Restriction map for pF1KF0068
 (Linear) (Six-base) MAPSORT of: FLJ00068.seq  Check: 3473  from: 1  to: 3573



FLJ00068.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1137    1401    2007    3080    3573 
   Size:       1137     264     606    1073     493

  Fragments arranged by size: 

                1137    1073     606     493     264
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     640     886    1231    1668    1960    2847    3229    3573 
   Size:        640     246     345     437     292     887     382     344

  Fragments arranged by size: 

                 887     640     437     382     345     344     292     246
            
 
AfeI AGC'GCT

Cuts at:      0    2914    3573 
   Size:       2914     659
 
AgeI A'CCGG_T

Cuts at:      0    1950    3573 
   Size:       1950    1623
 
AleI CACnn'nnGTG

Cuts at:      0    3013    3573 
   Size:       3013     560
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     424    2655    3573 
   Size:        424    2231     918

  Fragments arranged by size: 

                2231     918     424
 
AlwNI CAG_nnn'CTG

Cuts at:      0     735     843     915    1491    1545    1653    1770    2119 
   Size:        735     108      72     576      54     108     117     349

Cuts at:   2119    2628    3521    3540    3573 
   Size:        509     893      19      33

  Fragments arranged by size: 

                 893     735     576     509     349     117     108     108
                  72      54      33      19
 
ApaI G_GGCC'C

Cuts at:      0    2650    2885    3573 
   Size:       2650     235     688

  Fragments arranged by size: 

                2650     688     235
 
ApaLI G'TGCA_C

Cuts at:      0    2692    3573 
   Size:       2692     881
 
ApoI r'AATT_y

Cuts at:      0     205     764    1618    3573 
   Size:        205     559     854    1955

  Fragments arranged by size: 

                1955     854     559     205
 
AvaI C'yCGr_G

Cuts at:      0     599    1370    1749    2278    3230    3573 
   Size:        599     771     379     529     952     343

  Fragments arranged by size: 

                 952     771     599     529     379     343
 
BamHI G'GATC_C

Cuts at:      0     508    3573 
   Size:        508    3065
 
BanI G'GyrC_C

Cuts at:      0    1643    1790    1892    2128    2405    2813    3573 
   Size:       1643     147     102     236     277     408     760

  Fragments arranged by size: 

                1643     760     408     277     236     147     102
 
BanII G_rGCy'C

Cuts at:      0     427     800    1243    1448    1608    1833    1852    2250 
   Size:        427     373     443     205     160     225      19     398

Cuts at:   2250    2650    2663    2885    3573 
   Size:        400      13     222     688

  Fragments arranged by size: 

                 688     443     427     400     398     373     225     222
                 205     160      19      13
 
BbeI G_GCGC'C

Cuts at:      0    2817    3573 
   Size:       2817     756
 
BbvCI CC'TCA_GC

Cuts at:      0     337    1706    3422    3458    3573 
   Size:        337    1369    1716      36     115

  Fragments arranged by size: 

                1716    1369     337     115      36
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     215     249    3573 
   Size:        215      34    3324

  Fragments arranged by size: 

                3324     215      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3398    3573 
   Size:       3398     175
 
BglI GCCn_nnn'nGGC

Cuts at:      0     826     882    1190    3573 
   Size:        826      56     308    2383

  Fragments arranged by size: 

                2383     826     308      56
 
BglII A'GATC_T

Cuts at:      0    2978    3470    3573 
   Size:       2978     492     103

  Fragments arranged by size: 

                2978     492     103
 
BlpI GC'TnA_GC

Cuts at:      0     799    3573 
   Size:        799    2774
 
Bme1580I G_kGCm'C

Cuts at:      0     256     639    1047    1085    1587    2133    2303    2328 
   Size:        256     383     408      38     502     546     170      25

Cuts at:   2328    2519    2650    2696    2885    3573 
   Size:        191     131      46     189     688

  Fragments arranged by size: 

                 688     546     502     408     383     256     191     189
                 170     131      46      38      25
 
BmgBI CAC'GTC

Cuts at:      0    1700    3573 
   Size:       1700    1873
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     497     745    2557    2819    3573 
   Size:        497     248    1812     262     754

  Fragments arranged by size: 

