Restriction map for pF1KB9868
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AflIII      
AgeI        AhdI        AlwNI       AocI        AscI        
AseI        AsnI        Asp718I     AspEI       AvrII       
BamHI       BbrPI       BbvCI       BciVI       BclI        
BfaI        BfrI        BlnI        BlpI        Bpu1102I    
BsaBI       BsaI        BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspLU11I    BsrDI       BsrGI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DdeI        DraI        DrdI        Eam1105I    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        HincII      HindII      HindIII     HinfI       
HpaI        KpnI        MaeI        MamI        MfeI        
MluI        MseI        MunI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SalI        ScaI        SexAI       SfuI        
SgrAI       SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
TspRI       Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          643  GTCTAC               GT^[AC][GT]AC
AccIII        705  TCCGGA               T^CCGGA
AcsI         1225  AAATTC               [GA]^AATT[CT]
Alw44I        364  GTGCAC               G^TGCAC
Aor51HI      1109  AGCGCT               AGC^GCT
ApaLI         364  GTGCAC               G^TGCAC
ApoI         1225  AAATTC               [GA]^AATT[CT]
Asp700I      1107  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          709  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         650  TGCGCA               TGC^GCA
BbsI          891  GTCTTC               GAAGAC(2/6)
BcgI          149  GCAACGTGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         320  AGATCT               A^GATCT
BpuAI         891  GTCTTC               GAAGAC(2/6)
BsaAI         646  TACGTG               [CT]AC^GT[GA]
BsaWI         705  TCCGGA               [AT]^CCGG[AT]
BseAI         705  TCCGGA               T^CCGGA
BsgI          348  CTGCAC               GTGCAG(16/14)
BsmAI         219  GTCTC                GTCTC(1/5)
BspEI         705  TCCGGA               T^CCGGA
BspHI         632  TCATGA               T^CATGA
BspMI          90  GCAGGT               ACCTGC(4/8)
BssHII        185  GCGCGC               G^CGCGC
BssSI         177  CACGAG               CACGAG(-5/-1)
DraIII        465  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     1109  AGCGCT               AGC^GCT
EcoT14I      1070  CCAAGG               C^C[AT][AT]GG
FseI          117  GGCCGGCC             GGCCGG^CC
FspI          650  TGCGCA               TGC^GCA
KspI          997  CCGCGG               CCGC^GG
MaeIII         94  GTGAC                ^GT[ACGT]AC
MroI          705  TCCGGA               T^CCGGA
PflFI         709  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI          632  TCATGA               T^CATGA
SacI          885  GAGCTC               GAGCT^C
SacII         997  CCGCGG               CCGC^GG
SbfI           87  CCTGCAGG             CCTGCA^GG
SfiI          911  GGCCTCCAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          190  CTCAAG               C^T[CT][GA]AG
Sse8387I       87  CCTGCAGG             CCTGCA^GG
StyI         1070  CCAAGG               C^C[AT][AT]GG
Tsp45I         94  GTGAC                ^GT[GC]AC
Tsp509I      1226  AATT                 ^AATT
Tth111I       709  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          275  CCAACGAGGTCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1107  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BalI          245  TGGCCA               TGG^CCA
              272  TGGCCA              
BpmI          914  CTCCAG               CTGGAG(16/14)
              877  CTGGAG              
BseRI         880  GAGGAG               GAGGAG(10/8)
             1051  GAGGAG              
BsrBI         502  CCGCTC               CCGCTC(-3/-3)
              907  GAGCGG              
BsrI          293  ACTGG                ACTGG(1/-1)
              395  ACTGG               
BstF5I        237  CATCC                GGATG(2/0)
              258  CATCC               
BstXI         377  CCATCAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              749  CCAACGGTCTGG        
BstYI         320  AGATCT               [GA]^GATC[CT]
             1316  AGATCC              
DsaI          997  CCGCGG               C^C[GA][CT]GG
             1130  CCGTGG              
FokI          237  CATCC                GGATG(9/13)
              258  CATCC               
MflI          320  AGATCT               [GA]^GATC[CT]
             1316  AGATCC              
MluNI         245  TGGCCA               TGG^CCA
              272  TGGCCA              
MscI          245  TGGCCA               TGG^CCA
              272  TGGCCA              
MspA1I        802  CCGCTG               C[AC]G^C[GT]G
              997  CCGCGG              
SapI          342  GCTCTTC              GCTCTTC(1/4)
             1218  GCTCTTC             
XhoII         320  AGATCT               [GA]^GATC[CT]
             1316  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        AspHI       AvaI        AvaII       
BanI        BanII       BbeI        BbvI        BcnI        
BglI        BmyI        BsaHI       BsaJI       BsiEI       
BsiHKAI     BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BsrFI       BssKI       BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DpnII       
DraII       EaeI        EagI        EarI        EclXI       
Eco52I      EcoO109I    EcoRII      FauI        Fnu4HI      
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HpaII       HphI        ItaI        
KasI        Ksp632I     MaeII       MboI        MboII       
MnlI        MslI        MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmaI        TaqI        TseI        TthHB8I     
XmaCI       XmaI        
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