Restriction map for pF1KB9852
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BclI        BcnI        BfrI        
BglI        BglII       BlnI        BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstUI       BstZ17I     Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EaeI        EagI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoRV       EcoT22I     FbaI        FokI        
FseI        FspI        HapII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcsI         1026  GAATTC               [GA]^AATT[CT]
AhdI          775  GACTGTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI           17  CCTTAGG              CC^T[ACGT]AGG
ApoI         1026  GAATTC               [GA]^AATT[CT]
Asp700I       878  GAAACAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         775  GACTGTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII         762  GAGCCC               G[GA]GC[CT]^C
BciVI         473  GTATCC               GTATCC(6/5)
BlpI          759  GCTGAGC              GC^T[ACGT]AGC
BmyI          762  GAGCCC               G[AGT]GC[ACT]^C
Bpu1102I      759  GCTGAGC              GC^T[ACGT]AGC
BsaI          782  GGTCTC               GGTCTC(1/5)
BsgI          941  GTGCAG               GTGCAG(16/14)
Bsp1286I      762  GAGCCC               G[AGT]GC[ACT]^C
BsrBI         824  CCGCTC               CCGCTC(-3/-3)
BstXI         348  CCAAACTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         567  AGATCC               [GA]^GATC[CT]
Bsu36I         17  CCTTAGG              CC^T[ACGT]AGG
CelII         759  GCTGAGC              GC^T[ACGT]AGC
DraII         233  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI          608  CCACGG               C^C[GA][CT]GG
Eam1105I      775  GACTGTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I         17  CCTTAGG              CC^T[ACGT]AGG
EcoNI          30  CCTTGACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      233  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI        1026  GAATTC               G^AATTC
EcoT14I       650  CCTTGG               C^C[AT][AT]GG
FauI          967  GCGGG                CCCGC(4/6)
HaeII          13  AGCGCC               [GA]GCGC^[CT]
MflI          567  AGATCC               [GA]^GATC[CT]
MspA1I        116  CAGCGG               C[AC]G^C[GT]G
NlaIV         233  GGGTCC               GG[ACGT]^[ACGT]CC
NspI          371  ACATGC               [GA]CATG^[CT]
PleI          124  GAGTC                GAGTC(4/5)
PpuMI         233  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI          238  CTGCAG               CTGCA^G
SapI          418  GAAGAGC              GCTCTTC(1/4)
ScaI           81  AGTACT               AGT^ACT
SexAI         174  ACCTGGT              A^CC[AT]GGT
SfaNI         297  GATGC                GCATC(5/9)
SfcI          238  CTGCAG               C^T[GA][CT]AG
StyI          650  CCTTGG               C^C[AT][AT]GG
TaqI          954  TCGA                 T^CGA
Tsp45I          5  GTGAC                ^GT[GC]AC
TthHB8I       954  TCGA                 T^CGA
XcmI          741  CCATGCGACCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         567  AGATCC               [GA]^GATC[CT]
XmnI          878  GAAACAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AvaII         234  GGTCC                G^G[AT]CC
             1077  GGACC               
BfaI         1039  CTAG                 C^TAG
             1066  CTAG                
BseRI         813  CTCCTC               GAGGAG(10/8)
              845  CTCCTC              
BsmAI         783  GTCTC                GTCTC(1/5)
              843  GTCTC               
BsmFI         112  GGGAC                GGGAC(10/14)
              657  GTCCC               
Cfr13I        234  GGTCC                G^G[ACGT]CC
             1077  GGACC               
MaeI         1039  CTAG                 C^TAG
             1066  CTAG                
MseI          707  TTAA                 T^TAA
              719  TTAA                
Sau96I        234  GGTCC                G^G[ACGT]CC
             1077  GGACC               
SmlI          337  CTCAAG               C^T[CT][GA]AG
              540  CTTGAG              
TfiI           98  GAATC                G^A[AT]TC
              533  GATTC               
Tru9I         707  TTAA                 T^TAA
              719  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BpmI        BsaJI       BsiYI       BslI        BsrI        
BssKI       BstNI       Cac8I       CfoI        DdeI        
DpnII       EarI        EcoRII      Fnu4HI      HaeIII      
HhaI        HinP1I      HinfI       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MnlI        
MslI        MvaI        MwoI        NdeII       NlaIII      
RsaI        Sau3AI      ScrFI       TseI        Tsp509I     
TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com