                1812     754     497     262     248
 
BmtI G_CTAG'C

Cuts at:      0    1970    3573 
   Size:       1970    1603
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34      81     320     511     913    1036    1384    1450 
   Size:         34      47     239     191     402     123     348      66

Cuts at:   1450    1756    2141    2365    2410    3012    3320    3436    3486 
   Size:        306     385     224      45     602     308     116      50

Cuts at:   3486    3573 
   Size:         87

  Fragments arranged by size: 

                 602     402     385     348     308     306     239     224
                 191     123     116      87      66      50      47      45
                  34
 
Bpu10I CC'TnA_GC

Cuts at:      0     337     521    1237    1706    3422    3458    3573 
   Size:        337     184     716     469    1716      36     115

  Fragments arranged by size: 

                1716     716     469     337     184     115      36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     441    1948    2746    3573 
   Size:        441    1507     798     827

  Fragments arranged by size: 

                1507     827     798     441
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     455    2944    3296    3347    3376    3573 
   Size:        455    2489     352      51      29     197

  Fragments arranged by size: 

                2489     455     352     197      51      29
 
BsaBI GATnn'nnATC

Cuts at:      0    2039    3573 
   Size:       2039    1534
 
BsaHI Gr'CG_yC

Cuts at:      0    2735    2814    3235    3573 
   Size:       2735      79     421     338

  Fragments arranged by size: 

                2735     421     338      79
 
BsaWI w'CCGG_w

Cuts at:      0    1950    3573 
   Size:       1950    1623
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2142    2172    2221    2251    2554    2584    2899    2929 
   Size:       2142      30      49      30     303      30     315      30

Cuts at:   2929    3573 
   Size:        644

  Fragments arranged by size: 

                2142     644     315     303      49      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     115     190     258     974    1447    1723    2138    2863 
   Size:        115      75      68     716     473     276     415     725

Cuts at:   2863    3573 
   Size:        710

  Fragments arranged by size: 

                 725     716     710     473     415     276     115      75
                  68
 
BseYI C'CCAG_C

Cuts at:      0     219     645    1085    1438    1553    1607    1637    1845 
   Size:        219     426     440     353     115      54      30     208

Cuts at:   1845    1877    3166    3357    3391    3432    3573 
   Size:         32    1289     191      34      41     141

  Fragments arranged by size: 

                1289     440     426     353     219     208     191     141
                 115      54      41      34      32      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      96     687    1288    1369    1762    1979    2467    3325 
   Size:         96     591     601      81     393     217     488     858

Cuts at:   3325    3573 
   Size:        248

  Fragments arranged by size: 

                 858     601     591     488     393     248     217      96
                  81
 
BsiEI CG_ry'CG

Cuts at:      0    2037    3189    3573 
   Size:       2037    1152     384

  Fragments arranged by size: 

                2037    1152     384
 
BsiHKAI G_wGCw'C

Cuts at:      0     774    2663    2696    3573 
   Size:        774    1889      33     877

  Fragments arranged by size: 

                1889     877     774      33
 
Bsp1286I G_dGCh'C

Cuts at:      0     256     427     639     774     800    1047    1085    1243 
   Size:        256     171     212     135      26     247      38     158

Cuts at:   1243    1448    1587    1608    1833    1852    2133    2250    2303 
   Size:        205     139      21     225      19     281     117      53

Cuts at:   2303    2328    2519    2650    2663    2696    2885    3573 
   Size:         25     191     131      13      33     189     688

  Fragments arranged by size: 

                 688     281     256     247     225     212     205     191
                 189     171     158     139     135     131     117      53
                  38      33      26      25      21      19      13
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     117     710    1137    1401    1503    2007    2073    2585 
   Size:        117     593     427     264     102     504      66     512

Cuts at:   2585    2729    2975    3080    3347    3573 
   Size:        144     246     105     267     226

  Fragments arranged by size: 

                 593     512     504     427     267     264     246     226
                 144     117     105     102      66
 
BsrBI CCG'CTC

Cuts at:      0    2232    3573 
   Size:       2232    1341
 
BsrDI GCAATG_nn'

Cuts at:      0    1265    3158    3239    3573 
   Size:       1265    1893      81     334

  Fragments arranged by size: 

