Restriction map for pF1KB9857
 (Linear) (Six-base) MAPSORT of: KIBB9857.seq  Check: 1048  from: 1  to: 1188



KIBB9857.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AatII G_ACGT'C

Cuts at:      0     313    1188 
   Size:        313     875
 
AccI GT'mk_AC

Cuts at:      0     281    1188 
   Size:        281     907
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     270    1188 
   Size:        270     918
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     316     463    1188 
   Size:        316     147     725

  Fragments arranged by size: 

                 725     316     147
 
AlwNI CAG_nnn'CTG

Cuts at:      0     546    1188 
   Size:        546     642
 
AvaI C'yCGr_G

Cuts at:      0     245     703     780    1188 
   Size:        245     458      77     408

  Fragments arranged by size: 

                 458     408     245      77
 
BanI G'GyrC_C

Cuts at:      0     300     784    1054    1188 
   Size:        300     484     270     134

  Fragments arranged by size: 

                 484     300     270     134
 
BanII G_rGCy'C

Cuts at:      0      80     187     252    1188 
   Size:         80     107      65     936

  Fragments arranged by size: 

                 936     107      80      65
 
BbeI G_GCGC'C

Cuts at:      0     788    1188 
   Size:        788     400
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1127    1188 
   Size:       1127      61
 
BclI T'GATC_A

Cuts at:      0       5    1188 
   Size:          5    1183
 
BlpI GC'TnA_GC

Cuts at:      0     514    1188 
   Size:        514     674
 
Bme1580I G_kGCm'C

Cuts at:      0     264     883    1188 
   Size:        264     619     305

  Fragments arranged by size: 

                 619     305     264
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     236    1188 
   Size:         40     196     952

  Fragments arranged by size: 

                 952     196      40
 
Bpu10I CC'TnA_GC

Cuts at:      0     629    1188 
   Size:        629     559
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     547     594     982    1188 
   Size:        463      84      47     388     206

  Fragments arranged by size: 

                 463     388     206      84      47
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     523    1188 
   Size:        523     665
 
BsaBI GATnn'nnATC

Cuts at:      0     136    1188 
   Size:        136    1052
 
BsaHI Gr'CG_yC

Cuts at:      0     310     385     785    1188 
   Size:        310      75     400     403

  Fragments arranged by size: 

                 403     400     310      75
 
BsaWI w'CCGG_w

Cuts at:      0    1004    1188 
   Size:       1004     184
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     383     413    1188 
   Size:        383      30     775

  Fragments arranged by size: 

                 775     383      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     148    1188 
   Size:        148    1040
 
BseYI C'CCAG_C

Cuts at:      0     365     859    1188 
   Size:        365     494     329

  Fragments arranged by size: 

                 494     365     329
 
BsiHKAI G_wGCw'C

Cuts at:      0      80     949    1188 
   Size:         80     869     239

  Fragments arranged by size: 

                 869     239      80
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     601    1188 
   Size:        601     587
 
Bsp1286I G_dGCh'C

Cuts at:      0      80     187     252     264     883     949    1188 
   Size:         80     107      65      12     619      66     239

  Fragments arranged by size: 

                 619     239     107      80      66      65      12
 
BspEI T'CCGG_A

Cuts at:      0    1004    1188 
   Size:       1004     184
 
BspHI T'CATG_A

Cuts at:      0     833    1188 
   Size:        833     355
 
BsrDI GCAATG_nn'

Cuts at:      0     293    1188 
   Size:        293     895
 
BssSI C'ACGA_G

Cuts at:      0     275    1188 
   Size:        275     913
 
BstEII G'GTnAC_C

Cuts at:      0      42    1188 
   Size:         42    1146
 
BstYI r'GATC_y

Cuts at:      0     131    1188 
   Size:        131    1057
 
Bsu36I CC'TnA_GG

Cuts at:      0    1085    1188 
   Size:       1085     103
 
BtgI C'CryG_G

Cuts at:      0     974    1188 
   Size:        974     214
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     837    1188 
   Size:        837     351
 
ClaI AT'CG_AT

Cuts at:      0    1025    1188 
   Size:       1025     163
 
EaeI y'GGCC_r

Cuts at:      0     897    1188 
   Size:        897     291
 
EarI CTCTTCn'nnn_

Cuts at:      0     122     638     689    1188 
   Size:        122     516      51     499

  Fragments arranged by size: 

                 516     499     122      51
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     517    1188 
   Size:        517     671
 
