Restriction map for pF1KB9856
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        AlwNI       AocI        
ApaI        ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlpI        BpmI        Bpu1102I    BsiWI       BsmBI       
BsmFI       BspDI       BspLU11I    BsrDI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraII       DraIII      DrdI        Eam1105I    Eco81I      
EcoNI       EcoO109I    EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        MfeI        
MluI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SalI        SapI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TaqI        Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI          628  GTCTAC               GT^[AC][GT]AC
AccIII        841  TCCGGA               T^CCGGA
Alw44I       1161  GTGCAC               G^TGCAC
Aor51HI       746  AGCGCT               AGC^GCT
ApaLI        1161  GTGCAC               G^TGCAC
AvrII         945  CCTAGG               C^CTAGG
BalI          182  TGGCCA               TGG^CCA
BbsI          523  GTCTTC               GAAGAC(2/6)
BglI          265  GCCTCTCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          945  CCTAGG               C^CTAGG
BpuAI         523  GTCTTC               GAAGAC(2/6)
BsaAI         694  TACGTA               [CT]AC^GT[GA]
BsaI           49  GAGACC               GGTCTC(1/5)
BseAI         841  TCCGGA               T^CCGGA
BsiEI         336  CGGCCG               CG[GA][CT]^CG
Bsp1407I      323  TGTACA               T^GTACA
BspEI         841  TCCGGA               T^CCGGA
BspHI         503  TCATGA               T^CATGA
BspMI         284  ACCTGC               ACCTGC(4/8)
BsrBI         705  CCGCTC               CCGCTC(-3/-3)
BsrGI         323  TGTACA               T^GTACA
BstYI         844  GGATCT               [GA]^GATC[CT]
DraI         1126  TTTAAA               TTT^AAA
EagI          336  CGGCCG               C^GGCCG
EarI          577  GAAGAG               CTCTTC(1/4)
EclXI         336  CGGCCG               C^GGCCG
Eco47III      746  AGCGCT               AGC^GCT
Eco52I        336  CGGCCG               C^GGCCG
Ksp632I       577  GAAGAG               CTCTTC(1/4)
KspI           38  CCGCGG               CCGC^GG
MflI          844  GGATCT               [GA]^GATC[CT]
MluNI         182  TGGCCA               TGG^CCA
MroI          841  TCCGGA               T^CCGGA
MscI          182  TGGCCA               TGG^CCA
PstI           96  CTGCAG               CTGCA^G
PvuII         763  CAGCTG               CAG^CTG
RcaI          503  TCATGA               T^CATGA
SacII          38  CCGCGG               CCGC^GG
SfcI           96  CTGCAG               C^T[GA][CT]AG
SmaI          781  CCCGGG               CCC^GGG
SmlI           62  CTCAAG               C^T[CT][GA]AG
SnaBI         694  TACGTA               TAC^GTA
SspBI         323  TGTACA               T^GTACA
TfiI          838  GAATC                G^A[AT]TC
XhoII         844  GGATCT               [GA]^GATC[CT]
XmaCI         781  CCCGGG               C^CCGGG
XmaI          781  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AluI          474  AGCT                 AG^CT
              764  AGCT                
AlwI          844  GGATC                GGATC(4/5)
              950  GGATC               
AspHI          14  GTGCTC               G[AT]GC[AT]^C
             1161  GTGCAC              
AvaI          755  CCCGAG               C^[CT]CG[GA]G
              781  CCCGGG              
BanII          42  GGGCTC               G[GA]GC[CT]^C
              758  GAGCCC              
BbvI           98  GCAGC                GCAGC(8/12)
              543  GCTGC               
BsaBI         306  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1008  GATGCCTATC          
BsaWI         224  ACCGGA               [AT]^CCGG[AT]
              841  TCCGGA              
BseRI         667  CTCCTC               GAGGAG(10/8)
              580  GAGGAG              
BsgI          238  CTGCAC               GTGCAG(16/14)
              412  CTGCAC              
BsiHKAI        14  GTGCTC               G[AT]GC[AT]^C
             1161  GTGCAC              
BsmAI          49  GAGAC                GTCTC(1/5)
              880  GTCTC               
BsmI          592  GAATGC               GAATGC(1/-1)
             1135  GCATTC              
BsoBI         755  CCCGAG               C^[CT]CG[GA]G
              781  CCCGGG              
BsrFI         334  ACCGGC               [GA]^CCGG[CT]
              739  ACCGGC              
BsrI          348  ACTGG                ACTGG(1/-1)
              443  ACTGG               
BstEII        276  GGTCACC              G^GT[ACGT]ACC
              798  GGTCACC             
BstPI         276  GGTCACC              G^GT[ACGT]ACC
              798  GGTCACC             
Cfr10I        334  ACCGGC               [GA]^CCGG[CT]
              739  ACCGGC              
EcoO65I       276  GGTCACC              G^GT[ACGT]ACC
              798  GGTCACC             
MamI          306  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1008  GATGCCTATC          
MslI          297  CATCCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              415  CACCTCTGTG          
ScaI          147  AGTACT               AGT^ACT
              467  AGTACT              
TseI           98  GCAGC                G^C[AT]GC
              543  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AvaII       
BcnI        BfaI        BmyI        BsaHI       BsaJI       
BsiYI       BslI        Bsp1286I    BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DsaI        EaeI        EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        Tru9I       Tsp45I      
TspRI       
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