Restriction map for pF1KB9826
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AlwNI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvrII       
BamHI       BbrPI       BbsI        BbvCI       BciVI       
BfrI        BglII       BlnI        BpuAI       BsaI        
BsaWI       BseAI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    EarI        EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        HincII      
HindII      HindIII     HinfI       HpaI        KpnI        
Ksp632I     MaeIII      MfeI        MluI        MroI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RsrII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        TaqI        
TfiI        Tsp45I      Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AcyI          610  GGCGCC               G[GA]^CG[CT]C
Alw44I         64  GTGCAC               G^TGCAC
AocI          462  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       507  AGCGCT               AGC^GCT
ApaI          264  GGGCCC               GGGCC^C
ApaLI          64  GTGCAC               G^TGCAC
AvaII         336  GGACC                G^G[AT]CC
AviII         641  TGCGCA               TGC^GCA
BalI          836  TGGCCA               TGG^CCA
BbeI          610  GGCGCC               GGCGC^C
BcgI          200  CGAAGGCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          350  TGATCA               T^GATCA
BfaI          136  CTAG                 C^TAG
BglI          235  GCCCACCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          540  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      540  GCTGAGC              GC^T[ACGT]AGC
BsaHI         610  GGCGCC               G[GA]^CG[CT]C
BsiEI         146  CGGCCG               CG[GA][CT]^CG
BsmAI         952  GTCTC                GTCTC(1/5)
BspHI         575  TCATGA               T^CATGA
BsrBI         149  CCGCTC               CCGCTC(-3/-3)
BssSI         307  CACGAG               CACGAG(-5/-1)
BstAPI        288  GCAGCTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI          18  GGATCT               [GA]^GATC[CT]
Bsu36I        462  CCTGAGG              CC^T[ACGT]AGG
CelII         540  GCTGAGC              GC^T[ACGT]AGC
EagI          146  CGGCCG               C^GGCCG
EclXI         146  CGGCCG               C^GGCCG
Eco47III      507  AGCGCT               AGC^GCT
Eco52I        146  CGGCCG               C^GGCCG
Eco81I        462  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       485  CCATGG               C^C[AT][AT]GG
FauI          912  GCGGG                CCCGC(4/6)
FbaI          350  TGATCA               T^GATCA
FspI          641  TGCGCA               TGC^GCA
HgaI          746  GCGTC                GACGC(5/10)
Hin1I         610  GGCGCC               G[GA]^CG[CT]C
KasI          610  GGCGCC               G^GCGCC
KspI          606  CCGCGG               CCGC^GG
MaeI          136  CTAG                 C^TAG
MflI           18  GGATCT               [GA]^GATC[CT]
MluNI         836  TGGCCA               TGG^CCA
MscI          836  TGGCCA               TGG^CCA
MseI          848  TTAA                 T^TAA
NarI          610  GGCGCC               GG^CGCC
NcoI          485  CCATGG               C^CATGG
NspI          551  GCATGC               [GA]CATG^[CT]
PstI          208  CTGCAG               CTGCA^G
PvuII         289  CAGCTG               CAG^CTG
RcaI          575  TCATGA               T^CATGA
SacII         606  CCGCGG               CCGC^GG
SfcI          208  CTGCAG               C^T[GA][CT]AG
SfoI          610  GGCGCC               GGC^GCC
SphI          551  GCATGC               GCATG^C
StyI          485  CCATGG               C^C[AT][AT]GG
Tru9I         848  TTAA                 T^TAA
Tsp509I       727  AATT                 ^AATT
XhoII          18  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI          321  GTAC                 GT^AC
              738  GTAC                
AlwI          579  GATCC                GGATC(4/5)
               18  GGATC               
BanI          270  GGCACC               G^G[CT][GA]CC
              610  GGCGCC              
BpmI          511  CTGGAG               CTGGAG(16/14)
              892  CTGGAG              
BsaBI         348  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              816  GATGCAAATC          
BsmFI         686  GGGAC                GGGAC(10/14)
              748  GTCCC               
DsaI          485  CCATGG               C^C[GA][CT]GG
              606  CCGCGG              
EaeI          146  CGGCCG               [CT]^GGCC[GA]
              836  TGGCCA              
EcoNI         130  CCTCCTCTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              207  CCTGCAGCAGG         
HphI          372  GGTGA                GGTGA(8/7)
              809  TCACC               
MamI          348  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              816  GATGCAAATC          
MspA1I        289  CAGCTG               C[AC]G^C[GT]G
              606  CCGCGG              
RsaI          321  GTAC                 GT^AC
              738  GTAC                
SacI          310  GAGCTC               GAGCT^C
              905  GAGCTC              
SmaI          443  CCCGGG               CCC^GGG
              791  CCCGGG              
StuI          564  AGGCCT               AGG^CCT
              863  AGGCCT              
XmaCI         443  CCCGGG               C^CCGGG
              791  CCCGGG              
XmaI          443  CCCGGG               C^CCGGG
              791  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AccII       AciI        AluI        AspHI       AvaI        
BanII       BbvI        BcnI        BmyI        BsaAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsoBI       Bsp1286I    BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EcoO109I    EcoRII      
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HpaII       ItaI        MaeII       
MboI        MboII       MnlI        MslI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
SfaNI       TseI        TspRI       
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