Restriction map for pF1KB9857
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

Acc65I      AccII       AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BbrPI       
BbvCI       BcgI        BciVI       BcnI        BfrI        
BglI        BglII       BlnI        BsaAI       BsgI        
BsiEI       BsiWI       BsmI        Bsp1407I    BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     Cfr10I      CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoRI       EcoT22I     FseI        FspI        
HindIII     HpaI        KpnI        KspI        MfeI        
MluI        MluNI       MscI        MunI        MvnI        
NaeI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AatII         309  GACGTC               GACGT^C
AccI          280  GTAGAC               GT^[AC][GT]AC
AccIII       1004  TCCGGA               T^CCGGA
AfaI           15  GTAC                 GT^AC
AlwNI         541  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1084  CCTGAGG              CC^T[ACGT]AGG
BbeI          784  GGCGCC               GGCGC^C
BbsI         1134  GTCTTC               GAAGAC(2/6)
BclI            5  TGATCA               T^GATCA
BlpI          513  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      513  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1134  GTCTTC               GAAGAC(2/6)
BsaBI         132  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          529  GAGACC               GGTCTC(1/5)
BsaWI        1004  TCCGGA               [AT]^CCGG[AT]
BseAI        1004  TCCGGA               T^CCGGA
BseRI         157  CTCCTC               GAGGAG(10/8)
BsmBI         595  CGTCTC               CGTCTC(1/5)
BspDI        1024  ATCGAT               AT^CGAT
BspEI        1004  TCCGGA               T^CCGGA
BspHI         833  TCATGA               T^CATGA
BsrDI         294  CATTGC               GCAATG(2/0)
BssSI         275  CTCGTG               CACGAG(-5/-1)
BstEII         42  GGTCACC              G^GT[ACGT]ACC
BstPI          42  GGTCACC              G^GT[ACGT]ACC
BstYI         131  AGATCC               [GA]^GATC[CT]
Bsu36I       1084  CCTGAGG              CC^T[ACGT]AGG
CelII         513  GCTCAGC              GC^T[ACGT]AGC
ClaI         1024  ATCGAT               AT^CGAT
DsaI          974  CCATGG               C^C[GA][CT]GG
EaeI          897  TGGCCG               [CT]^GGCC[GA]
Eco81I       1084  CCTGAGG              CC^T[ACGT]AGG
EcoO65I        42  GGTCACC              G^GT[ACGT]ACC
EcoRV         208  GATATC               GAT^ATC
FbaI            5  TGATCA               T^GATCA
HaeII         784  GGCGCC               [GA]GCGC^[CT]
HgaI          385  GCGTC                GACGC(5/10)
HincII        775  GTTGAC               GT[CT]^[GA]AC
HindII        775  GTTGAC               GT[CT]^[GA]AC
KasI          784  GGCGCC               G^GCGCC
MaeII         310  ACGT                 A^CGT
MamI          132  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          131  AGATCC               [GA]^GATC[CT]
MroI         1004  TCCGGA               T^CCGGA
MseI          737  TTAA                 T^TAA
NarI          784  GGCGCC               GG^CGCC
NcoI          974  CCATGG               C^CATGG
NspI          443  GCATGC               [GA]CATG^[CT]
PstI          223  CTGCAG               CTGCA^G
RcaI          833  TCATGA               T^CATGA
RsaI           15  GTAC                 GT^AC
SacI           76  GAGCTC               GAGCT^C
SfcI          223  CTGCAG               C^T[GA][CT]AG
SfoI          784  GGCGCC               GGC^GCC
SphI          443  GCATGC               GCATG^C
Tru9I         737  TTAA                 T^TAA
XhoII         131  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AspHI          76  GAGCTC               G[AT]GC[AT]^C
              945  GTGCTC              
BpmI           55  CTCCAG               CTGGAG(16/14)
              251  CTCCAG              
BsiHKAI        76  GAGCTC               G[AT]GC[AT]^C
              945  GTGCTC              
BsrI          615  ACTGG                ACTGG(1/-1)
             1060  ACTGG               
CfoI          351  GCGC                 GCG^C
              785  GCGC                
EcoT14I       497  CCTTGG               C^C[AT][AT]GG
              974  CCATGG              
FauI          358  CCCGC                CCCGC(4/6)
              427  CCCGC               
HapII         654  CCGG                 C^CGG
             1005  CCGG                
HhaI          351  GCGC                 GCG^C
              785  GCGC                
HinP1I        351  GCGC                 G^CGC
              785  GCGC                
HpaII         654  CCGG                 C^CGG
             1005  CCGG                
MspI          654  CCGG                 C^CGG
             1005  CCGG                
SfaNI         726  GATGC                GCATC(5/9)
              756  GCATC               
StyI          497  CCTTGG               C^C[AT][AT]GG
              974  CCATGG              
TfiI          918  GATTC                G^A[AT]TC
             1168  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AcyI        AluI        AlwI        AvaI        
AvaII       BanI        BanII       BbvI        BfaI        
BmyI        BsaHI       BsaJI       BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EarI        EcoNI       EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      Hin1I       
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MnlI        MslI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       Sau3AI      Sau96I      
ScrFI       SmlI        StuI        TaqI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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