 

                1169     819      34
 
BclI T'GATC_A

Cuts at:      0    1304    2022 
   Size:       1304     718
 
BfrBI ATG'CAT

Cuts at:      0     657    2022 
   Size:        657    1365
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1223    1534    2022 
   Size:       1223     311     488

  Fragments arranged by size: 

                1223     488     311
 
BlpI GC'TnA_GC

Cuts at:      0     353    2022 
   Size:        353    1669
 
Bme1580I G_kGCm'C

Cuts at:      0     240    1463    1639    1835    1916    1989    2022 
   Size:        240    1223     176     196      81      73      33

  Fragments arranged by size: 

                1223     240     196     176      81      73      33
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     844    1082    1126    1621    1676    2022 
   Size:        844     238      44     495      55     346

  Fragments arranged by size: 

                 844     495     346     238      55      44
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     394     686    1212    1453    1675    1840    1888 
   Size:        235     159     292     526     241     222     165      48

Cuts at:   1888    1914    2022 
   Size:         26     108

  Fragments arranged by size: 

                 526     292     241     235     222     165     159     108
                  48      26
 
Bpu10I CC'TnA_GC

Cuts at:      0     896    1238    2022 
   Size:        896     342     784

  Fragments arranged by size: 

                 896     784     342
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206    1426    1675    2022 
   Size:        206    1220     249     347

  Fragments arranged by size: 

                1220     347     249     206
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      13    2022 
   Size:         13    2009
 
BsaHI Gr'CG_yC

Cuts at:      0    1889    2022 
   Size:       1889     133
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     914     944    1234    1264    2022 
   Size:        914      30     290      30     758

  Fragments arranged by size: 

                 914     758     290      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1255    2022 
   Size:       1255     767
 
BseYI C'CCAG_C

Cuts at:      0     239     575     638     692     749    1013    1039    1102 
   Size:        239     336      63      54      57     264      26      63

Cuts at:   1102    1201    1218    2022 
   Size:         99      17     804

  Fragments arranged by size: 

                 804     336     264     239      99      63      63      57
                  54      26      17
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     859     874     886    1003    2022 
   Size:        412     447      15      12     117    1019

  Fragments arranged by size: 

                1019     447     412     117      15      12
 
BsiHKAI G_wGCw'C

Cuts at:      0      37     828    1639    1691    1826    1916    2022 
   Size:         37     791     811      52     135      90     106

  Fragments arranged by size: 

                 811     791     135     106      90      52      37
 
Bsp1286I G_dGCh'C

Cuts at:      0      37     240     828    1132    1219    1292    1463    1639 
   Size:         37     203     588     304      87      73     171     176

Cuts at:   1639    1691    1826    1835    1916    1989    2022 
   Size:         52     135       9      81      73      33

  Fragments arranged by size: 

                 588     304     203     176     171     135      87      81
                  73      73      52      37      33       9
 
BspHI T'CATG_A

Cuts at:      0    1211    2022 
   Size:       1211     811
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     246     969    2022 
   Size:        246     723    1053

  Fragments arranged by size: 

                1053     723     246
 
BsrBI CCG'CTC

Cuts at:      0    1518    1547    1668    2022 
   Size:       1518      29     121     354

  Fragments arranged by size: 

                1518     354     121      29
 
BsrFI r'CCGG_y

Cuts at:      0    1871    2022 
   Size:       1871     151
 
BssSI C'ACGA_G

Cuts at:      0     172    2022 
   Size:        172    1850
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     567     654     999    2022 
   Size:        567      87     345    1023

  Fragments arranged by size: 

                1023     567     345      87
 
BstEII G'GTnAC_C

Cuts at:      0    1962    2022 
   Size:       1962      60
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1725    2022 
   Size:       1725     297
 
BstZ17I GTA'TAC

Cuts at:      0     797    2022 
   Size:        797    1225
 
BtsI GCAGTG_nn'

Cuts at:      0    1746    1882    2022 
   Size:       1746     136     140

  Fragments arranged by size: 

                1746     140     136
 
DraI TTT'AAA

Cuts at:      0    1184    2022 
   Size:       1184     838
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     993    2022 
   Size:        993    1029
 
EaeI y'GGCC_r

Cuts at:      0     176    1032    1535    1703    2022 
   Size:        176     856     503     168     319

  Fragments arranged by size: 

                 856     503     319     176     168
 
EarI CTCTTCn'nnn_

Cuts at:      0    1351    2022 
   Size:       1351     671
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      45     608    2022 
   Size:         45     563    1414

  Fragments arranged by size: 

                1414     563      45
 
EcoICRI GAG'CTC

Cuts at:      0     826    1824    2022 
   Size:        826     998     198

  Fragments arranged by size: 

                 998     826     198
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     235     394     686    1143    1212    1285    1423 
   Size:        160      75     159     292     457      69      73     138

Cuts at:   1423    1453    1675    1840    1888    1914    2022 
   Size:         30     222     165      48      26     108

  Fragments arranged by size: 

