Restriction map for pF1KB9858
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AflII       AflIII      AgeI        Alw44I      
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AvaI        
AviII       AvrII       BbrPI       BbsI        BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BpuAI       BsaAI       BsaBI       BsiEI       BsiWI       
BsmI        BsoBI       BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     ClaI        CpoI        DraI        
DrdI        EagI        EarI        EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        Ksp632I     KspI        
MaeII       MamI        MboII       MfeI        MluI        
MseI        MunI        MvnI        NdeI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfcI        SfiI        SfuI        SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspI        StuI        
SwaI        TaqI        TfiI        Tru9I       Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AfaI          132  GTAC                 GT^AC
AhdI          659  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI          857  GGGCCC               GGGCC^C
AspEI         659  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI          830  GGCGCC               GGCGC^C
BbvCI         185  CCTCAGC              CCTCAGC(-5/-2)
BcnI          965  CCGGG                CC^[GC]GG
BglI          787  GCCCTGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          867  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      867  GCTGAGC              GC^T[ACGT]AGC
BsgI           70  GTGCAG               GTGCAG(16/14)
BsmBI        1184  GAGACG               CGTCTC(1/5)
Bsp1407I      131  TGTACA               T^GTACA
BsrFI         833  GCCGGC               [GA]^CCGG[CT]
BsrGI         131  TGTACA               T^GTACA
CelII         867  GCTGAGC              GC^T[ACGT]AGC
CfoI          831  GCGC                 GCG^C
Cfr10I        833  GCCGGC               [GA]^CCGG[CT]
DraIII        382  CACACCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      659  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         425  CCTCCAGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          647  GCGGG                CCCGC(4/6)
HaeII         830  GGCGCC               [GA]GCGC^[CT]
HhaI          831  GCGC                 GCG^C
HinP1I        831  GCGC                 G^CGC
HinfI         574  GACTC                G^A[ACGT]TC
HphI         1025  TCACC                GGTGA(8/7)
KasI          830  GGCGCC               G^GCGCC
MslI          297  CATCAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        645  CAGCGG               C[AC]G^C[GT]G
NaeI          833  GCCGGC               GCC^GGC
NarI          830  GGCGCC               GG^CGCC
NciI          965  CCGGG                CC^[GC]GG
NgoMIV        833  GCCGGC               G^CCGGC
NheI          463  GCTAGC               G^CTAGC
NspI          761  GCATGC               [GA]CATG^[CT]
PleI          574  GACTC                GAGTC(4/5)
PpuMI         923  AGGTCCC              [GA]G^G[AT]CC[CT]
PshAI        1043  GACGGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI          132  GTAC                 GT^AC
SacI          120  GAGCTC               GAGCT^C
SfoI          830  GGCGCC               GGC^GCC
SgrAI         832  CGCCGGCG             C[GA]^CCGG[CT]G
SphI          761  GCATGC               GCATG^C
SspBI         131  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccIII         85  TCCGGA               T^CCGGA
              734  TCCGGA              
AlwNI         770  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1160  CAGCTCCTG           
AocI          157  CCTAAGG              CC^T[ACGT]AGG
              928  CCTGAGG             
AspHI          57  GAGCAC               G[AT]GC[AT]^C
              120  GAGCTC              
BalI            2  TGGCCA               TGG^CCA
             1130  TGGCCA              
BamHI         220  GGATCC               G^GATCC
              376  GGATCC              
BsaWI          85  TCCGGA               [AT]^CCGG[AT]
              734  TCCGGA              
BseAI          85  TCCGGA               T^CCGGA
              734  TCCGGA              
BsiHKAI        57  GAGCAC               G[AT]GC[AT]^C
              120  GAGCTC              
BspEI          85  TCCGGA               T^CCGGA
              734  TCCGGA              
BstF5I        343  CATCC                GGATG(2/0)
             1140  GGATG               
BstYI         220  GGATCC               [GA]^GATC[CT]
              376  GGATCC              
Bsu36I        157  CCTAAGG              CC^T[ACGT]AGG
              928  CCTGAGG             
DpnII         221  GATC                 ^GATC
              377  GATC                
EaeI            2  TGGCCA               [CT]^GGCC[GA]
             1130  TGGCCA              
Eco81I        157  CCTAAGG              CC^T[ACGT]AGG
              928  CCTGAGG             
FokI          343  CATCC                GGATG(9/13)
             1140  GGATG               
MboI          221  GATC                 ^GATC
              377  GATC                
MflI          220  GGATCC               [GA]^GATC[CT]
              376  GGATCC              
MluNI           2  TGGCCA               TGG^CCA
             1130  TGGCCA              
MroI           85  TCCGGA               T^CCGGA
              734  TCCGGA              
MscI            2  TGGCCA               TGG^CCA
             1130  TGGCCA              
NcoI          509  CCATGG               C^CATGG
              563  CCATGG              
NdeII         221  GATC                 ^GATC
              377  GATC                
Sau3AI        221  GATC                 ^GATC
              377  GATC                
XhoII         220  GGATCC               [GA]^GATC[CT]
              376  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AciI        AcyI        AluI        AlwI        AvaII       
BanI        BanII       BbvI        BfaI        BmyI        
BpmI        BsaHI       BsaI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BssSI       BstNI       Cac8I       
Cfr13I      DdeI        DraII       DsaI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      HaeIII      HapII       
HgaI        Hin1I       HpaII       ItaI        MaeI        
MaeIII      MnlI        MspI        MvaI        MwoI        
NlaIII      NlaIV       Sau96I      ScrFI       StyI        
TseI        Tsp45I      TspRI       
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