Restriction map for pF1KB9840
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaLI       ApoI        AscI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BbrPI       BbsI        
BbvCI       BcgI        BciVI       BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaI        BsiEI       BsiHKAI     BsiWI       
BsmI        BsoBI       Bsp1407I    BspDI       BspLU11I    
BsrDI       BsrGI       BssSI       Bst1107I    BstBI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoRI       FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
Ksp632I     MaeII       MfeI        MluI        MslI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SfcI        SfiI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII        162  TCCGGA               T^CCGGA
AocI          297  CCTGAGG              CC^T[ACGT]AGG
ApaI          929  GGGCCC               GGGCC^C
AseI          904  ATTAAT               AT^TAAT
AsnI          904  ATTAAT               AT^TAAT
BalI          704  TGGCCA               TGG^CCA
BamHI         113  GGATCC               G^GATCC
BanII         929  GGGCCC               G[GA]GC[CT]^C
BclI          221  TGATCA               T^GATCA
BfaI          655  CTAG                 C^TAG
BmyI          929  GGGCCC               G[AGT]GC[ACT]^C
BsaBI         138  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         162  TCCGGA               T^CCGGA
BsmBI         502  GAGACG               CGTCTC(1/5)
Bsp1286I      929  GGGCCC               G[AGT]GC[ACT]^C
BspEI         162  TCCGGA               T^CCGGA
BspHI         533  TCATGA               T^CATGA
BssHII        131  GCGCGC               G^CGCGC
BstAPI       1055  GCATGGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        291  GGTCACC              G^GT[ACGT]ACC
BstPI         291  GGTCACC              G^GT[ACGT]ACC
BstXI         947  CCAACCCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        297  CCTGAGG              CC^T[ACGT]AGG
DsaI          272  CCGCGG               C^C[GA][CT]GG
Eco81I        297  CCTGAGG              CC^T[ACGT]AGG
EcoNI         965  CCTCTGGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       291  GGTCACC              G^GT[ACGT]ACC
EcoRV         244  GATATC               GAT^ATC
EcoT14I       891  CCAAGG               C^C[AT][AT]GG
EcoT22I       824  ATGCAT               ATGCA^T
FbaI          221  TGATCA               T^GATCA
KspI          272  CCGCGG               CCGC^GG
MaeI          655  CTAG                 C^TAG
MamI          138  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         704  TGGCCA               TGG^CCA
MroI          162  TCCGGA               T^CCGGA
MscI          704  TGGCCA               TGG^CCA
MspA1I        272  CCGCGG               C[AC]G^C[GT]G
NsiI          824  ATGCAT               ATGCA^T
PflMI         633  CCATGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         728  GACAACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         904  ATTAAT               AT^TAAT
RcaI          533  TCATGA               T^CATGA
SacII         272  CCGCGG               CCGC^GG
SexAI         716  ACCAGGT              A^CC[AT]GGT
StyI          891  CCAAGG               C^C[AT][AT]GG
Van91I        633  CCATGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          654  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI          887  GGCGCC               G[GA]^CG[CT]C
              895  GGCGCC              
BbeI          887  GGCGCC               GGCGC^C
              895  GGCGCC              
BcnI          288  CCCGG                CC^[GC]GG
              926  CCGGG               
BsaHI         887  GGCGCC               G[GA]^CG[CT]C
              895  GGCGCC              
BsaWI         162  TCCGGA               [AT]^CCGG[AT]
              763  ACCGGA              
BseRI         963  CTCCTC               GAGGAG(10/8)
              121  GAGGAG              
BsgI          600  GTGCAG               GTGCAG(16/14)
             1062  GTGCAG              
BsmFI         645  GGGAC                GGGAC(10/14)
              760  GGGAC               
BspMI         387  GCAGGT               ACCTGC(4/8)
              975  GCAGGT              
BsrBI         694  CCGCTC               CCGCTC(-3/-3)
              427  GAGCGG              
BstF5I          3  GGATG                GGATG(2/0)
              822  GGATG               
DdeI          298  CTGAG                C^T[ACGT]AG
             1046  CTGAG               
DraII         283  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              928  GGGGCCC             
DraIII        318  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              465  CACCGAGTG           
EcoO109I      283  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              928  GGGGCCC             
FokI            3  GGATG                GGATG(9/13)
              822  GGATG               
HaeII         887  GGCGCC               [GA]GCGC^[CT]
              895  GGCGCC              
HgaI          504  GACGC                GACGC(5/10)
              607  GACGC               
Hin1I         887  GGCGCC               G[GA]^CG[CT]C
              895  GGCGCC              
KasI          887  GGCGCC               G^GCGCC
              895  GGCGCC              
MboII          90  GAAGA                GAAGA(8/7)
              241  GAAGA               
NaeI          312  GCCGGC               GCC^GGC
              558  GCCGGC              
NarI          887  GGCGCC               GG^CGCC
              895  GGCGCC              
NciI          288  CCCGG                CC^[GC]GG
              926  CCGGG               
NgoMIV        312  GCCGGC               G^CCGGC
              558  GCCGGC              
SfoI          887  GGCGCC               GGC^GCC
              895  GGCGCC              
XcmI          629  CCACCCATGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1001  CCAGTATTAGTCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaII       BanI        BbvI        BpmI        BsaJI       
BsiYI       BslI        BsmAI       BsrFI       BsrI        
BssKI       BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DpnII       EaeI        
EcoRII      FauI        Fnu4HI      HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MflI        MnlI        
MseI        MspI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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