Restriction map for pF1KB9820
 (Linear) (Six-base) MAPSORT of: KIBB9820.seq  Check: 6991  from: 1  to: 933



KIBB9820.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AflII C'TTAA_G

Cuts at:      0     769     933 
   Size:        769     164
 
AhdI GACnn_n'nnGTC

Cuts at:      0     354     933 
   Size:        354     579
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     152     933 
   Size:        152     781
 
ApoI r'AATT_y

Cuts at:      0      25     933 
   Size:         25     908
 
BanI G'GyrC_C

Cuts at:      0     756     827     933 
   Size:        756      71     106

  Fragments arranged by size: 

                 756     106      71
 
BanII G_rGCy'C

Cuts at:      0     461     933 
   Size:        461     472
 
BbvCI CC'TCA_GC

Cuts at:      0     332     476     933 
   Size:        332     144     457

  Fragments arranged by size: 

                 457     332     144
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     445     933 
   Size:        445     488
 
BglI GCCn_nnn'nGGC

Cuts at:      0     269     933 
   Size:        269     664
 
BglII A'GATC_T

Cuts at:      0     650     933 
   Size:        650     283
 
BlpI GC'TnA_GC

Cuts at:      0     125     933 
   Size:        125     808
 
Bme1580I G_kGCm'C

Cuts at:      0     265     830     933 
   Size:        265     565     103

  Fragments arranged by size: 

                 565     265     103
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      78     188     287     440     933 
   Size:         78     110      99     153     493

  Fragments arranged by size: 

                 493     153     110      99      78
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     387     484     933 
   Size:        387      97     449

  Fragments arranged by size: 

                 449     387      97
 
Bpu10I CC'TnA_GC

Cuts at:      0     332     476     858     933 
   Size:        332     144     382      75

  Fragments arranged by size: 

                 382     332     144      75
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     183     933 
   Size:        183     750
 
BsaWI w'CCGG_w

Cuts at:      0     696     933 
   Size:        696     237
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     180     210     478     508     933 
   Size:        180      30     268      30     425

  Fragments arranged by size: 

                 425     268     180      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     201     484     859     933 
   Size:        201     283     375      74

  Fragments arranged by size: 

                 375     283     201      74
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     664     933 
   Size:        664     269
 
BsiHKAI G_wGCw'C

Cuts at:      0     118     461     715     933 
   Size:        118     343     254     218

  Fragments arranged by size: 

                 343     254     218     118
 
Bsp1286I G_dGCh'C

Cuts at:      0     118     265     461     715     830     933 
   Size:        118     147     196     254     115     103

  Fragments arranged by size: 

                 254     196     147     118     115     103
 
BspEI T'CCGG_A

Cuts at:      0     696     933 
   Size:        696     237
 
BssSI C'ACGA_G

Cuts at:      0     851     933 
   Size:        851      82
 
BstYI r'GATC_y

Cuts at:      0     306     650     933 
   Size:        306     344     283

  Fragments arranged by size: 

                 344     306     283
 
BtgI C'CryG_G

Cuts at:      0      42     933 
   Size:         42     891
 
EaeI y'GGCC_r

Cuts at:      0      45     933 
   Size:         45     888
 
EarI CTCTTCn'nnn_

Cuts at:      0     178     461     703     933 
   Size:        178     283     242     230

  Fragments arranged by size: 

                 283     242     230     178
 
EcoICRI GAG'CTC

Cuts at:      0     459     933 
   Size:        459     474
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     387     484     933 
   Size:        387      97     449

  Fragments arranged by size: 

                 449     387      97
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     570     933 
   Size:        570     363
 
EcoO109I rG'GnC_Cy

Cuts at:      0      51     282     575     933 
   Size:         51     231     293     358

  Fragments arranged by size: 

                 358     293     231      51
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     100     132     180     212     340     372     478     510 
   Size:        100      32      48      32     128      32     106      32

Cuts at:    510     933 
   Size:        423

  Fragments arranged by size: 

                 423     128     106     100      48      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     914     933 
   Size:        914      19
 
MfeI C'AATT_G

Cuts at:      0     585     933 
   Size:        585     348
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     132     933 
   Size:        132     801
 
MscI TGG'CCA

Cuts at:      0      47     933 
   Size:         47     886
 
NcoI C'CATG_G

Cuts at:      0      42     933 
   Size:         42     891
 
PasI CC'CwG_GG

Cuts at:      0      55     933 
   Size:         55     878
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     233     933 
   Size:        233     700
 
PfoI T'CCnGG_A

Cuts at:      0     737     933 
   Size:        737     196
 
PpuMI rG'GwC_Cy

Cuts at:      0      51     933 
   Size:         51     882
 
SacI G_AGCT'C

Cuts at:      0     461     933 
   Size:        461     472
 
SapI GCTCTTCn'nnn_

Cuts at:      0     703     933 
   Size:        703     230
 
SmlI C'TyrA_G

Cuts at:      0     769     933 
   Size:        769     164
 
StyI C'CwwG_G

Cuts at:      0      42     267     933 
   Size:         42     225     666

  Fragments arranged by size: 

                 666     225      42
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     231     933 
   Size:        231     702
 
Tth111I GACn'n_nGTC

Cuts at:      0     194     933 
   Size:        194     739
 

 Enzymes that do cut:

    AflII     AhdI     AlfI     ApoI     BanI    BanII    BbvCI    BciVI
     BglI    BglII     BlpI Bme1580I     BmrI     BpmI   Bpu10I     BsaI
    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I    BspEI    BssSI
    BstYI     BtgI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    Hin4I   HincII     MfeI     MmeI     MscI     NcoI     PasI    PflMI
     PfoI    PpuMI     SacI     SapI     SmlI     StyI    TaqII  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI     BcgI
     BclI    BfrBI    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaBI
    BsaHI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MluI
     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     TstI     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com