Restriction map for pF1KB9851
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspHI       AspI        
AvaI        AviII       AvrII       BanI        BbeI        
BbrPI       BbsI        BcgI        BfrI        BglII       
BlnI        BpmI        BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstUI       
BstZ17I     ClaI        CpoI        DraII       DrdI        
DsaI        EagI        EclXI       Eco47III    Eco52I      
EcoO109I    EcoRV       EcoT22I     FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AfaI          801  GTAC                 GT^AC
AhdI          868  GACCCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          856  CCTGAGG              CC^T[ACGT]AGG
ApaI          724  GGGCCC               GGGCC^C
AspEI         868  GACCCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        1084  GGATCC               G^GATCC
BanII         724  GGGCCC               G[GA]GC[CT]^C
BbvCI         313  GCTGAGG              CCTCAGC(-5/-2)
BciVI         710  GTATCC               GTATCC(6/5)
BclI          917  TGATCA               T^GATCA
BfaI          688  CTAG                 C^TAG
BmyI          724  GGGCCC               G[AGT]GC[ACT]^C
BsaI          175  GAGACC               GGTCTC(1/5)
BsmAI         175  GAGAC                GTCTC(1/5)
Bsp1286I      724  GGGCCC               G[AGT]GC[ACT]^C
BsrBI         304  GAGCGG               CCGCTC(-3/-3)
BsrFI        1039  ACCGGC               [GA]^CCGG[CT]
BstEII       1035  GGTGACC              G^GT[ACGT]ACC
BstPI        1035  GGTGACC              G^GT[ACGT]ACC
BstXI         811  CCAGAAAATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        856  CCTGAGG              CC^T[ACGT]AGG
CfoI         1062  GCGC                 GCG^C
Cfr10I       1039  ACCGGC               [GA]^CCGG[CT]
DraI         1126  TTTAAA               TTT^AAA
DraIII        464  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      868  GACCCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        856  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      1035  GGTGACC              G^GT[ACGT]ACC
FbaI          917  TGATCA               T^GATCA
HhaI         1062  GCGC                 GCG^C
HinP1I       1062  GCGC                 G^CGC
MaeI          688  CTAG                 C^TAG
MseI         1127  TTAA                 T^TAA
NspI          197  GCATGT               [GA]CATG^[CT]
PflMI         186  CCACCTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          874  GAGTC                GAGTC(4/5)
RsaI          801  GTAC                 GT^AC
SmlI          274  CTCAAG               C^T[CT][GA]AG
StuI           12  AGGCCT               AGG^CCT
TaqI          767  TCGA                 T^CGA
Tru9I        1127  TTAA                 T^TAA
TthHB8I       767  TCGA                 T^CGA
Van91I        186  CCACCTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AvaII         741  GGACC                G^G[AT]CC
              867  GGACC               
BcnI           54  CCGGG                CC^[GC]GG
              714  CCGGG               
BglI          437  GCCTCATGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              992  GCCGCCAAGGC         
BlpI          432  GCTGAGC              GC^T[ACGT]AGC
             1057  GCTAAGC             
Bpu1102I      432  GCTGAGC              GC^T[ACGT]AGC
             1057  GCTAAGC             
BstYI         672  GGATCT               [GA]^GATC[CT]
             1084  GGATCC              
CelII         432  GCTGAGC              GC^T[ACGT]AGC
             1057  GCTAAGC             
EcoNI         495  CCTGGTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              651  CCTGAACCAGG         
EcoRI           5  GAATTC               G^AATTC
              616  GAATTC              
EcoT14I       996  CCAAGG               C^C[AT][AT]GG
             1079  CCAAGG              
FauI          309  GCGGG                CCCGC(4/6)
              732  GCGGG               
MflI          672  GGATCT               [GA]^GATC[CT]
             1084  GGATCC              
MslI          213  CATTCGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              409  CACAATGATG          
MspA1I        328  CAGCTG               C[AC]G^C[GT]G
              483  CAGCTG              
NciI           54  CCGGG                CC^[GC]GG
              714  CCGGG               
PvuII         328  CAGCTG               CAG^CTG
              483  CAGCTG              
SapI          910  GAAGAGC              GCTCTTC(1/4)
              843  GCTCTTC             
StyI          996  CCAAGG               C^C[AT][AT]GG
             1079  CCAAGG              
TfiI          633  GATTC                G^A[AT]TC
              959  GAATC               
XhoII         672  GGATCT               [GA]^GATC[CT]
             1084  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        AlwNI       
ApoI        BalI        BbvI        BsaJI       BseRI       
BsiYI       BslI        BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
EaeI        EarI        EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PstI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TseI        Tsp45I      Tsp509I     TspRI       XcmI        
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