Restriction map for pF1KB9849
 (Linear) (Six-base) MAPSORT of: KIBB9849.seq  Check: 6341  from: 1  to: 1131



KIBB9849.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     382    1131 
   Size:        382     749
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     285     558    1131 
   Size:        285     273     573

  Fragments arranged by size: 

                 573     285     273
 
AgeI A'CCGG_T

Cuts at:      0     389    1131 
   Size:        389     742
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     340     400     745    1131 
   Size:        340      60     345     386

  Fragments arranged by size: 

                 386     345     340      60
 
ApaI G_GGCC'C

Cuts at:      0      19     441    1131 
   Size:         19     422     690

  Fragments arranged by size: 

                 690     422      19
 
ApaLI G'TGCA_C

Cuts at:      0     385    1131 
   Size:        385     746
 
AvaI C'yCGr_G

Cuts at:      0     565     696     950    1131 
   Size:        565     131     254     181

  Fragments arranged by size: 

                 565     254     181     131
 
AvrII C'CTAG_G

Cuts at:      0     709    1131 
   Size:        709     422
 
BanI G'GyrC_C

Cuts at:      0      40      71     561     624    1131 
   Size:         40      31     490      63     507

  Fragments arranged by size: 

                 507     490      63      40      31
 
BanII G_rGCy'C

Cuts at:      0      19     441     572    1131 
   Size:         19     422     131     559

  Fragments arranged by size: 

                 559     422     131      19
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     750    1131 
   Size:        750     381
 
BbvCI CC'TCA_GC

Cuts at:      0     826    1131 
   Size:        826     305
 
BglI GCCn_nnn'nGGC

Cuts at:      0     493     991    1131 
   Size:        493     498     140

  Fragments arranged by size: 

                 498     493     140
 
Bme1580I G_kGCm'C

Cuts at:      0      19     389     441     629    1131 
   Size:         19     370      52     188     502

  Fragments arranged by size: 

                 502     370     188      52      19
 
BmgBI CAC'GTC

Cuts at:      0     786    1131 
   Size:        786     345
 
Bpu10I CC'TnA_GC

Cuts at:      0     826    1131 
   Size:        826     305
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169    1131 
   Size:        169     962
 
BsaAI yAC'GTr

Cuts at:      0      99     654    1131 
   Size:         99     555     477

  Fragments arranged by size: 

                 555     477      99
 
BsaHI Gr'CG_yC

Cuts at:      0     401    1131 
   Size:        401     730
 
BsaWI w'CCGG_w

Cuts at:      0     389    1131 
   Size:        389     742
 
BseYI C'CCAG_C

Cuts at:      0      60     428     606     925     976    1005    1131 
   Size:         60     368     178     319      51      29     126

  Fragments arranged by size: 

                 368     319     178     126      60      51      29
 
BsiEI CG_ry'CG

Cuts at:      0     877    1131 
   Size:        877     254
 
BsiHKAI G_wGCw'C

Cuts at:      0     314     389     506     572     659     914    1131 
   Size:        314      75     117      66      87     255     217

  Fragments arranged by size: 

                 314     255     217     117      87      75      66
 
BsiWI C'GTAC_G

Cuts at:      0     939    1131 
   Size:        939     192
 
BsmI GAATG_Cn'

Cuts at:      0     201    1131 
   Size:        201     930
 
Bsp1286I G_dGCh'C

Cuts at:      0      19     314     389     441     506     572     629     659 
   Size:         19     295      75      52      65      66      57      30

Cuts at:    659     914    1131 
   Size:        255     217

  Fragments arranged by size: 

                 295     255     217      75      66      65      57      52
                  30      19
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     382     553    1131 
   Size:        382     171     578

  Fragments arranged by size: 

                 578     382     171
 
BsrFI r'CCGG_y

Cuts at:      0     389    1095    1131 
   Size:        389     706      36

  Fragments arranged by size: 

                 706     389      36
 
BssHII G'CGCG_C

Cuts at:      0     524     526    1131 
   Size:        524       2     605

  Fragments arranged by size: 

                 605     524       2
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     244    1131 
   Size:        244     887
 
BstEII G'GTnAC_C

Cuts at:      0     867    1131 
   Size:        867     264
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     744     855    1131 
   Size:        744     111     276

  Fragments arranged by size: 

                 744     276     111
 
BstYI r'GATC_y

Cuts at:      0    1018    1131 
   Size:       1018     113
 
BtgI C'CryG_G

Cuts at:      0     155     282     743    1131 
   Size:        155     127     461     388

  Fragments arranged by size: 

                 461     388     155     127
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     723    1131 
   Size:        723     408
 
BtsI GCAGTG_nn'

Cuts at:      0    1071    1131 
   Size:       1071      60
 
EaeI y'GGCC_r

Cuts at:      0     458     874     881    1131 
   Size:        458     416       7     250