                1893    1265     334      81
 
BsrFI r'CCGG_y

Cuts at:      0    1950    3573 
   Size:       1950    1623
 
BsrGI T'GTAC_A

Cuts at:      0    3347    3573 
   Size:       3347     226
 
BssSI C'ACGA_G

Cuts at:      0    2414    3573 
   Size:       2414    1159
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1042    1149    1770    2781    3573 
   Size:       1042     107     621    1011     792

  Fragments arranged by size: 

                1042    1011     792     621     107
 
BstEII G'GTnAC_C

Cuts at:      0    1230    3573 
   Size:       1230    2343
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     579    1845    2335    3135    3573 
   Size:        579    1266     490     800     438

  Fragments arranged by size: 

                1266     800     579     490     438
 
BstYI r'GATC_y

Cuts at:      0     508    2978    3470    3573 
   Size:        508    2470     492     103

  Fragments arranged by size: 

                2470     508     492     103
 
Bsu36I CC'TnA_GG

Cuts at:      0    2014    3573 
   Size:       2014    1559
 
BtgI C'CryG_G

Cuts at:      0    1091    1162    1838    2225    2313    2729    2877    3128 
   Size:       1091      71     676     387      88     416     148     251

Cuts at:   3128    3573 
   Size:        445

  Fragments arranged by size: 

                1091     676     445     416     387     251     148      88
                  71
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     421    3573 
   Size:        421    3152
 
BtsI GCAGTG_nn'

Cuts at:      0     626    2073    2364    2611    3573 
   Size:        626    1447     291     247     962

  Fragments arranged by size: 

                1447     962     626     291     247
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2874    2909    3058    3093    3573 
   Size:       2874      35     149      35     480

  Fragments arranged by size: 

                2874     480     149      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     352    2948    3573 
   Size:        352    2596     625

  Fragments arranged by size: 

                2596     625     352
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3193    3573 
   Size:       3193     380
 
EaeI y'GGCC_r

Cuts at:      0     569     581    1420    1835    2087    2222    2726    3438 
   Size:        569      12     839     415     252     135     504     712

Cuts at:   3438    3573 
   Size:        135

  Fragments arranged by size: 

                 839     712     569     504     415     252     135     135
                  12
 
EarI CTCTTCn'nnn_

Cuts at:      0      92     793    1687    2866    3368    3573 
   Size:         92     701     894    1179     502     205

  Fragments arranged by size: 

                1179     894     701     502     205      92
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     676    1672    1852    2977    3573 
   Size:        676     996     180    1125     596

  Fragments arranged by size: 

                1125     996     676     596     180
 
EcoICRI GAG'CTC

Cuts at:      0    2661    3573 
   Size:       2661     912
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34      81     320     511     640     886     913    1036 
   Size:         34      47     239     191     129     246      27     123

Cuts at:   1036    1231    1384    1450    1668    1756    1960    2141    2365 
   Size:        195     153      66     218      88     204     181     224

Cuts at:   2365    2410    2847    3012    3229    3320    3436    3486    3573 
   Size:         45     437     165     217      91     116      50      87

  Fragments arranged by size: 

                 437     246     239     224     218     217     204     195
                 191     181     165     153     129     123     116      91
                  88      87      66      50      47      45      34      27
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     198    1051    2017    2137    2162    2845    3573 
   Size:        198     853     966     120      25     683     728

  Fragments arranged by size: 

                 966     853     728     683     198     120      25
 
EcoO109I rG'GnC_Cy

Cuts at:      0       8     132     139     292     387    1134    1221    1341 
   Size:          8     124       7     153      95     747      87     120

Cuts at:   1341    1986    2307    2646    2647    3281    3329    3537    3549 
   Size:        645     321     339       1     634      48     208      12

Cuts at:   3549    3573 
   Size:         24

  Fragments arranged by size: 

                 747     645     634     339     321     208     153     124
                 120      95      87      48      24      12       8       7
                   1
 
EcoRI G'AATT_C

Cuts at:      0    1618    3573 
   Size:       1618    1955
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2934    3573 
   Size:       2934     639
 
FspI TGC'GCA

Cuts at:      0    2806    3109    3573 
   Size:       2806     303     464

  Fragments arranged by size: 

                2806     464     303
 
FspAI rTGC'GCAy

Cuts at:      0    2806    3109    3573 
   Size:       2806     303     464

  Fragments arranged by size: 