EcoICRI GAG'CTC

Cuts at:      0      78    1188 
   Size:         78    1110
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     236     270    1188 
   Size:         40     196      34     918

  Fragments arranged by size: 

                 918     196      40      34
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      37     163     478    1188 
   Size:         37     126     315     710

  Fragments arranged by size: 

                 710     315     126      37
 
EcoO109I rG'GnC_Cy

Cuts at:      0      30      93     241     699    1188 
   Size:         30      63     148     458     489

  Fragments arranged by size: 

                 489     458     148      63      30
 
EcoRV GAT'ATC

Cuts at:      0     210    1188 
   Size:        210     978
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     607    1188 
   Size:        607     581
 
HaeII r_GCGC'y

Cuts at:      0     788    1188 
   Size:        788     400
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     107     139     146     178     522     554     579     611 
   Size:        107      32       7      32     344      32      25      32

Cuts at:    611     815     847    1188 
   Size:        204      32     341

  Fragments arranged by size: 

                 344     341     204     107      32      32      32      32
                  32      25       7
 
HincII GTy'rAC

Cuts at:      0     777    1188 
   Size:        777     411
 
KasI G'GCGC_C

Cuts at:      0     784    1188 
   Size:        784     404
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     479    1188 
   Size:        479     709
 
MslI CAynn'nnrTG

Cuts at:      0     145     298     979    1188 
   Size:        145     153     681     209

  Fragments arranged by size: 

                 681     209     153     145
 
MspA1I CmG'CkG

Cuts at:      0     361     369     518    1188 
   Size:        361       8     149     670

  Fragments arranged by size: 

                 670     361     149       8
 
NarI GG'CG_CC

Cuts at:      0     785    1188 
   Size:        785     403
 
NcoI C'CATG_G

Cuts at:      0     974    1188 
   Size:        974     214
 
NspI r_CATG'y

Cuts at:      0     447    1188 
   Size:        447     741
 
PasI CC'CwG_GG

Cuts at:      0     180    1188 
   Size:        180    1008
 
PpuMI rG'GwC_Cy

Cuts at:      0      30      93     241    1188 
   Size:         30      63     148     947

  Fragments arranged by size: 

                 947     148      63      30
 
PstI C_TGCA'G

Cuts at:      0     227    1188 
   Size:        227     961
 
SacI G_AGCT'C

Cuts at:      0      80    1188 
   Size:         80    1108
 
SfcI C'TryA_G

Cuts at:      0     223    1188 
   Size:        223     965
 
SfoI GGC'GCC

Cuts at:      0     786    1188 
   Size:        786     402
 
SmlI C'TyrA_G

Cuts at:      0     478     526     573     961    1188 
   Size:        478      48      47     388     227

  Fragments arranged by size: 

                 478     388     227      48      47
 
SphI G_CATG'C

Cuts at:      0     447    1188 
   Size:        447     741
 
StuI AGG'CCT

Cuts at:      0     407     571     992    1188 
   Size:        407     164     421     196

  Fragments arranged by size: 

                 421     407     196     164
 
StyI C'CwwG_G

Cuts at:      0     497     974    1188 
   Size:        497     477     214

  Fragments arranged by size: 

                 497     477     214
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     863    1188 
   Size:        863     325
 
TatI w'GTAC_w

Cuts at:      0      14    1188 
   Size:         14    1174
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     383     415     454     486     838     870    1104    1136 
   Size:        383      32      39      32     352      32     234      32

Cuts at:   1136    1188 
   Size:         52

  Fragments arranged by size: 

                 383     352     234      52      39      32      32      32
                  32
 
ZraI GAC'GTC

Cuts at:      0     311    1188 
   Size:        311     877
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AlfI    AlwNI     AvaI     BanI    BanII
     BbeI     BbsI     BclI     BlpI Bme1580I     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BsrDI    BssSI   BstEII    BstYI
   Bsu36I     BtgI    BtgZI     ClaI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRV     FalI    HaeII    Hin4I   HincII
     KasI     MmeI     MslI   MspA1I     NarI     NcoI     NspI     PasI
    PpuMI     PstI     SacI     SfcI     SfoI     SmlI     SphI     StuI
     StyI    TaqII     TatI     TstI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII    BmgBI     BmrI     BmtI     BplI    BsaAI     BsgI    BsiEI
    BsiWI     BsmI    BspMI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI    BstXI  BstZ17I     BtsI    CspCI     DraI   DraIII     DrdI
     EagI    EcoRI     FseI     FspI    FspAI  HindIII     HpaI     KpnI
     MfeI     MluI     MscI     NaeI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com