Restriction map for pF1KB9846
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

Acc65I      AccI        AccIII      AflII       AflIII      
AgeI        AhdI        AocI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbvCI       BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoO65I     EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MunI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshBI       PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        TaqI        Tru9I       Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII         268  GACGTC               GACGT^C
AclI          848  AACGTT               AA^CGTT
AcsI          729  GAATTC               [GA]^AATT[CT]
AfaI         1021  GTAC                 GT^AC
Alw44I        427  GTGCAC               G^TGCAC
AlwI           24  GGATC                GGATC(4/5)
ApaI          302  GGGCCC               GGGCC^C
ApaLI         427  GTGCAC               G^TGCAC
ApoI          729  GAATTC               [GA]^AATT[CT]
AspHI         427  GTGCAC               G[AT]GC[AT]^C
BanII         302  GGGCCC               G[GA]GC[CT]^C
BbsI          489  GAAGAC               GAAGAC(2/6)
BcgI           18  GCACCTGGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI         489  GAAGAC               GAAGAC(2/6)
BsiHKAI       427  GTGCAC               G[AT]GC[AT]^C
BspMI         119  ACCTGC               ACCTGC(4/8)
BsrBI         175  GAGCGG               CCGCTC(-3/-3)
BsrFI         413  GCCGGC               [GA]^CCGG[CT]
BssHII        112  GCGCGC               G^CGCGC
BstAPI        429  GCACATGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        413  GCCGGC               [GA]^CCGG[CT]
DpnII          25  GATC                 ^GATC
DraIII        328  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         939  CCTCTGTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         729  GAATTC               G^AATTC
HincII        845  GTCAAC               GT[CT]^[GA]AC
HindII        845  GTCAAC               GT[CT]^[GA]AC
MboI           25  GATC                 ^GATC
NaeI          413  GCCGGC               GCC^GGC
NdeII          25  GATC                 ^GATC
NgoMIV        413  GCCGGC               G^CCGGC
NspI          431  ACATGC               [GA]CATG^[CT]
PpuMI          77  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI         838  GACTGGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      848  AACGTT               AA^CGTT
RsaI         1021  GTAC                 GT^AC
SapI         1057  GCTCTTC              GCTCTTC(1/4)
Sau3AI         25  GATC                 ^GATC
SgrAI         412  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI          354  CCCGGG               CCC^GGG
SmlI           51  CTCAAG               C^T[CT][GA]AG
StuI         1107  AGGCCT               AGG^CCT
TfiI            4  GAATC                G^A[AT]TC
Tsp509I       730  AATT                 ^AATT
XmaCI         354  CCCGGG               C^CCGGG
XmaI          354  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccII         113  CGCG                 CG^CG
              757  CGCG                
AlwNI          16  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
               69  CAGCACCTG           
Aor51HI       581  AGCGCT               AGC^GCT
              804  AGCGCT              
Asp700I         4  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              594  GAACAACTTC          
AvaI          307  CTCGGG               C^[CT]CG[GA]G
              354  CCCGGG              
AvaII          78  GGACC                G^G[AT]CC
              223  GGTCC               
BfaI          709  CTAG                 C^TAG
              791  CTAG                
BseRI         376  GAGGAG               GAGGAG(10/8)
              913  GAGGAG              
BsgI          184  CTGCAC               GTGCAG(16/14)
              334  GTGCAG              
BsoBI         307  CTCGGG               C^[CT]CG[GA]G
              354  CCCGGG              
BstUI         113  CGCG                 CG^CG
              757  CGCG                
EarI         1058  CTCTTC               CTCTTC(1/4)
              658  GAAGAG              
Eco47III      581  AGCGCT               AGC^GCT
              804  AGCGCT              
HphI          609  GGTGA                GGTGA(8/7)
              685  TCACC               
Ksp632I      1058  CTCTTC               CTCTTC(1/4)
              658  GAAGAG              
MaeI          709  CTAG                 C^TAG
              791  CTAG                
MvnI          113  CGCG                 CG^CG
              757  CGCG                
NlaIII        151  CATG                 CATG^
              432  CATG                
PleI          478  GACTC                GAGTC(4/5)
              892  GAGTC               
PstI           13  CTGCAG               CTGCA^G
              520  CTGCAG              
SexAI         329  ACCTGGT              A^CC[AT]GGT
             1023  ACCTGGT             
XcmI          132  CCATCGGCGCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              678  CCACAAATCACCTGG     
XmnI            4  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              594  GAACAACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AcyI        AluI        BbvI        BcnI        
BmyI        BpmI        BsaHI       BsaI        BsaJI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       ItaI        MaeII       
MaeIII      MboII       MnlI        MspI        MvaI        
MwoI        NciI        NlaIV       Sau96I      ScrFI       
SfaNI       SfcI        TseI        Tsp45I      TspRI       
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