Restriction map for pF1KB9850
 (Linear) (Six-base) MAPSORT of: KIBB9850.seq  Check: 8155  from: 1  to: 1131



KIBB9850.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AfeI AGC'GCT

Cuts at:      0     712    1131 
   Size:        712     419
 
AlwNI CAG_nnn'CTG

Cuts at:      0     410     742    1131 
   Size:        410     332     389

  Fragments arranged by size: 

                 410     389     332
 
AvaI C'yCGr_G

Cuts at:      0     881    1131 
   Size:        881     250
 
BamHI G'GATC_C

Cuts at:      0      87     995    1131 
   Size:         87     908     136

  Fragments arranged by size: 

                 908     136      87
 
BglII A'GATC_T

Cuts at:      0     113     581    1131 
   Size:        113     468     550

  Fragments arranged by size: 

                 550     468     113
 
Bme1580I G_kGCm'C

Cuts at:      0     791     855    1131 
   Size:        791      64     276

  Fragments arranged by size: 

                 791     276      64
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     420    1131 
   Size:        420     711
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     807    1131 
   Size:        807     324
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     756    1131 
   Size:         85     671     375

  Fragments arranged by size: 

                 671     375      85
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94    1131 
   Size:         94    1037
 
BsaAI yAC'GTr

Cuts at:      0     359     828    1131 
   Size:        359     469     303

  Fragments arranged by size: 

                 469     359     303
 
BsaWI w'CCGG_w

Cuts at:      0    1118    1131 
   Size:       1118      13
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     538     568    1131 
   Size:        538      30     563

  Fragments arranged by size: 

                 563     538      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     562    1131 
   Size:        562     569
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     348    1131 
   Size:        348     783
 
BsmI GAATG_Cn'

Cuts at:      0      66    1131 
   Size:         66    1065
 
Bsp1286I G_dGCh'C

Cuts at:      0     791     855    1131 
   Size:        791      64     276

  Fragments arranged by size: 

                 791     276      64
 
BspEI T'CCGG_A

Cuts at:      0    1118    1131 
   Size:       1118      13
 
BsrBI CCG'CTC

Cuts at:      0     519    1131 
   Size:        519     612
 
BsrDI GCAATG_nn'

Cuts at:      0     392    1131 
   Size:        392     739
 
BstYI r'GATC_y

Cuts at:      0      52      87     113     581     656     995    1131 
   Size:         52      35      26     468      75     339     136

  Fragments arranged by size: 

                 468     339     136      75      52      35      26
 
BtgI C'CryG_G

Cuts at:      0     125     719    1131 
   Size:        125     594     412

  Fragments arranged by size: 

                 594     412     125
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     270    1131 
   Size:        270     861
 
DraI TTT'AAA

Cuts at:      0    1040    1131 
   Size:       1040      91
 
EaeI y'GGCC_r

Cuts at:      0     149     239     318     456     464     651    1131 
   Size:        149      90      79     138       8     187     480

  Fragments arranged by size: 

                 480     187     149     138      90      79       8
 
EarI CTCTTCn'nnn_

Cuts at:      0     526    1076    1131 
   Size:        526     550      55

  Fragments arranged by size: 

                 550     526      55
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     756    1131 
   Size:         85     671     375

  Fragments arranged by size: 

                 671     375      85
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      34     781    1131 
   Size:         34     747     350

  Fragments arranged by size: 

                 747     350      34
 
EcoO109I rG'GnC_Cy

Cuts at:      0      95     611     749     923    1131 
   Size:         95     516     138     174     208

  Fragments arranged by size: 

                 516     208     174     138      95
 
HaeII r_GCGC'y

Cuts at:      0     109     714    1009    1131 
   Size:        109     605     295     122

  Fragments arranged by size: 

                 605     295     122     109
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     538     570    1131 
   Size:        538      32     561

  Fragments arranged by size: 

                 561     538      32
 
HindIII A'AGCT_T

Cuts at:      0     838    1094    1131 
   Size:        838     256      37

  Fragments arranged by size: 

                 838     256      37
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     536    1131 
   Size:         71     465     595

  Fragments arranged by size: 

                 595     465      71
 
MscI TGG'CCA

Cuts at:      0     151     241     320     458    1131 
   Size:        151      90      79     138     673

  Fragments arranged by size: 

                 673     151     138      90      79
 
MslI CAynn'nnrTG

Cuts at:      0     386    1131 
   Size:        386     745
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     485    1131 
   Size:        485     646
 
PmlI CAC'GTG

Cuts at:      0     359     828    1131 
   Size:        359     469     303

  Fragments arranged by size: 

                 469     359     303
 
PpuMI rG'GwC_Cy

Cuts at:      0      95     749    1131 
   Size:         95     654     382

  Fragments arranged by size: 

                 654     382      95
 
SapI GCTCTTCn'nnn_

Cuts at:      0     526    1131 
   Size:        526     605
 
SmlI C'TyrA_G

Cuts at:      0     109     881    1131 
   Size:        109     772     250

  Fragments arranged by size: 

                 772     250     109
 
StuI AGG'CCT

Cuts at:      0      29      43    1126    1131 
   Size:         29      14    1083       5

  Fragments arranged by size: 

                1083      29      14       5
 
StyI C'CwwG_G

Cuts at:      0     536    1131 
   Size:        536     595
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     316    1131 
   Size:        316     815
 
TatI w'GTAC_w

Cuts at:      0     174    1131 
   Size:        174     957
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     252     903    1131 
   Size:        252     651     228

  Fragments arranged by size: 

                 651     252     228
 
XhoI C'TCGA_G

Cuts at:      0     881    1131 
   Size:        881     250
 
XmnI GAAnn'nnTTC

Cuts at:      0      29     712    1085    1131 
   Size:         29     683     373      46

  Fragments arranged by size: 

                 683     373      46      29
 

 Enzymes that do cut:

     AfeI    AlwNI     AvaI    BamHI    BglII Bme1580I     BmrI     BplI
     BpmI    BpuEI    BsaAI    BsaWI    BsaXI    BseRI     BsgI     BsmI
 Bsp1286I    BspEI    BsrBI    BsrDI    BstYI     BtgI    BtgZI     DraI
     EaeI     EarI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I  HindIII
     MmeI     MscI     MslI    PflMI     PmlI    PpuMI     SapI     SmlI
     StuI     StyI    TaqII     TatI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmtI   Bpu10I     BsaI    BsaBI    BsaHI    BseYI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspHI    BspMI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI
     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TstI  Tth111I     XbaI     XmaI     ZraI
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