Restriction map for pF1KB9827
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AlwI        AocI        Aor51HI     ApaI        ApoI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvrII       BamHI       BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BlnI        BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrGI       BssSI       Bst1107I    
BstBI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      DrdI        Eam1105I    Eco47III    
Eco81I      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MseI        MslI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfoI        SfuI        SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TfiI        Tru9I       Tsp509I     
TspRI       Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Alw44I        808  GTGCAC               G^TGCAC
ApaLI         808  GTGCAC               G^TGCAC
AscI          410  GGCGCGCC             GG^CGCGCC
AspHI         808  GTGCAC               G[AT]GC[AT]^C
AvaII         720  GGACC                G^G[AT]CC
BalI          263  TGGCCA               TGG^CCA
BanI           16  GGCACC               G^G[CT][GA]CC
BcnI          224  CCCGG                CC^[GC]GG
BfaI          370  CTAG                 C^TAG
BglII         347  AGATCT               A^GATCT
BlpI          828  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      828  GCTCAGC              GC^T[ACGT]AGC
BsaI          112  GAGACC               GGTCTC(1/5)
BsgI          625  GTGCAG               GTGCAG(16/14)
BsiHKAI       808  GTGCAC               G[AT]GC[AT]^C
BsmAI         112  GAGAC                GTCTC(1/5)
BspMI         129  GCAGGT               ACCTGC(4/8)
BsrI          572  ACTGG                ACTGG(1/-1)
BssHII        411  GCGCGC               G^CGCGC
BstXI         185  CCAGCACGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         347  AGATCT               [GA]^GATC[CT]
CelII         828  GCTCAGC              GC^T[ACGT]AGC
DdeI          829  CTCAG                C^T[ACGT]AG
DpnII         348  GATC                 ^GATC
EagI          398  CGGCCG               C^GGCCG
EarI          793  CTCTTC               CTCTTC(1/4)
EclXI         398  CGGCCG               C^GGCCG
Eco52I        398  CGGCCG               C^GGCCG
EcoNI         636  CCTGAAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       842  CCATGG               C^C[AT][AT]GG
HinfI         856  GAGTC                G^A[ACGT]TC
Ksp632I       793  CTCTTC               CTCTTC(1/4)
MaeI          370  CTAG                 C^TAG
MboI          348  GATC                 ^GATC
MflI          347  AGATCT               [GA]^GATC[CT]
MluNI         263  TGGCCA               TGG^CCA
MscI          263  TGGCCA               TGG^CCA
NaeI          688  GCCGGC               GCC^GGC
NciI          224  CCCGG                CC^[GC]GG
NcoI          842  CCATGG               C^CATGG
NdeII         348  GATC                 ^GATC
NgoMIV        688  GCCGGC               G^CCGGC
PleI          856  GAGTC                GAGTC(4/5)
PpuMI         719  AGGACCT              [GA]G^G[AT]CC[CT]
SapI          792  GCTCTTC              GCTCTTC(1/4)
Sau3AI        348  GATC                 ^GATC
SfaNI         101  GCATC                GCATC(5/9)
SfiI          390  GGCCGACGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI          932  AGGCCT               AGG^CCT
StyI          842  CCATGG               C^C[AT][AT]GG
XhoII         347  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcyI          160  GACGCC               G[GA]^CG[CT]C
              835  GACGCC              
AvaI          582  CTCGGG               C^[CT]CG[GA]G
              943  CCCGAG              
BglI          163  GCCGTGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              391  GCCGACGCGGC         
BmyI           15  GGGCAC               G[AGT]GC[ACT]^C
              808  GTGCAC              
BpmI          280  CTGGAG               CTGGAG(16/14)
              673  CTGGAG              
BsaHI         160  GACGCC               G[GA]^CG[CT]C
              835  GACGCC              
BsiEI         398  CGGCCG               CG[GA][CT]^CG
              468  CGACCG              
BsmFI         575  GGGAC                GGGAC(10/14)
              956  GGGAC               
BsoBI         582  CTCGGG               C^[CT]CG[GA]G
              943  CCCGAG              
Bsp1286I       15  GGGCAC               G[AGT]GC[ACT]^C
              808  GTGCAC              
BstEII        201  GGTGACC              G^GT[ACGT]ACC
              558  GGTCACC             
BstPI         201  GGTGACC              G^GT[ACGT]ACC
              558  GGTCACC             
DraII         233  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              719  AGGACCT             
DsaI          164  CCGTGG               C^C[GA][CT]GG
              842  CCATGG              
EaeI          263  TGGCCA               [CT]^GGCC[GA]
              398  CGGCCG              
EcoO109I      233  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              719  AGGACCT             
EcoO65I       201  GGTGACC              G^GT[ACGT]ACC
              558  GGTCACC             
HaeII         269  AGCGCC               [GA]GCGC^[CT]
              645  GGCGCT              
Hin1I         160  GACGCC               G[GA]^CG[CT]C
              835  GACGCC              
HphI          201  GGTGA                GGTGA(8/7)
              560  TCACC               
MaeII         440  ACGT                 A^CGT
              818  ACGT                
MaeIII        202  GTGAC                ^GT[ACGT]AC
              559  GTCAC               
NlaIV          16  GGCACC               GG[ACGT]^[ACGT]CC
              491  GGCTCC              
PflMI         273  CCAGCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              920  CCAGCCGCTGG         
PstI          295  CTGCAG               CTGCA^G
              649  CTGCAG              
PvuII         259  CAGCTG               CAG^CTG
              885  CAGCTG              
SfcI          295  CTGCAG               C^T[GA][CT]AG
              649  CTGCAG              
SgrAI         589  CACCGGCG             C[GA]^CCGG[CT]G
              687  CGCCGGCG            
SmlI           52  CTCAAG               C^T[CT][GA]AG
              121  CTCAAG              
TaqI          380  TCGA                 T^CGA
              803  TCGA                
Tsp45I        202  GTGAC                ^GT[GC]AC
              559  GTCAC               
TthHB8I       380  TCGA                 T^CGA
              803  TCGA                
Van91I        273  CCAGCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              920  CCAGCCGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AviII       BbvI        BsaJI       BseRI       BsiYI       
BslI        BsrBI       BsrFI       BssKI       BstAPI      
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      EcoRII      FauI        Fnu4HI      
FokI        FspI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HpaII       ItaI        MboII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NlaIII      RsaI        Sau96I      ScrFI       
TseI        
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