Restriction map for pF1KB9874
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AvaII       AviII       BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BlpI        Bpu1102I    BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstXI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraII       EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoRV       FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NspI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SalI        
SapI        SbfI        SexAI       SfaNI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SspBI       SwaI        Tth111I     XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI          271  GTCTAC               GT^[AC][GT]AC
AccII        1256  CGCG                 CG^CG
AlwI          381  GGATC                GGATC(4/5)
AlwNI        1452  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         804  GAGCAC               G[AT]GC[AT]^C
BalI          570  TGGCCA               TGG^CCA
BbsI          255  GTCTTC               GAAGAC(2/6)
BcnI         1302  CCGGG                CC^[GC]GG
BpuAI         255  GTCTTC               GAAGAC(2/6)
BsaAI        1486  CACGTA               [CT]AC^GT[GA]
BsaBI         847  GATTATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI       804  GAGCAC               G[AT]GC[AT]^C
BsmAI         604  GTCTC                GTCTC(1/5)
BsmI           98  GAATGC               GAATGC(1/-1)
BspHI         512  TCATGA               T^CATGA
BspMI         240  GCAGGT               ACCTGC(4/8)
BsrBI        1161  CCGCTC               CCGCTC(-3/-3)
BstBI          95  TTCGAA               TT^CGAA
BstUI        1256  CGCG                 CG^CG
Cac8I        1233  GCCAGC               GC[ACGT]^[ACGT]GC
DraIII       1486  CACGTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          595  GACTGTTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          570  TGGCCA               [CT]^GGCC[GA]
EarI          313  GAAGAG               CTCTTC(1/4)
EcoRI         760  GAATTC               G^AATTC
FauI         1521  GCGGG                CCCGC(4/6)
HapII        1302  CCGG                 C^CGG
HpaII        1302  CCGG                 C^CGG
Ksp632I       313  GAAGAG               CTCTTC(1/4)
KspI         1255  CCGCGG               CCGC^GG
MaeII        1487  ACGT                 A^CGT
MamI          847  GATTATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         570  TGGCCA               TGG^CCA
MscI          570  TGGCCA               TGG^CCA
MspI         1302  CCGG                 C^CGG
MvnI         1256  CGCG                 CG^CG
NciI         1302  CCGGG                CC^[GC]GG
NcoI          567  CCATGG               C^CATGG
NspV           95  TTCGAA               TT^CGAA
PflMI        1095  CCAGTTAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          830  GACTC                GAGTC(4/5)
RcaI          512  TCATGA               T^CATGA
SacII        1255  CCGCGG               CCGC^GG
ScaI         1009  AGTACT               AGT^ACT
SfuI           95  TTCGAA               TT^CGAA
SpeI         1012  ACTAGT               A^CTAGT
StuI          203  AGGCCT               AGG^CCT
Van91I       1095  CCAGTTAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1150  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AvrII         376  CCTAGG               C^CTAGG
              696  CCTAGG              
BbvI          118  GCAGC                GCAGC(8/12)
              179  GCTGC               
BglII          68  AGATCT               A^GATCT
             1533  AGATCT              
BlnI          376  CCTAGG               C^CTAGG
              696  CCTAGG              
BmyI          804  GAGCAC               G[AGT]GC[ACT]^C
             1347  GGGCAC              
BseRI         355  GAGGAG               GAGGAG(10/8)
              387  GAGGAG              
BsmFI           5  GGGAC                GGGAC(10/14)
             1494  GTCCC               
Bsp1286I      804  GAGCAC               G[AGT]GC[ACT]^C
             1347  GGGCAC              
BstYI          68  AGATCT               [GA]^GATC[CT]
             1533  AGATCT              
Cfr13I        279  GGGCC                G^G[ACGT]CC
             1332  GGGCC               
DraI          703  TTTAAA               TTT^AAA
              897  TTTAAA              
DsaI          567  CCATGG               C^C[GA][CT]GG
             1255  CCGCGG              
EcoNI        1312  CCTCCTAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1369  CCTTCACAAGG         
EcoT22I       284  ATGCAT               ATGCA^T
              957  ATGCAT              
MflI           68  AGATCT               [GA]^GATC[CT]
             1533  AGATCT              
MslI          465  CACTTATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              879  CACTGCAGTG          
NsiI          284  ATGCAT               ATGCA^T
              957  ATGCAT              
PvuII         119  CAGCTG               CAG^CTG
              412  CAGCTG              
Sau96I        279  GGGCC                G^G[ACGT]CC
             1332  GGGCC               
SspI          627  AATATT               AAT^ATT
             1068  AATATT              
TaqI           96  TCGA                 T^CGA
              593  TCGA                
TseI          118  GCAGC                G^C[AT]GC
              179  GCTGC               
TthHB8I        96  TCGA                 T^CGA
              593  TCGA                
XhoII          68  AGATCT               [GA]^GATC[CT]
             1533  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BfaI        BpmI        BsaJI       BsiYI       BslI        
BsrI        BssKI       BstEII      BstF5I      BstNI       
BstPI       DdeI        DpnII       EcoO65I     EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       PstI        
RsaI        Sau3AI      ScrFI       SfcI        SmlI        
StyI        TfiI        Tru9I       Tsp45I      Tsp509I     
TspRI       
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