Restriction map for pF1KB9864
 (Linear) (Six-base) MAPSORT of: KIBB9864.seq  Check: 5643  from: 1  to: 1266



KIBB9864.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AfeI AGC'GCT

Cuts at:      0     193    1266 
   Size:        193    1073
 
AflIII A'CryG_T

Cuts at:      0    1226    1266 
   Size:       1226      40
 
AlwNI CAG_nnn'CTG

Cuts at:      0     404     785    1266 
   Size:        404     381     481

  Fragments arranged by size: 

                 481     404     381
 
ApaLI G'TGCA_C

Cuts at:      0     883    1266 
   Size:        883     383
 
AscI GG'CGCG_CC

Cuts at:      0     453    1266 
   Size:        453     813
 
AvaI C'yCGr_G

Cuts at:      0    1013    1266 
   Size:       1013     253
 
BanI G'GyrC_C

Cuts at:      0     859    1054    1144    1266 
   Size:        859     195      90     122

  Fragments arranged by size: 

                 859     195     122      90
 
BbeI G_GCGC'C

Cuts at:      0     863    1148    1266 
   Size:        863     285     118

  Fragments arranged by size: 

                 863     285     118
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     542     709    1030    1039    1266 
   Size:        542     167     321       9     227

  Fragments arranged by size: 

                 542     321     227     167       9
 
BclI T'GATC_A

Cuts at:      0     110    1266 
   Size:        110    1156
 
BlpI GC'TnA_GC

Cuts at:      0      10    1266 
   Size:         10    1256
 
Bme1580I G_kGCm'C

Cuts at:      0     133     887     951    1057    1266 
   Size:        133     754      64     106     209

  Fragments arranged by size: 

                 754     209     133     106      64
 
BmgBI CAC'GTC

Cuts at:      0      90     348    1266 
   Size:         90     258     918

  Fragments arranged by size: 

                 918     258      90
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     319     414    1266 
   Size:        319      95     852

  Fragments arranged by size: 

                 852     319      95
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     807     889    1266 
   Size:        807      82     377

  Fragments arranged by size: 

                 807     377      82
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      52    1266 
   Size:         52    1214
 
BsaAI yAC'GTr

Cuts at:      0     432    1266 
   Size:        432     834
 
BsaHI Gr'CG_yC

Cuts at:      0     445     860    1145    1266 
   Size:        445     415     285     121

  Fragments arranged by size: 

                 445     415     285     121
 
BsaWI w'CCGG_w

Cuts at:      0     706    1266 
   Size:        706     560
 
BseYI C'CCAG_C

Cuts at:      0     126     153     516     792     811     922    1038    1161 
   Size:        126      27     363     276      19     111     116     123

Cuts at:   1161    1266 
   Size:        105

  Fragments arranged by size: 

                 363     276     126     123     116     111     105      27
                  19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     342    1266 
   Size:        342     924
 
BsiHKAI G_wGCw'C

Cuts at:      0      11     296     822     887     911    1266 
   Size:         11     285     526      65      24     355

  Fragments arranged by size: 

                 526     355     285      65      24      11
 
Bsp1286I G_dGCh'C

Cuts at:      0      11     133     296     822     887     911     951    1057 
   Size:         11     122     163     526      65      24      40     106

Cuts at:   1057    1266 
   Size:        209

  Fragments arranged by size: 

                 526     209     163     122     106      65      40      24
                  11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     331    1266 
   Size:        331     935
 
BsrBI CCG'CTC

Cuts at:      0     542     712    1266 
   Size:        542     170     554

  Fragments arranged by size: 

                 554     542     170
 
BsrDI GCAATG_nn'

Cuts at:      0     165    1266 
   Size:        165    1101
 
BsrFI r'CCGG_y

Cuts at:      0      45      61    1266 
   Size:         45      16    1205

  Fragments arranged by size: 

                1205      45      16
 
BssHII G'CGCG_C

Cuts at:      0     453     664    1266 
   Size:        453     211     602

  Fragments arranged by size: 

                 602     453     211
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     164    1266 
   Size:        164    1102
 
BstEII G'GTnAC_C

Cuts at:      0     237     318    1266 
   Size:        237      81     948

  Fragments arranged by size: 