                2806     464     303
 
HaeII r_GCGC'y

Cuts at:      0    2817    2916    3573 
   Size:       2817      99     657

  Fragments arranged by size: 

                2817     657      99
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     454     486     500     532    1815    1847    2554    2586 
   Size:        454      32      14      32    1283      32     707      32

Cuts at:   2586    3109    3141    3295    3327    3460    3492    3573 
   Size:        523      32     154      32     133      32      81

  Fragments arranged by size: 

                1283     707     523     454     154     133      81      32
                  32      32      32      32      32      32      14
 
HincII GTy'rAC

Cuts at:      0    2757    2895    3198    3573 
   Size:       2757     138     303     375

  Fragments arranged by size: 

                2757     375     303     138
 
HindIII A'AGCT_T

Cuts at:      0    1924    3573 
   Size:       1924    1649
 
HpaI GTT'AAC

Cuts at:      0    2757    3573 
   Size:       2757     816
 
KasI G'GCGC_C

Cuts at:      0    2813    3573 
   Size:       2813     760
 
MscI TGG'CCA

Cuts at:      0     571     583    1422    1837    2224    2728    3573 
   Size:        571      12     839     415     387     504     845

  Fragments arranged by size: 

                 845     839     571     504     415     387      12
 
MslI CAynn'nnrTG

Cuts at:      0    3013    3133    3573 
   Size:       3013     120     440

  Fragments arranged by size: 

                3013     440     120
 
MspA1I CmG'CkG

Cuts at:      0     223     891    1116    1545    1611    1641    1650    1770 
   Size:        223     668     225     429      66      30       9     120

Cuts at:   1770    1776    1877    1993    2202    2315    2703    2778    3058 
   Size:          6     101     116     209     113     388      75     280

Cuts at:   3058    3377    3521    3573 
   Size:        319     144      52

  Fragments arranged by size: 

                 668     429     388     319     280     225     223     209
                 144     120     116     113     101      75      66      52
                  30       9       6
 
NarI GG'CG_CC

Cuts at:      0    2814    3573 
   Size:       2814     759
 
NcoI C'CATG_G

Cuts at:      0    1091    1162    2729    2877    3128    3573 
   Size:       1091      71    1567     148     251     445

  Fragments arranged by size: 

                1567    1091     445     251     148      71
 
NheI G'CTAG_C

Cuts at:      0    1966    3573 
   Size:       1966    1607
 
NspI r_CATG'y

Cuts at:      0    1461    3573 
   Size:       1461    2112
 
PasI CC'CwG_GG

Cuts at:      0     367     427     823    1579    2304    3540    3573 
   Size:        367      60     396     756     725    1236      33

  Fragments arranged by size: 

                1236     756     725     396     367      60      33
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      59    1092    1362    1653    2119    2598    3573 
   Size:         59    1033     270     291     466     479     975

  Fragments arranged by size: 

                1033     975     479     466     291     270      59
 
PfoI T'CCnGG_A

Cuts at:      0     199    3297    3573 
   Size:        199    3098     276

  Fragments arranged by size: 

                3098     276     199
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2968    3000    3573 
   Size:       2968      32     573

  Fragments arranged by size: 

                2968     573      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     132     139     387    1134    1221    1986    3329    3573 
   Size:        132       7     248     747      87     765    1343     244

  Fragments arranged by size: 

                1343     765     747     248     244     132      87       7
            
 
PshAI GACnn'nnGTC

Cuts at:      0     137     392    1132    2936    3573 
   Size:        137     255     740    1804     637

  Fragments arranged by size: 

                1804     740     637     255     137
 
PspOMI G'GGCC_C

Cuts at:      0    2646    2881    3573 
   Size:       2646     235     692

  Fragments arranged by size: 

                2646     692     235
 
PstI C_TGCA'G

Cuts at:      0     228     890    1322    1409    1541    1775    2624    3023 
   Size:        228     662     432      87     132     234     849     399

Cuts at:   3023    3573 
   Size:        550

  Fragments arranged by size: 

                 849     662     550     432     399     234     228     132
                  87
 
PvuI CG_AT'CG

Cuts at:      0    2037    3573 
   Size:       2037    1536
 
PvuII CAG'CTG

Cuts at:      0     223     891    1116    1611    1770    1776    2202    2703 
   Size:        223     668     225     495     159       6     426     501

Cuts at:   2703    2778    3058    3521    3573 
   Size:         75     280     463      52

