Restriction map for pF1KB9850
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BanI        
BanII       BbeI        BbsI        BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaI        BsiEI       BsiHKAI     BsiWI       BsmBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BspMI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MspA1I      MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccII         462  CGCG                 CG^CG
AccIII       1118  TCCGGA               T^CCGGA
Aor51HI       710  AGCGCT               AGC^GCT
AvaI          881  CTCGAG               C^[CT]CG[GA]G
BfaI         1030  CTAG                 C^TAG
BsaWI        1118  TCCGGA               [AT]^CCGG[AT]
BseAI        1118  TCCGGA               T^CCGGA
BseRI         547  GAGGAG               GAGGAG(10/8)
BsgI          363  CTGCAC               GTGCAG(16/14)
BsmAI         960  GTCTC                GTCTC(1/5)
BsmI           60  GAATGC               GAATGC(1/-1)
BsoBI         881  CTCGAG               C^[CT]CG[GA]G
BspEI        1118  TCCGGA               T^CCGGA
BsrBI         517  CCGCTC               CCGCTC(-3/-3)
BsrDI         393  CATTGC               GCAATG(2/0)
BstUI         462  CGCG                 CG^CG
DraI         1038  TTTAAA               TTT^AAA
Eco47III      710  AGCGCT               AGC^GCT
EcoT14I       536  CCAAGG               C^C[AT][AT]GG
FauI          516  CCCGC                CCCGC(4/6)
MaeI         1030  CTAG                 C^TAG
MaeIII        332  GTGAC                ^GT[ACGT]AC
MroI         1118  TCCGGA               T^CCGGA
MslI          382  CATCTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          462  CGCG                 CG^CG
PaeR7I        881  CTCGAG               C^TCGAG
PflMI         479  CCATTGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          985  GACTC                GAGTC(4/5)
SapI          519  GCTCTTC              GCTCTTC(1/4)
StyI          536  CCAAGG               C^C[AT][AT]GG
TaqI          882  TCGA                 T^CGA
TfiI          687  GAATC                G^A[AT]TC
Tsp45I        332  GTGAC                ^GT[GC]AC
Tsp509I      1107  AATT                 ^AATT
TthHB8I       882  TCGA                 T^CGA
Van91I        479  CCATTGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          881  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AfaI          175  GTAC                 GT^AC
              408  GTAC                
AlwNI         405  CAGGTACTG            CAG[ACGT][ACGT][ACGT]^CTG
              737  CAGGCTCTG           
BamHI          87  GGATCC               G^GATCC
              995  GGATCC              
BbrPI         357  CACGTG               CAC^GTG
              826  CACGTG              
BglII         113  AGATCT               A^GATCT
              581  AGATCT              
BmyI          787  GGGCAC               G[AGT]GC[ACT]^C
              851  GTGCCC              
BpmI          100  CTCCAG               CTGGAG(16/14)
              771  CTCCAG              
BsaAI         357  CACGTG               [CT]AC^GT[GA]
              826  CACGTG              
Bsp1286I      787  GGGCAC               G[AGT]GC[ACT]^C
              851  GTGCCC              
DdeI           75  CTCAG                C^T[ACGT]AG
              578  CTCAG               
DsaI          125  CCGTGG               C^C[GA][CT]GG
              719  CCACGG              
EarI          520  CTCTTC               CTCTTC(1/4)
             1081  GAAGAG              
EcoNI          30  CCTTCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              777  CCTCCATGAGG         
HindIII       838  AAGCTT               A^AGCTT
             1094  AAGCTT              
HinfI         687  GAATC                G^A[ACGT]TC
              985  GACTC               
Ksp632I       520  CTCTTC               CTCTTC(1/4)
             1081  GAAGAG              
MaeII         358  ACGT                 A^CGT
              827  ACGT                
MseI         1039  TTAA                 T^TAA
             1076  TTAA                
NlaIII        471  CATG                 CATG^
              781  CATG                
PmaCI         357  CACGTG               CAC^GTG
              826  CACGTG              
PmlI          357  CACGTG               CAC^GTG
              826  CACGTG              
PpuMI          94  AGGACCC              [GA]G^G[AT]CC[CT]
              748  GGGTCCT             
RsaI          175  GTAC                 GT^AC
              408  GTAC                
SmlI          109  CTCAAG               C^T[CT][GA]AG
              881  CTCGAG              
Tru9I        1039  TTAA                 T^TAA
             1076  TTAA                
XcmI          245  CCACCGACCTCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              896  CCACTAAAATCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AluI        AlwI        Asp700I     AvaII       
BalI        BbvI        BcnI        BsaJI       BsiYI       
BslI        BsmFI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DpnII       DraII       EaeI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HpaII       HphI        ItaI        
MboI        MboII       MflI        MluNI       MnlI        
MscI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIV       Sau3AI      Sau96I      ScrFI       
SfaNI       StuI        TseI        TspRI       XhoII       
XmnI        
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