                 457     292     222     165     160     159     138     108
                  75      73      69      48      30      26
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1341    1797    2022 
   Size:       1341     456     225

  Fragments arranged by size: 

                1341     456     225
 
HaeII r_GCGC'y

Cuts at:      0     265     316    2022 
   Size:        265      51    1706

  Fragments arranged by size: 

                1706     265      51
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     900     914     932     946     990    1022    1942    1974 
   Size:        900      14      18      14      44      32     920      32

Cuts at:   1974    2022 
   Size:         48

  Fragments arranged by size: 

                 920     900      48      44      32      32      18      14
                  14
 
HindIII A'AGCT_T

Cuts at:      0     224    2022 
   Size:        224    1798
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     540    1082    2022 
   Size:        540     542     940

  Fragments arranged by size: 

                 940     542     540
 
MscI TGG'CCA

Cuts at:      0     178    1537    1705    2022 
   Size:        178    1359     168     317

  Fragments arranged by size: 

                1359     317     178     168
 
MslI CAynn'nnrTG

Cuts at:      0    1192    1456    2022 
   Size:       1192     264     566

  Fragments arranged by size: 

                1192     566     264
 
MspA1I CmG'CkG

Cuts at:      0     243     489     507     645    1222    1272    1837    1860 
   Size:        243     246      18     138     577      50     565      23

Cuts at:   1860    1919    2022 
   Size:         59     103

  Fragments arranged by size: 

                 577     565     246     243     138     103      59      50
                  23      18
 
NsiI A_TGCA'T

Cuts at:      0     659    2022 
   Size:        659    1363
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     258     756     912    2022 
   Size:        258     498     156    1110

  Fragments arranged by size: 

                1110     498     258     156
 
PfoI T'CCnGG_A

Cuts at:      0     918    1520    1946    2022 
   Size:        918     602     426      76

  Fragments arranged by size: 

                 918     602     426      76
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1189    1221    2022 
   Size:       1189      32     801

  Fragments arranged by size: 

                1189     801      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1341    1797    2022 
   Size:       1341     456     225

  Fragments arranged by size: 

                1341     456     225
 
PspOMI G'GGCC_C

Cuts at:      0    1985    2022 
   Size:       1985      37
 
PstI C_TGCA'G

Cuts at:      0     248     269     494     512     650    1753    1943    2022 
   Size:        248      21     225      18     138    1103     190      79

  Fragments arranged by size: 

                1103     248     225     190     138      79      21      18
            
 
PvuII CAG'CTG

Cuts at:      0     243     489     507     645    1222    1272    2022 
   Size:        243     246      18     138     577      50     750

  Fragments arranged by size: 

                 750     577     246     243     138      50      18
 
SacI G_AGCT'C

Cuts at:      0     828    1826    2022 
   Size:        828     998     196

  Fragments arranged by size: 

                 998     828     196
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1351    2022 
   Size:       1351     671
 
SfcI C'TryA_G

Cuts at:      0     244     265     316     490     508     646    1749    1939 
   Size:        244      21      51     174      18     138    1103     190

Cuts at:   1939    2022 
   Size:         83

  Fragments arranged by size: 

                1103     244     190     174     138      83      51      21
                  18
 
SmaI CCC'GGG

Cuts at:      0     154    2022 
   Size:        154    1868
 
SmlI C'TyrA_G

Cuts at:      0     221    1441    1654    2022 
   Size:        221    1220     213     368

  Fragments arranged by size: 

                1220     368     221     213
 
StuI AGG'CCT

Cuts at:      0    2005    2022 
   Size:       2005      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      37    1629    1950    2022 
   Size:         37    1592     321      72

  Fragments arranged by size: 

                1592     321      72      37
 
TatI w'GTAC_w

Cuts at:      0     885    1280    2022 
   Size:        885     395     742

  Fragments arranged by size: 

                 885     742     395
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      75      91     107     123    1569    1601    2022 
   Size:         75      16      16      16    1446      32     421

  Fragments arranged by size: 

                1446     421      75      32      16      16      16
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     117    2022 
   Size:        117    1905
 
XmaI C'CCGG_G

Cuts at:      0     152    2022 
   Size:        152    1870
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BaeI     BanI    BanII     BbsI    BbvCI     BcgI
     BclI    BfrBI     BglI     BlpI Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BssSI   BstAPI   BstEII
    BstXI  BstZ17I     BtsI     DraI     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I  HindIII     MmeI     MscI
     MslI   MspA1I     NsiI    PflMI     PfoI     PpiI    PpuMI   PspOMI
     PstI    PvuII     SacI     SapI     SfcI     SmaI     SmlI     StuI
    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AscI     AseI    AsiSI    AvrII    BamHI     BbeI    BciVI
    BglII    BmgBI     BmtI     BplI    BsaAI    BsaBI    BsaWI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BsrDI    BsrGI   BssHII    BstBI
    BstYI   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     EagI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI  Tth111I     XbaI     XhoI     XmnI     ZraI
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