Restriction map for pF1KB9849
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AhdI        AlwNI       
AocI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       BamHI       
BbeI        BbrPI       BcgI        BciVI       BclI        
BfrI        BglII       BlpI        BpmI        Bpu1102I    
BsaBI       BsaI        BseAI       BseRI       BsgI        
BsmAI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssSI       Bst1107I    BstBI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        Eam1105I    Eco47III    Eco81I      EcoNI       
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        MamI        MfeI        MluI        MroI        
MseI        MunI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SbfI        ScaI        SexAI       
SfoI        SfuI        SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tru9I       Tsp509I     
TthHB8I     XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AcyI          400  GGCGTC               G[GA]^CG[CT]C
AgeI          389  ACCGGT               A^CCGGT
Alw44I        385  GTGCAC               G^TGCAC
AlwI         1019  GATCC                GGATC(4/5)
ApaLI         385  GTGCAC               G^TGCAC
AspI         1083  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         709  CCTAGG               C^CTAGG
BalI          881  TGGCCA               TGG^CCA
BbsI          757  GTCTTC               GAAGAC(2/6)
BbvCI         825  CCTCAGC              CCTCAGC(-5/-2)
BlnI          709  CCTAGG               C^CTAGG
BpuAI         757  GTCTTC               GAAGAC(2/6)
BsaHI         400  GGCGTC               G[GA]^CG[CT]C
BsaWI         389  ACCGGT               [AT]^CCGG[AT]
BsiEI         874  CGGCCG               CG[GA][CT]^CG
BsiWI         939  CGTACG               C^GTACG
BsmFI         691  GGGAC                GGGAC(10/14)
BsmI          195  GAATGC               GAATGC(1/-1)
BstAPI        238  GCACTGTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        867  GGTTACC              G^GT[ACGT]ACC
BstPI         867  GGTTACC              G^GT[ACGT]ACC
BstYI        1018  AGATCC               [GA]^GATC[CT]
DpnII        1019  GATC                 ^GATC
EagI          874  CGGCCG               C^GGCCG
EclXI         874  CGGCCG               C^GGCCG
Eco52I        874  CGGCCG               C^GGCCG
EcoO65I       867  GGTTACC              G^GT[ACGT]ACC
EcoT14I       709  CCTAGG               C^C[AT][AT]GG
HaeII         159  GGCGCT               [GA]GCGC^[CT]
Hin1I         400  GGCGTC               G[GA]^CG[CT]C
KspI          282  CCGCGG               CCGC^GG
MboI         1019  GATC                 ^GATC
MflI         1018  AGATCC               [GA]^GATC[CT]
MluNI         881  TGGCCA               TGG^CCA
MscI          881  TGGCCA               TGG^CCA
NaeI         1095  GCCGGC               GCC^GGC
NdeII        1019  GATC                 ^GATC
NgoMIV       1095  GCCGGC               G^CCGGC
NspI         1034  ACATGC               [GA]CATG^[CT]
PflFI        1083  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         964  CCACCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         389  ACCGGT               A^CCGGT
PpuMI         691  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII         828  CAGCTG               CAG^CTG
SacI          568  GAGCTC               GAGCT^C
SacII         282  CCGCGG               CCGC^GG
Sau3AI       1019  GATC                 ^GATC
SfiI          486  GGCCTGCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI         388  CACCGGTG             C[GA]^CCGG[CT]G
SmlI          184  CTCAAG               C^T[CT][GA]AG
StyI          709  CCTAGG               C^C[AT][AT]GG
Tth111I      1083  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        964  CCACCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        1018  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AfaI           96  GTAC                 GT^AC
              940  GTAC                
ApaI           15  GGGCCC               GGGCC^C
              437  GGGCCC              
AvaII         692  GGACC                G^G[AT]CC
              822  GGACC               
AviII         731  TGCGCA               TGC^GCA
             1013  TGCGCA              
BfaI          710  CTAG                 C^TAG
             1076  CTAG                
BglI          487  GCCTGCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              985  GCCACCCCGGC         
BsaAI          97  TACGTG               [CT]AC^GT[GA]
              652  TACGTG              
BspMI         373  ACCTGC               ACCTGC(4/8)
              544  ACCTGC              
BsrFI         389  ACCGGT               [GA]^CCGG[CT]
             1095  GCCGGC              
BssHII        524  GCGCGC               G^CGCGC
              526  GCGCGC              
BstXI         737  CCATCGCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              848  CCATCAACATGG        
Cfr10I        389  ACCGGT               [GA]^CCGG[CT]
             1095  GCCGGC              
FspI          731  TGCGCA               TGC^GCA
             1013  TGCGCA              
HgaI          199  GCGTC                GACGC(5/10)
              401  GCGTC               
HinfI        1072  GACTC                G^A[ACGT]TC
             1087  GAGTC               
MaeI          710  CTAG                 C^TAG
             1076  CTAG                
PleI         1072  GACTC                GAGTC(4/5)
             1087  GAGTC               
PstI          354  CTGCAG               CTGCA^G
              999  CTGCAG              
RsaI           96  GTAC                 GT^AC
              940  GTAC                
SapI          570  GCTCTTC              GCTCTTC(1/4)
              615  GCTCTTC             
Tsp45I        514  GTCAC                ^GT[GC]AC
              787  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AccII       AciI        AluI        AspHI       AvaI        
BanI        BanII       BbvI        BcnI        BmyI        
BsaJI       BsiHKAI     BsiYI       BslI        BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NlaIII      NlaIV       
Sau96I      ScrFI       SfaNI       SfcI        TseI        
TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com