Restriction map for pF1KB9826
 (Linear) (Six-base) MAPSORT of: KIBB9826.seq  Check: 9129  from: 1  to: 963



KIBB9826.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     754     963 
   Size:        754     209
 
AfeI AGC'GCT

Cuts at:      0     509     963 
   Size:        509     454
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     233     568     963 
   Size:        233     335     395

  Fragments arranged by size: 

                 395     335     233
 
ApaI G_GGCC'C

Cuts at:      0     268     963 
   Size:        268     695
 
ApaLI G'TGCA_C

Cuts at:      0      64     963 
   Size:         64     899
 
AvaI C'yCGr_G

Cuts at:      0     246     443     517     791     963 
   Size:        246     197      74     274     172

  Fragments arranged by size: 

                 274     246     197     172      74
 
BanI G'GyrC_C

Cuts at:      0     270     610     963 
   Size:        270     340     353

  Fragments arranged by size: 

                 353     340     270
 
BanII G_rGCy'C

Cuts at:      0     130     268     314     518     790     909     963 
   Size:        130     138      46     204     272     119      54

  Fragments arranged by size: 

                 272     204     138     130     119      54      46
 
BbeI G_GCGC'C

Cuts at:      0     614     963 
   Size:        614     349
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     189     223     963 
   Size:        189      34     740

  Fragments arranged by size: 

                 740     189      34
 
BclI T'GATC_A

Cuts at:      0     350     963 
   Size:        350     613
 
BglI GCCn_nnn'nGGC

Cuts at:      0     241     963 
   Size:        241     722
 
BlpI GC'TnA_GC

Cuts at:      0     541     963 
   Size:        541     422
 
Bme1580I G_kGCm'C

Cuts at:      0      68     268     963 
   Size:         68     200     695

  Fragments arranged by size: 

                 695     200      68
 
BmgBI CAC'GTC

Cuts at:      0     690     963 
   Size:        690     273
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     149     963 
   Size:        149     814
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     532     913     963 
   Size:        532     381      50

  Fragments arranged by size: 

                 532     381      50
 
Bpu10I CC'TnA_GC

Cuts at:      0     583     963 
   Size:        583     380
 
BsaAI yAC'GTr

Cuts at:      0     324     597     756     963 
   Size:        324     273     159     207

  Fragments arranged by size: 

                 324     273     207     159
 
BsaBI GATnn'nnATC

Cuts at:      0     352     820     963 
   Size:        352     468     143

  Fragments arranged by size: 

                 468     352     143
 
BsaHI Gr'CG_yC

Cuts at:      0     611     963 
   Size:        611     352
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     135     165     963 
   Size:        135      30     798

  Fragments arranged by size: 

                 798     135      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     122     238     535     724     910     963 
   Size:        122     116     297     189     186      53

  Fragments arranged by size: 

                 297     189     186     122     116      53
 
BseYI C'CCAG_C

Cuts at:      0     257     963 
   Size:        257     706
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     426     442     463     963 
   Size:        316     110      16      21     500

  Fragments arranged by size: 

                 500     316     110      21      16
 
BsiEI CG_ry'CG

Cuts at:      0     149     963 
   Size:        149     814
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     314     909     963 
   Size:         68     246     595      54

  Fragments arranged by size: 

                 595     246      68      54
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     130     268     314     518     790     909     963 
   Size:         68      62     138      46     204     272     119      54

  Fragments arranged by size: 

                 272     204     138     119      68      62      54      46
            
 
BspHI T'CATG_A

Cuts at:      0     575     963 
   Size:        575     388
 
BsrBI CCG'CTC

Cuts at:      0     151     963 
   Size:        151     812
 
BssSI C'ACGA_G

Cuts at:      0     307     963 
   Size:        307     656
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     294     963 
   Size:        294     669
 
BstYI r'GATC_y

Cuts at:      0      18     963 
   Size:         18     945
 
Bsu36I CC'TnA_GG

Cuts at:      0     463     963 
   Size:        463     500
 
BtgI C'CryG_G

Cuts at:      0     485     606     963 
   Size:        485     121     357

  Fragments arranged by size: 

                 485     357     121
 
BtsI GCAGTG_nn'

Cuts at:      0     926     954     963 
   Size:        926      28       9

  Fragments arranged by size: 

