Restriction map for pF1KB9848
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AfaI        AflII       AgeI        Alw44I      AlwI        
AlwNI       ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspHI       AspI        
BamHI       BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlpI        
Bpu1102I    BsaAI       BsaBI       BsaWI       BseAI       
BsiHKAI     BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BsrBI       BsrDI       
BsrGI       Bst1107I    BstAPI      BstBI       BstYI       
BstZ17I     CelII       ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        DsaI        EagI        
EclXI       Eco52I      EcoNI       EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MamI        
MboI        MfeI        MflI        MluI        MroI        
MseI        MunI        NcoI        NdeI        NdeII       
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        Sau3AI      SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII         801  GACGTC               GACGT^C
AflIII        296  ACATGT               A^C[GA][CT]GT
AhdI          675  GACCGTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          112  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       878  AGCGCT               AGC^GCT
AspEI         675  GACCGTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII         546  CCTAGG               C^CTAGG
BbeI         1012  GGCGCC               GGCGC^C
BbsI          964  GTCTTC               GAAGAC(2/6)
BfaI          547  CTAG                 C^TAG
BlnI          546  CCTAGG               C^CTAGG
BpuAI         964  GTCTTC               GAAGAC(2/6)
BsaI          470  GAGACC               GGTCTC(1/5)
BsgI          947  GTGCAG               GTGCAG(16/14)
BspLU11I      296  ACATGT               A^CATGT
BssHII        410  GCGCGC               G^CGCGC
BstEII        225  GGTAACC              G^GT[ACGT]ACC
BstPI         225  GGTAACC              G^GT[ACGT]ACC
Bsu36I        112  CCTGAGG              CC^T[ACGT]AGG
Eam1105I      675  GACCGTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          865  CTCTTC               CTCTTC(1/4)
Eco47III      878  AGCGCT               AGC^GCT
Eco81I        112  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       225  GGTAACC              G^GT[ACGT]ACC
KasI         1012  GGCGCC               G^GCGCC
Ksp632I       865  CTCTTC               CTCTTC(1/4)
MaeI          547  CTAG                 C^TAG
NaeI         1057  GCCGGC               GCC^GGC
NarI         1012  GGCGCC               GG^CGCC
NgoMIV       1057  GCCGGC               G^CCGGC
SapI          864  GCTCTTC              GCTCTTC(1/4)
SfaNI         540  GCATC                GCATC(5/9)
SfoI         1012  GGCGCC               GGC^GCC
SmlI          596  CTCAAG               C^T[CT][GA]AG
SphI          504  GCATGC               GCATG^C
StuI          569  AGGCCT               AGG^CCT
TaqI           71  TCGA                 T^CGA
Tsp45I        300  GTCAC                ^GT[GC]AC
TspRI        1067  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I        71  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI          801  GACGTC               G[GA]^CG[CT]C
             1012  GGCGCC              
AviII         507  TGCGCA               TGC^GCA
              971  TGCGCA              
BalI          426  TGGCCA               TGG^CCA
              986  TGGCCA              
BsaHI         801  GACGTC               G[GA]^CG[CT]C
             1012  GGCGCC              
BseRI         216  CTCCTC               GAGGAG(10/8)
             1021  GAGGAG              
BsiEI         649  CGGTCG               CG[GA][CT]^CG
              840  CGACCG              
BsmAI         470  GAGAC                GTCTC(1/5)
              594  GTCTC               
BsrFI         925  ACCGGC               [GA]^CCGG[CT]
             1057  GCCGGC              
BsrI          714  ACTGG                ACTGG(1/-1)
              860  ACTGG               
BstXI         380  CCAGCTGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              692  CCAACTGTCTGG        
Cfr10I        925  ACCGGC               [GA]^CCGG[CT]
             1057  GCCGGC              
EaeI          426  TGGCCA               [CT]^GGCC[GA]
              986  TGGCCA              
FspI          507  TGCGCA               TGC^GCA
              971  TGCGCA              
HgaI          630  GCGTC                GACGC(5/10)
              962  GCGTC               
Hin1I         801  GACGTC               G[GA]^CG[CT]C
             1012  GGCGCC              
HphI          333  TCACC                GGTGA(8/7)
              421  TCACC               
MaeII         464  ACGT                 A^CGT
              802  ACGT                
MaeIII        226  GTAAC                ^GT[ACGT]AC
              300  GTCAC               
MluNI         426  TGGCCA               TGG^CCA
              986  TGGCCA              
MscI          426  TGGCCA               TGG^CCA
              986  TGGCCA              
MslI          304  CACCATTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              499  CACCCGCATG          
NspI          296  ACATGT               [GA]CATG^[CT]
              504  GCATGC              
PleI          330  GACTC                GAGTC(4/5)
              592  GAGTC               
PpuMI         236  AGGACCT              [GA]G^G[AT]CC[CT]
              362  GGGACCT             
PvuII         381  CAGCTG               CAG^CTG
              524  CAGCTG              
TfiI          313  GAATC                G^A[AT]TC
              607  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AccII       AciI        AluI        ApaI        AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BspMI       BssKI       
BssSI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       ItaI        MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NlaIII      NlaIV       PstI        Sau96I      
ScrFI       SfcI        SmaI        StyI        TseI        
XmaCI       XmaI        
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