Restriction map for pF1KB9848
 (Linear) (Six-base) MAPSORT of: KIBB9848.seq  Check: 2092  from: 1  to: 1128



KIBB9848.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     830    1128 
   Size:        830     298
 
AatII G_ACGT'C

Cuts at:      0     805    1128 
   Size:        805     323
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     832    1128 
   Size:        427     405     296

  Fragments arranged by size: 

                 427     405     296
 
AfeI AGC'GCT

Cuts at:      0     880    1128 
   Size:        880     248
 
AflIII A'CryG_T

Cuts at:      0     296    1128 
   Size:        296     832
 
AhdI GACnn_n'nnGTC

Cuts at:      0     680    1128 
   Size:        680     448
 
ApaI G_GGCC'C

Cuts at:      0     175     271     734    1128 
   Size:        175      96     463     394

  Fragments arranged by size: 

                 463     394     175      96
 
AvaI C'yCGr_G

Cuts at:      0     264     432     774    1041    1078    1128 
   Size:        264     168     342     267      37      50

  Fragments arranged by size: 

                 342     267     264     168      50      37
 
AvrII C'CTAG_G

Cuts at:      0     546    1128 
   Size:        546     582
 
BanI G'GyrC_C

Cuts at:      0     376     654     928    1012    1128 
   Size:        376     278     274      84     116

  Fragments arranged by size: 

                 376     278     274     116      84
 
BanII G_rGCy'C

Cuts at:      0     112     175     271     723     734    1076    1128 
   Size:        112      63      96     452      11     342      52

  Fragments arranged by size: 

                 452     342     112      96      63      52      11
 
BbeI G_GCGC'C

Cuts at:      0    1016    1128 
   Size:       1016     112
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     957    1128 
   Size:        957     171
 
Bme1580I G_kGCm'C

Cuts at:      0     175     271     379     489     659     734    1128 
   Size:        175      96     108     110     170      75     394

  Fragments arranged by size: 

                 394     175     170     110     108      96      75
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     724    1128 
   Size:        724     404
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     369    1128 
   Size:        369     759
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     721     898    1128 
   Size:        167     554     177     230

  Fragments arranged by size: 

                 554     230     177     167
 
Bpu10I CC'TnA_GC

Cuts at:      0     566     666    1128 
   Size:        566     100     462

  Fragments arranged by size: 

                 566     462     100
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     581    1128 
   Size:        581     547
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     464    1128 
   Size:        464     664
 
BsaHI Gr'CG_yC

Cuts at:      0     802    1013    1128 
   Size:        802     211     115

  Fragments arranged by size: 

                 802     211     115
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     115     145    1128 
   Size:        115      30     983

  Fragments arranged by size: 

                 983     115      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     207    1036    1128 
   Size:        207     829      92

  Fragments arranged by size: 

                 829     207      92
 
BseYI C'CCAG_C

Cuts at:      0     168    1030    1128 
   Size:        168     862      98

  Fragments arranged by size: 

                 862     168      98
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     968    1128 
   Size:        968     160
 
BsiEI CG_ry'CG

Cuts at:      0     652     843    1128 
   Size:        652     191     285

  Fragments arranged by size: 

                 652     285     191
 
Bsp1286I G_dGCh'C

Cuts at:      0     112     175     271     379     489     659     723     734 
   Size:        112      63      96     108     110     170      64      11

Cuts at:    734    1076    1128 
   Size:        342      52

  Fragments arranged by size: 

                 342     170     112     110     108      96      64      63
                  52      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      83     238     830    1128 
   Size:         83     155     592     298

  Fragments arranged by size: 

                 592     298     155      83
 
BsrFI r'CCGG_y

Cuts at:      0     925    1057    1128 
   Size:        925     132      71

  Fragments arranged by size: 

                 925     132      71
 
BssHII G'CGCG_C

Cuts at:      0     410    1128 
   Size:        410     718
 
BssSI C'ACGA_G

Cuts at:      0     105     354    1006    1128 
   Size:        105     249     652     122

  Fragments arranged by size: 

                 652     249     122     105
 
BstEII G'GTnAC_C

Cuts at:      0     225    1128 
   Size:        225     903
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     387     699    1128 
   Size:        387     312     429

  Fragments arranged by size: 

                 429     387     312
 
Bsu36I CC'TnA_GG

Cuts at:      0     113    1128 
   Size:        113    1015
 
BtsI GCAGTG_nn'

Cuts at:      0    1073    1128 
   Size:       1073      55
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     463     498    1128 
   Size:        463      35     630

  Fragments arranged by size: 

                 630     463      35
 
EaeI y'GGCC_r

Cuts at:      0     426     986    1128 
   Size:        426     560     142

  Fragments arranged by size: 

                 560     426     142
 
EarI CTCTTCn'nnn_

Cuts at:      0     871    1128 
   Size:        871     257
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     721    1128 
   Size:        721     407
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     427     721     832     898    1128 
   Size:        167     260     294     111      66     230

  Fragments arranged by size: 

                 294     260     230     167     111      66
 
EcoO109I rG'GnC_Cy

Cuts at:      0     212     237     268     363    1128 
   Size:        212      25      31      95     765