                 926      28       9
 
EaeI y'GGCC_r

Cuts at:      0     146     836     963 
   Size:        146     690     127

  Fragments arranged by size: 

                 690     146     127
 
EagI C'GGCC_G

Cuts at:      0     146     963 
   Size:        146     817
 
EcoICRI GAG'CTC

Cuts at:      0     312     907     963 
   Size:        312     595      56

  Fragments arranged by size: 

                 595     312      56
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     532     754     913     963 
   Size:        532     222     159      50

  Fragments arranged by size: 

                 532     222     159      50
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     134     211     963 
   Size:        134      77     752

  Fragments arranged by size: 

                 752     134      77
 
EcoO109I rG'GnC_Cy

Cuts at:      0      48     177     204     243     963 
   Size:         48     129      27      39     720

  Fragments arranged by size: 

                 720     129      48      39      27
 
FspI TGC'GCA

Cuts at:      0     643     963 
   Size:        643     320
 
HaeII r_GCGC'y

Cuts at:      0     511     541     614     626     963 
   Size:        511      30      73      12     337

  Fragments arranged by size: 

                 511     337      73      30      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     135     167     963 
   Size:        135      32     796

  Fragments arranged by size: 

                 796     135      32
 
KasI G'GCGC_C

Cuts at:      0     610     963 
   Size:        610     353
 
MscI TGG'CCA

Cuts at:      0     838     963 
   Size:        838     125
 
MslI CAynn'nnrTG

Cuts at:      0     556     595     814     963 
   Size:        556      39     219     149

  Fragments arranged by size: 

                 556     219     149      39
 
MspA1I CmG'CkG

Cuts at:      0     291     608     963 
   Size:        291     317     355

  Fragments arranged by size: 

                 355     317     291
 
NarI GG'CG_CC

Cuts at:      0     611     963 
   Size:        611     352
 
NcoI C'CATG_G

Cuts at:      0     485     963 
   Size:        485     478
 
NspI r_CATG'y

Cuts at:      0     555     963 
   Size:        555     408
 
PasI CC'CwG_GG

Cuts at:      0      76     963 
   Size:         76     887
 
PspOMI G'GGCC_C

Cuts at:      0     264     963 
   Size:        264     699
 
PstI C_TGCA'G

Cuts at:      0     212     963 
   Size:        212     751
 
PvuII CAG'CTG

Cuts at:      0     291     963 
   Size:        291     672
 
SacI G_AGCT'C

Cuts at:      0     314     909     963 
   Size:        314     595      54

  Fragments arranged by size: 

                 595     314      54
 
SacII CC_GC'GG

Cuts at:      0     609     963 
   Size:        609     354
 
SfcI C'TryA_G

Cuts at:      0     208     963 
   Size:        208     755
 
SfoI GGC'GCC

Cuts at:      0     612     963 
   Size:        612     351
 
SmaI CCC'GGG

Cuts at:      0     445     793     963 
   Size:        445     348     170

  Fragments arranged by size: 

                 445     348     170
 
SphI G_CATG'C

Cuts at:      0     555     963 
   Size:        555     408
 
StuI AGG'CCT

Cuts at:      0     566     865     963 
   Size:        566     299      98

  Fragments arranged by size: 

                 566     299      98
 
StyI C'CwwG_G

Cuts at:      0     485     963 
   Size:        485     478
 
XmaI C'CCGG_G

Cuts at:      0     443     791     963 
   Size:        443     348     172

  Fragments arranged by size: 

                 443     348     172
 

 Enzymes that do cut:

     AcuI     AfeI     AlfI     ApaI    ApaLI     AvaI     BanI    BanII
     BbeI     BcgI     BclI     BglI     BlpI Bme1580I    BmgBI     BplI
     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BsrBI    BssSI   BstAPI
    BstYI   Bsu36I     BtgI     BtsI     EaeI     EagI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FspI    HaeII    Hin4I     KasI     MscI     MslI
   MspA1I     NarI     NcoI     NspI     PasI   PspOMI     PstI    PvuII
     SacI    SacII     SfcI     SfoI     SmaI     SphI     StuI     StyI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI    BbvCI    BciVI    BfrBI    BglII
     BmrI     BmtI    BpuEI     BsaI    BsaWI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BstBI   BstEII
    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EarI     EciI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     NaeI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SrfI
     SspI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI
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