Restriction map for pF1KF0343
##################################
# NO CUT ENZYMES (   54 enzymes) #
##################################

AccIII      AflII       AgeI        AhdI        Aor51HI     
AscI        AseI        AsnI        AspEI       AviII       
BciVI       BfrI        BseAI       BsiWI       BspEI       
BssHII      CpoI        DraI        Eam1105I    Eco47III    
EcoRV       EcoT22I     FseI        FspI        HindIII     
HpaI        KspI        MfeI        MluI        MroI        
MseI        MunI        NdeI        NheI        NotI        
NruI        NsiI        PacI        PinAI       PmeI        
PshBI       RsrII       SacI        SacII       SbfI        
SgrAI       SpeI        SphI        Sse8387I    SspI        
SwaI        TfiI        Tru9I       XbaI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

Acc65I       4587  GGTACC               G^GTACC
AclI         6439  AACGTT               AA^CGTT
AcsI         6748  GAATTC               [GA]^AATT[CT]
ApoI         6748  GAATTC               [GA]^AATT[CT]
Asp718I      4587  GGTACC               G^GTACC
AspI         5350  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        6672  CCTAGG               C^CTAGG
BlnI         6672  CCTAGG               C^CTAGG
BsaBI        5499  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI         5774  GCATTC               GAATGC(1/-1)
BspLU11I     3361  ACATGT               A^CATGT
BstBI        1696  TTCGAA               TT^CGAA
EcoRI        6748  GAATTC               G^AATTC
KpnI         4587  GGTACC               GGTAC^C
MamI         5499  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspV         1696  TTCGAA               TT^CGAA
PflFI        5350  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        4006  GACGGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     6439  AACGTT               AA^CGTT
PvuI         7064  CGATCG               CGAT^CG
SalI           61  GTCGAC               G^TCGAC
SapI         7698  GAAGAGC              GCTCTTC(1/4)
ScaI         4566  AGTACT               AGT^ACT
SexAI        6032  ACCTGGT              A^CC[AT]GGT
SfiI         3882  GGCCCGTGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         1696  TTCGAA               TT^CGAA
SnaBI        7687  TACGTA               TAC^GTA
Tth111I      5350  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AflIII        317  ACGTGT               A^C[GA][CT]GT
             3361  ACATGT              
Asp700I       138  GAACACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             6916  GAACACATTC          
BbeI          595  GGCGCC               GGCGC^C
             3280  GGCGCC              
BsaWI        3766  TCCGGT               [AT]^CCGG[AT]
             3862  ACCGGA              
BspDI        4708  ATCGAT               AT^CGAT
             7066  ATCGAT              
BsrBI         579  GAGCGG               CCGCTC(-3/-3)
             3511  GAGCGG              
Bst1107I     2320  GTATAC               GTA^TAC
             4809  GTATAC              
BstAPI       5033  GCACCGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             6030  GCACCTGGTGC         
BstZ17I      2320  GTATAC               GTA^TAC
             4809  GTATAC              
ClaI         4708  ATCGAT               AT^CGAT
             7066  ATCGAT              
EcoNI         102  CCTGGCGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             7830  CCTGCTCAAGG         
KasI          595  GGCGCC               G^GCGCC
             3280  GGCGCC              
NarI          595  GGCGCC               GG^CGCC
             3280  GGCGCC              
PflMI        1658  CCATCACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             6057  CCAGCACGTGG         
SfoI          595  GGCGCC               GGC^GCC
             3280  GGCGCC              
Van91I       1658  CCATCACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             6057  CCAGCACGTGG         
XmnI          138  GAACACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             6916  GAACACATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  159 enzymes) #
#########################################

AatII       AccI        AccII       AciI        AcyI        
AfaI        AluI        Alw44I      AlwI        AlwNI       
AocI        ApaI        ApaLI       AspHI       AvaI        
AvaII       BalI        BamHI       BanI        BanII       
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BclI        BcnI        BfaI        BglI        BglII       
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
Bsp1407I    BspHI       BspMI       BsrDI       BsrFI       
BsrGI       BsrI        BssKI       BssSI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
EarI        EclXI       Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PaeR7I      
PleI        PmaCI       PmlI        PpuMI       PstI        
PvuII       RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
SspBI       StuI        StyI        TaqI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoI        XhoII       XmaCI       XmaI        
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