                 948     237      81
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     186     393    1140    1266 
   Size:        186     207     747     126

  Fragments arranged by size: 

                 747     207     186     126
 
BtgI C'CryG_G

Cuts at:      0     272     723    1266 
   Size:        272     451     543

  Fragments arranged by size: 

                 543     451     272
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     165     321     372    1266 
   Size:        165     156      51     894

  Fragments arranged by size: 

                 894     165     156      51
 
DraIII CAC_nnn'GTG

Cuts at:      0     891    1266 
   Size:        891     375
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      94    1266 
   Size:         94    1172
 
EaeI y'GGCC_r

Cuts at:      0     209     275    1266 
   Size:        209      66     991

  Fragments arranged by size: 

                 991     209      66
 
EarI CTCTTCn'nnn_

Cuts at:      0     748    1266 
   Size:        748     518
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     807     889    1266 
   Size:        807      82     377

  Fragments arranged by size: 

                 807     377      82
 
HaeII r_GCGC'y

Cuts at:      0     195     863    1148    1266 
   Size:        195     668     285     118

  Fragments arranged by size: 

                 668     285     195     118
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     102     134    1266 
   Size:        102      32    1132

  Fragments arranged by size: 

                1132     102      32
 
KasI G'GCGC_C

Cuts at:      0     859    1144    1266 
   Size:        859     285     122

  Fragments arranged by size: 

                 859     285     122
 
MfeI C'AATT_G

Cuts at:      0    1155    1266 
   Size:       1155     111
 
MluI A'CGCG_T

Cuts at:      0    1226    1266 
   Size:       1226      40
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     110    1196    1266 
   Size:        110    1086      70

  Fragments arranged by size: 

                1086     110      70
 
MscI TGG'CCA

Cuts at:      0     211    1266 
   Size:        211    1055
 
MslI CAynn'nnrTG

Cuts at:      0    1048    1266 
   Size:       1048     218
 
MspA1I CmG'CkG

Cuts at:      0     460     621    1121    1266 
   Size:        460     161     500     145

  Fragments arranged by size: 

                 500     460     161     145
 
NarI GG'CG_CC

Cuts at:      0     860    1145    1266 
   Size:        860     285     121

  Fragments arranged by size: 

                 860     285     121
 
NcoI C'CATG_G

Cuts at:      0     272     723    1266 
   Size:        272     451     543

  Fragments arranged by size: 

                 543     451     272
 
NspI r_CATG'y

Cuts at:      0     560    1131    1266 
   Size:        560     571     135

  Fragments arranged by size: 

                 571     560     135
 
PstI C_TGCA'G

Cuts at:      0     203    1266 
   Size:        203    1063
 
PvuII CAG'CTG

Cuts at:      0    1121    1266 
   Size:       1121     145
 
SfcI C'TryA_G

Cuts at:      0     199    1266 
   Size:        199    1067
 
SfoI GGC'GCC

Cuts at:      0     861    1146    1266 
   Size:        861     285     120

  Fragments arranged by size: 

                 861     285     120
 
SphI G_CATG'C

Cuts at:      0     560    1266 
   Size:        560     706
 
StyI C'CwwG_G

Cuts at:      0     185     272     723    1266 
   Size:        185      87     451     543

  Fragments arranged by size: 

                 543     451     185      87
 

 Enzymes that do cut:

     AfeI   AflIII    AlwNI    ApaLI     AscI     AvaI     BanI     BbeI
     BbsI     BclI     BlpI Bme1580I    BmgBI     BmrI     BpmI     BsaI
    BsaAI    BsaHI    BsaWI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
    BsrBI    BsrDI    BsrFI   BssHII   BstAPI   BstEII    BstXI     BtgI
    BtgZI   DraIII     DrdI     EaeI     EarI  Eco57MI    HaeII    Hin4I
     KasI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NarI
     NcoI     NspI     PstI    PvuII     SfcI     SfoI     SphI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI     ApoI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII     BmtI     BplI   Bpu10I    BpuEI    BsaBI    BsaXI
    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrGI
    BssSI    BstBI    BstYI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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