  Fragments arranged by size: 

                 458     416     250       7
 
EagI C'GGCC_G

Cuts at:      0     874    1131 
   Size:        874     257
 
EarI CTCTTCn'nnn_

Cuts at:      0     340     370     577     622    1131 
   Size:        340      30     207      45     509

  Fragments arranged by size: 

                 509     340     207      45      30
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1095    1131 
   Size:       1095      36
 
EcoICRI GAG'CTC

Cuts at:      0     570    1131 
   Size:        570     561
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     285     558    1131 
   Size:        285     273     573

  Fragments arranged by size: 

                 573     285     273
 
EcoO109I rG'GnC_Cy

Cuts at:      0      16     495     692    1131 
   Size:         16     479     197     439

  Fragments arranged by size: 

                 479     439     197      16
 
FspI TGC'GCA

Cuts at:      0     733    1015    1131 
   Size:        733     282     116

  Fragments arranged by size: 

                 733     282     116
 
FspAI rTGC'GCAy

Cuts at:      0     733    1131 
   Size:        733     398
 
HaeII r_GCGC'y

Cuts at:      0     163    1131 
   Size:        163     968
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1046    1131 
   Size:       1046      85
 
MscI TGG'CCA

Cuts at:      0     883    1131 
   Size:        883     248
 
MslI CAynn'nnrTG

Cuts at:      0     211     382     742     853    1131 
   Size:        211     171     360     111     278

  Fragments arranged by size: 

                 360     278     211     171     111
 
MspA1I CmG'CkG

Cuts at:      0     261     284     830     879    1131 
   Size:        261      23     546      49     252

  Fragments arranged by size: 

                 546     261     252      49      23
 
NaeI GCC'GGC

Cuts at:      0    1097    1131 
   Size:       1097      34
 
NgoMIV G'CCGG_C

Cuts at:      0    1095    1131 
   Size:       1095      36
 
NspI r_CATG'y

Cuts at:      0    1038    1131 
   Size:       1038      93
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     970    1131 
   Size:        970     161
 
PpuMI rG'GwC_Cy

Cuts at:      0     692    1131 
   Size:        692     439
 
PspOMI G'GGCC_C

Cuts at:      0      15     437    1131 
   Size:         15     422     694

  Fragments arranged by size: 

                 694     422      15
 
PstI C_TGCA'G

Cuts at:      0     358    1003    1131 
   Size:        358     645     128

  Fragments arranged by size: 

                 645     358     128
 
PvuII CAG'CTG

Cuts at:      0     830    1131 
   Size:        830     301
 
SacI G_AGCT'C

Cuts at:      0     572    1131 
   Size:        572     559
 
SacII CC_GC'GG

Cuts at:      0     285    1131 
   Size:        285     846
 
SapI GCTCTTCn'nnn_

Cuts at:      0     577     622    1131 
   Size:        577      45     509

  Fragments arranged by size: 

                 577     509      45
 
SfcI C'TryA_G

Cuts at:      0      66     354     591     999    1131 
   Size:         66     288     237     408     132

  Fragments arranged by size: 

                 408     288     237     132      66
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     493    1131 
   Size:        493     638
 
SgrAI Cr'CCGG_yG

Cuts at:      0     389    1131 
   Size:        389     742
 
SmlI C'TyrA_G

Cuts at:      0     184    1131 
   Size:        184     947
 
StyI C'CwwG_G

Cuts at:      0     709    1131 
   Size:        709     422
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     462     981    1131 
   Size:        462     519     150

  Fragments arranged by size: 

                 519     462     150
 
Tth111I GACn'n_nGTC

Cuts at:      0    1086    1131 
   Size:       1086      45
 

 Enzymes that do cut:

     AarI     AcuI     AgeI     AlfI     ApaI    ApaLI     AvaI    AvrII
     BanI    BanII     BbsI    BbvCI     BglI Bme1580I    BmgBI   Bpu10I
    BpuEI    BsaAI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI Bsp1286I    BspMI    BsrFI   BssHII   BstAPI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I     FspI    FspAI    HaeII     MmeI     MscI
     MslI   MspA1I     NaeI   NgoMIV     NspI    PflMI    PpuMI   PspOMI
     PstI    PvuII     SacI    SacII     SapI     SfcI     SfiI    SgrAI
     SmlI     StyI    TaqII  Tth111I

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AleI     AloI    AlwNI     ApoI     AscI     AseI    AsiSI     BaeI
    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
     BmrI     BmtI     BplI     BpmI     BsaI    BsaBI    BsaXI    BseRI
     BsgI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI    BssSI
    BstBI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI
    EcoNI    EcoRI    EcoRV     FalI     FseI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     NarI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SalI    SanDI     SbfI     ScaI    SexAI     SfoI     SgfI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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