Restriction map for pF1KB9835
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BciVI       BfrI        BglI        BglII       BlnI        
BpmI        BpuAI       BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco47III    Eco81I      
EcoO65I     EcoRI       EcoRV       FauI        FseI        
FspI        Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       StuI        SwaI        TaqI        Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccI          226  GTCTAC               GT^[AC][GT]AC
AfaI          680  GTAC                 GT^AC
AlwNI          81  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          854  GGGCCC               GGGCC^C
BalI          410  TGGCCA               TGG^CCA
BamHI         608  GGATCC               G^GATCC
BanII         854  GGGCCC               G[GA]GC[CT]^C
BcgI          839  GCAACAACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          125  TGATCA               T^GATCA
BcnI          852  CCGGG                CC^[GC]GG
BlpI          153  GCTGAGC              GC^T[ACGT]AGC
BmyI          854  GGGCCC               G[AGT]GC[ACT]^C
Bpu1102I      153  GCTGAGC              GC^T[ACGT]AGC
BsaI          336  GGTCTC               GGTCTC(1/5)
Bsp1286I      854  GGGCCC               G[AGT]GC[ACT]^C
BspHI         800  TCATGA               T^CATGA
BsrBI         210  CCGCTC               CCGCTC(-3/-3)
BstXI          19  CCACAGATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         153  GCTGAGC              GC^T[ACGT]AGC
CfoI          834  GCGC                 GCG^C
DsaI          939  CCATGG               C^C[GA][CT]GG
EarI         1038  CTCTTC               CTCTTC(1/4)
EcoNI         324  CCTGTGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       964  ATGCAT               ATGCA^T
FbaI          125  TGATCA               T^GATCA
HaeII         833  AGCGCC               [GA]GCGC^[CT]
HapII         852  CCGG                 C^CGG
HgaI          447  GCGTC                GACGC(5/10)
HhaI          834  GCGC                 GCG^C
HinP1I        834  GCGC                 G^CGC
HincII        355  GTCAAC               GT[CT]^[GA]AC
HindII        355  GTCAAC               GT[CT]^[GA]AC
HpaII         852  CCGG                 C^CGG
Ksp632I      1038  CTCTTC               CTCTTC(1/4)
MaeII         996  ACGT                 A^CGT
MluNI         410  TGGCCA               TGG^CCA
MscI          410  TGGCCA               TGG^CCA
MseI          689  TTAA                 T^TAA
MspA1I        640  CCGCTG               C[AC]G^C[GT]G
MspI          852  CCGG                 C^CGG
NciI          852  CCGGG                CC^[GC]GG
NcoI          939  CCATGG               C^CATGG
NsiI          964  ATGCAT               ATGCA^T
NspI           56  GCATGC               [GA]CATG^[CT]
PstI           65  CTGCAG               CTGCA^G
RcaI          800  TCATGA               T^CATGA
RsaI          680  GTAC                 GT^AC
SexAI         776  ACCTGGT              A^CC[AT]GGT
SmlI          949  CTCAAG               C^T[CT][GA]AG
SphI           56  GCATGC               GCATG^C
SspI            7  AATATT               AAT^ATT
Tru9I         689  TTAA                 T^TAA
XcmI          866  CCACTGAGATTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AcsI           45  AAATTT               [GA]^AATT[CT]
              930  AAATTT              
AluI          826  AGCT                 AG^CT
              892  AGCT                
ApoI           45  AAATTT               [GA]^AATT[CT]
              930  AAATTT              
BfaI           94  CTAG                 C^TAG
              142  CTAG                
BsiEI         207  CGGCCG               CG[GA][CT]^CG
              849  CGGCCG              
BsmFI         483  GGGAC                GGGAC(10/14)
              501  GTCCC               
BspMI          63  ACCTGC               ACCTGC(4/8)
              230  ACCTGC              
EagI          207  CGGCCG               C^GGCCG
              849  CGGCCG              
EclXI         207  CGGCCG               C^GGCCG
              849  CGGCCG              
Eco52I        207  CGGCCG               C^GGCCG
              849  CGGCCG              
MaeI           94  CTAG                 C^TAG
              142  CTAG                
PpuMI         566  AGGTCCT              [GA]G^G[AT]CC[CT]
              805  AGGACCC             
SfcI           65  CTGCAG               C^T[GA][CT]AG
              363  CTACAG              
Tsp45I        217  GTCAC                ^GT[GC]AC
              449  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AciI        AlwI        AvaII       BbvI        BsaJI       
BsiYI       BslI        BsmAI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EaeI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        MaeIII      MboI        
MboII       MflI        MnlI        MslI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        TfiI        
TseI        Tsp509I     TspRI       XhoII       
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