  Fragments arranged by size: 

                 668     501     495     463     426     280     225     223
                 159      75      52       6
 
SacI G_AGCT'C

Cuts at:      0    2663    3573 
   Size:       2663     910
 
SacII CC_GC'GG

Cuts at:      0    2316    3573 
   Size:       2316    1257
 
SanDI GG'GwC_CC

Cuts at:      0     387    3329    3573 
   Size:        387    2942     244

  Fragments arranged by size: 

                2942     387     244
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1687    2866    3368    3573 
   Size:       1687    1179     502     205

  Fragments arranged by size: 

                1687    1179     502     205
 
SexAI A'CCwGG_T

Cuts at:      0     836    1496    3573 
   Size:        836     660    2077

  Fragments arranged by size: 

                2077     836     660
 
SfcI C'TryA_G

Cuts at:      0     181     224     886    1021    1120    1248    1318    1405 
   Size:        181      43     662     135      99     128      70      87

Cuts at:   1405    1537    1771    2025    2197    2469    2620    3019    3573 
   Size:        132     234     254     172     272     151     399     554

  Fragments arranged by size: 

                 662     554     399     272     254     234     181     172
                 151     135     132     128      99      87      70      43
            
 
SfoI GGC'GCC

Cuts at:      0    2815    3573 
   Size:       2815     758
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1950    3573 
   Size:       1950    1623
 
SmaI CCC'GGG

Cuts at:      0    1372    1751    3573 
   Size:       1372     379    1822

  Fragments arranged by size: 

                1822    1372     379
 
SmlI C'TyrA_G

Cuts at:      0     420    1927    2761    3573 
   Size:        420    1507     834     812

  Fragments arranged by size: 

                1507     834     812     420
 
StuI AGG'CCT

Cuts at:      0     965     985    1693    2023    3025    3049    3148    3573 
   Size:        965      20     708     330    1002      24      99     425

  Fragments arranged by size: 

                1002     965     708     425     330      99      24      20
            
 
StyI C'CwwG_G

Cuts at:      0     456     609    1091    1162    1473    2729    2877    2934 
   Size:        456     153     482      71     311    1256     148      57

Cuts at:   2934    3128    3573 
   Size:        194     445

  Fragments arranged by size: 

                1256     482     456     445     311     194     153     148
                  71      57
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     181    1099    2423    3121    3573 
   Size:        181     918    1324     698     452

  Fragments arranged by size: 

                1324     918     698     452     181
 
TatI w'GTAC_w

Cuts at:      0     726    2255    3347    3573 
   Size:        726    1529    1092     226

  Fragments arranged by size: 

                1529    1092     726     226
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     731     763    3573 
   Size:        731      32    2810

  Fragments arranged by size: 

                2810     731      32
 
XbaI T'CTAG_A

Cuts at:      0    2250    3573 
   Size:       2250    1323
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1169    2595    3573 
   Size:       1169    1426     978

  Fragments arranged by size: 

                1426    1169     978
 
XmaI C'CCGG_G

Cuts at:      0    1370    1749    3573 
   Size:       1370     379    1824

  Fragments arranged by size: 

                1824    1370     379
 
XmnI GAAnn'nnTTC

Cuts at:      0    2272    3573 
   Size:       2272    1301
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AgeI     AleI     AlfI    AlwNI     ApaI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     MscI     MslI   MspA1I     NarI     NcoI     NheI     NspI
     PasI    PflMI     PfoI     PpiI    PpuMI    PshAI   PspOMI     PstI
     PvuI    PvuII     SacI    SacII    SanDI     SapI    SexAI     SfcI
     SfoI    SgrAI     SmaI     SmlI     StuI     StyI    TaqII     TatI
     TstI     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AhdI     AloI
     AscI     AseI    AsiSI    AvrII     BaeI     BbsI     BclI    BfrBI
     BplI    BsaAI    BsiWI     BsmI    BsmBI    BspEI    BspHI   BssHII
    BstBI  BstZ17I     ClaI     DraI     EagI    EcoRV     FseI     KpnI
     MfeI     MluI     MmeI     NaeI     NdeI   NgoMIV     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PsiI    PspXI     PsrI
    RsrII     SalI     SbfI     ScaI     SfiI     SgfI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI  Tth111I     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com