Restriction map for pF1KB9820
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflIII      AgeI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AvaI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbsI        BcgI        BclI        BcnI        BlnI        
BpuAI       BsaAI       BsaBI       BsaHI       BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      Cac8I       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HpaI        KasI        KpnI        KspI        MaeII       
MamI        MluI        MslI        MspA1I      MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TthHB8I     XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccIII        696  TCCGGA               T^CCGGA
AcsI           25  GAATTT               [GA]^AATT[CT]
AfaI          101  GTAC                 GT^AC
AflII         769  CTTAAG               C^TTAAG
AhdI          349  GACGAAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          306  GGATC                GGATC(4/5)
ApoI           25  GAATTT               [GA]^AATT[CT]
AspEI         349  GACGAAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          191  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI           45  TGGCCA               TGG^CCA
BanII         457  GAGCTC               G[GA]GC[CT]^C
BciVI         434  GTATCC               GTATCC(6/5)
BfaI          607  CTAG                 C^TAG
BfrI          769  CTTAAG               C^TTAAG
BglI          263  GCCCCCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         650  AGATCT               A^GATCT
BlpI          124  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      124  GCTGAGC              GC^T[ACGT]AGC
BsaI          189  GAGACC               GGTCTC(1/5)
BsaWI         696  TCCGGA               [AT]^CCGG[AT]
BseAI         696  TCCGGA               T^CCGGA
BsgI          643  GTGCAG               GTGCAG(16/14)
BspEI         696  TCCGGA               T^CCGGA
BssSI         851  CTCGTG               CACGAG(-5/-1)
CelII         124  GCTGAGC              GC^T[ACGT]AGC
DsaI           42  CCATGG               C^C[GA][CT]GG
EaeI           45  TGGCCA               [CT]^GGCC[GA]
Eam1105I      349  GACGAAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         566  CCTGGCAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII         697  CCGG                 C^CGG
HincII        912  GTTGAC               GT[CT]^[GA]AC
HindII        912  GTTGAC               GT[CT]^[GA]AC
HpaII         697  CCGG                 C^CGG
HphI          662  TCACC                GGTGA(8/7)
MaeI          607  CTAG                 C^TAG
MfeI          585  CAATTG               C^AATTG
MluNI          45  TGGCCA               TGG^CCA
MroI          696  TCCGGA               T^CCGGA
MscI           45  TGGCCA               TGG^CCA
MspI          697  CCGG                 C^CGG
MunI          585  CAATTG               C^AATTG
NcoI           42  CCATGG               C^CATGG
PflFI         191  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         227  CCAATCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI          50  AGGTCCC              [GA]G^G[AT]CC[CT]
RsaI          101  GTAC                 GT^AC
SacI          457  GAGCTC               GAGCT^C
SapI          708  GAAGAGC              GCTCTTC(1/4)
SmlI          769  CTTAAG               C^T[CT][GA]AG
TfiI          881  GAATC                G^A[AT]TC
Tsp45I        449  GTGAC                ^GT[GC]AC
Tth111I       191  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        227  CCAATCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AciI          831  CCGC                 CCGC(-3/-1)
              834  CCGC                
BanI          756  GGTGCC               G^G[CT][GA]CC
              827  GGCACC              
BbvCI         331  CCTCAGC              CCTCAGC(-5/-2)
              475  GCTGAGG             
BbvI          272  GCAGC                GCAGC(8/12)
               60  GCTGC               
BpmI          402  CTCCAG               CTGGAG(16/14)
              499  CTCCAG              
BstYI         306  GGATCT               [GA]^GATC[CT]
              650  AGATCT              
EcoT14I        42  CCATGG               C^C[AT][AT]GG
              267  CCTTGG              
MaeIII        449  GTGAC                ^GT[ACGT]AC
              521  GTTAC               
MflI          306  GGATCT               [GA]^GATC[CT]
              650  AGATCT              
MwoI          263  GCCCCCTTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              704  GCTGGAAGAGC         
StyI           42  CCATGG               C^C[AT][AT]GG
              267  CCTTGG              
TseI           60  GCTGC                G^C[AT]GC
              272  GCAGC               
XhoII         306  GGATCT               [GA]^GATC[CT]
              650  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AluI        AspHI       AvaII       BmyI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DpnII       DraII       
EarI        EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       ItaI        Ksp632I     MboI        
MboII       MnlI        MseI        MvaI        NdeII       
NlaIII      NlaIV       PleI        Sau3AI      Sau96I      
ScrFI       Tru9I       Tsp509I     TspRI       
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