  Fragments arranged by size: 

                 765     212      95      31      25
 
FspI TGC'GCA

Cuts at:      0     509     973    1128 
   Size:        509     464     155

  Fragments arranged by size: 

                 509     464     155
 
FspAI rTGC'GCAy

Cuts at:      0     509    1128 
   Size:        509     619
 
HaeII r_GCGC'y

Cuts at:      0      49     442     882     909    1016    1128 
   Size:         49     393     440      27     107     112

  Fragments arranged by size: 

                 440     393     112     107      49      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     115     147     666     698    1128 
   Size:        115      32     519      32     430

  Fragments arranged by size: 

                 519     430     115      32      32
 
KasI G'GCGC_C

Cuts at:      0    1012    1128 
   Size:       1012     116
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     447    1128 
   Size:        447     681
 
MscI TGG'CCA

Cuts at:      0     428     988    1128 
   Size:        428     560     140

  Fragments arranged by size: 

                 560     428     140
 
MslI CAynn'nnrTG

Cuts at:      0     308     503    1128 
   Size:        308     195     625

  Fragments arranged by size: 

                 625     308     195
 
MspA1I CmG'CkG

Cuts at:      0      10     138     168     383     526     836    1128 
   Size:         10     128      30     215     143     310     292

  Fragments arranged by size: 

                 310     292     215     143     128      30      10
 
NaeI GCC'GGC

Cuts at:      0    1059    1128 
   Size:       1059      69
 
NarI GG'CG_CC

Cuts at:      0    1013    1128 
   Size:       1013     115
 
NgoMIV G'CCGG_C

Cuts at:      0    1057    1128 
   Size:       1057      71
 
NspI r_CATG'y

Cuts at:      0     300     508    1128 
   Size:        300     208     620

  Fragments arranged by size: 

                 620     300     208
 
PciI A'CATG_T

Cuts at:      0     296    1128 
   Size:        296     832
 
PpuMI rG'GwC_Cy

Cuts at:      0     237     363    1128 
   Size:        237     126     765

  Fragments arranged by size: 

                 765     237     126
 
PspOMI G'GGCC_C

Cuts at:      0     171     267     730    1128 
   Size:        171      96     463     398

  Fragments arranged by size: 

                 463     398     171      96
 
PstI C_TGCA'G

Cuts at:      0      80     143     158     290     905     958    1128 
   Size:         80      63      15     132     615      53     170

  Fragments arranged by size: 

                 615     170     132      80      63      53      15
 
PvuII CAG'CTG

Cuts at:      0     383     526    1128 
   Size:        383     143     602

  Fragments arranged by size: 

                 602     383     143
 
SapI GCTCTTCn'nnn_

Cuts at:      0     871    1128 
   Size:        871     257
 
SfcI C'TryA_G

Cuts at:      0      76     139     154     286     901     954    1128 
   Size:         76      63      15     132     615      53     174

  Fragments arranged by size: 

                 615     174     132      76      63      53      15
 
SfoI GGC'GCC

Cuts at:      0    1014    1128 
   Size:       1014     114
 
SmaI CCC'GGG

Cuts at:      0     266     434     776    1080    1128 
   Size:        266     168     342     304      48

  Fragments arranged by size: 

                 342     304     266     168      48
 
SmlI C'TyrA_G

Cuts at:      0     596    1128 
   Size:        596     532
 
SphI G_CATG'C

Cuts at:      0     508    1128 
   Size:        508     620
 
StuI AGG'CCT

Cuts at:      0     571    1128 
   Size:        571     557
 
StyI C'CwwG_G

Cuts at:      0     257     546     572    1016    1128 
   Size:        257     289      26     444     112

  Fragments arranged by size: 

                 444     289     257     112      26
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     350    1128 
   Size:        350     778
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     340     372    1128 
   Size:        340      32     756

  Fragments arranged by size: 

                 756     340      32
 
XmaI C'CCGG_G

Cuts at:      0     264     432     774    1078    1128 
   Size:        264     168     342     304      50

  Fragments arranged by size: 

                 342     304     264     168      50
 
ZraI GAC'GTC

Cuts at:      0     803    1128 
   Size:        803     325
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI   AflIII     AhdI     ApaI     AvaI
    AvrII     BanI    BanII     BbeI     BbsI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI Bsp1286I    BspMI    BsrFI   BssHII    BssSI   BstEII
    BstXI   Bsu36I     BtsI    CspCI     EaeI     EarI     EciI  Eco57MI
 EcoO109I     FspI    FspAI    HaeII    Hin4I     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI    PpuMI
   PspOMI     PstI    PvuII     SapI     SfcI     SfoI     SmaI     SmlI
     SphI     StuI     StyI    TaqII     TstI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII     AgeI     AleI     AlfI     AloI
    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI    BamHI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI    BsaAI    BsaBI    BsaWI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BstAPI    BstBI
    BstYI  BstZ17I     BtgI    BtgZI     ClaI     DraI   DraIII     DrdI
     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI     TatI
  Tth111I     XbaI     XcmI     XhoI     XmnI
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