Restriction map for pF1KB9817
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AgeI        
AlwI        AocI        Aor51HI     ApaI        AscI        
Asp700I     Asp718I     AspI        AvaI        AviII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlpI        
Bpu1102I    BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsoBI       BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DpnII       DraIII      DrdI        
DsaI        EaeI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MamI        MboI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MspI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AflIII        824  ACGTGT               A^C[GA][CT]GT
AhdI          498  GACAGAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        686  GTGCAC               G^TGCAC
AlwNI         871  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         686  GTGCAC               G^TGCAC
AseI          454  ATTAAT               AT^TAAT
AsnI          454  ATTAAT               AT^TAAT
AspEI         498  GACAGAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         686  GTGCAC               G[AT]GC[AT]^C
AvaII         101  GGTCC                G^G[AT]CC
AvrII         510  CCTAGG               C^CTAGG
BbsI          660  GTCTTC               GAAGAC(2/6)
BlnI          510  CCTAGG               C^CTAGG
BmyI          686  GTGCAC               G[AGT]GC[ACT]^C
BpmI           85  CTGGAG               CTGGAG(16/14)
BpuAI         660  GTCTTC               GAAGAC(2/6)
BsaAI         548  TACGTG               [CT]AC^GT[GA]
BsiHKAI       686  GTGCAC               G[AT]GC[AT]^C
BsmAI         506  GTCTC                GTCTC(1/5)
BsmBI         505  CGTCTC               CGTCTC(1/5)
Bsp1286I      686  GTGCAC               G[AGT]GC[ACT]^C
Bsp1407I      325  TGTACA               T^GTACA
BspMI         338  GCAGGT               ACCTGC(4/8)
BsrGI         325  TGTACA               T^GTACA
BstAPI        722  GCAAAGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         371  TTCGAA               TT^CGAA
CfoI          731  GCGC                 GCG^C
DraI          130  TTTAAA               TTT^AAA
DraII          80  AGGCCCT              [GA]G^G[ACGT]CC[CT]
Eam1105I      498  GACAGAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI           24  GAAGAG               CTCTTC(1/4)
EcoO109I       80  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRV         281  GATATC               GAT^ATC
EcoT14I       510  CCTAGG               C^C[AT][AT]GG
HhaI          731  GCGC                 GCG^C
HinP1I        731  GCGC                 G^CGC
Ksp632I        24  GAAGAG               CTCTTC(1/4)
MaeIII        698  GTGAC                ^GT[ACGT]AC
MfeI          329  CAATTG               C^AATTG
MunI          329  CAATTG               C^AATTG
NlaIV         101  GGTCCC               GG[ACGT]^[ACGT]CC
NspV          371  TTCGAA               TT^CGAA
PshBI         454  ATTAAT               AT^TAAT
PstI          868  CTGCAG               CTGCA^G
SfcI          868  CTGCAG               C^T[GA][CT]AG
SfuI          371  TTCGAA               TT^CGAA
SspBI         325  TGTACA               T^GTACA
SspI          244  AATATT               AAT^ATT
StyI          510  CCTAGG               C^C[AT][AT]GG
Tsp45I        698  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI          417  GAATTT               [GA]^AATT[CT]
              604  GAATTT              
ApoI          417  GAATTT               [GA]^AATT[CT]
              604  GAATTT              
BbvI           90  GCAGC                GCAGC(8/12)
              870  GCAGC               
BfaI           94  CTAG                 C^TAG
              511  CTAG                
BsmFI         228  GGGAC                GGGAC(10/14)
              102  GTCCC               
BsmI           18  GAATGC               GAATGC(1/-1)
              727  GAATGC              
BssKI          84  CCTGG                ^CC[ACGT]GG
              136  CCTGG               
BstF5I         60  GGATG                GGATG(2/0)
              772  GGATG               
BstNI          84  CCTGG                CC^[AT]GG
              136  CCTGG               
EcoRII         84  CCTGG                ^CC[AT]GG
              136  CCTGG               
Fnu4HI         90  GCAGC                GC^[ACGT]GC
              870  GCAGC               
FokI           60  GGATG                GGATG(9/13)
              772  GGATG               
HaeIII         81  GGCC                 GG^CC
              749  GGCC                
HinfI         197  GATTC                G^A[ACGT]TC
              887  GAATC               
HphI          165  GGTGA                GGTGA(8/7)
              200  TCACC               
ItaI           90  GCAGC                GC^[ACGT]GC
              870  GCAGC               
MaeI           94  CTAG                 C^TAG
              511  CTAG                
MvaI           84  CCTGG                CC^[AT]GG
              136  CCTGG               
ScrFI          84  CCTGG                CC^[ACGT]GG
              136  CCTGG               
TaqI          372  TCGA                 T^CGA
              890  TCGA                
TfiI          197  GATTC                G^A[AT]TC
              887  GAATC               
TseI           90  GCAGC                G^C[AT]GC
              870  GCAGC               
TthHB8I       372  TCGA                 T^CGA
              890  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AfaI        AluI        BsaJI       BsiYI       BslI        
BsrI        Cac8I       Cfr13I      DdeI        MaeII       
MboII       MnlI        MseI        MwoI        NlaIII      
RsaI        Sau96I      SfaNI       Tru9I       Tsp509I     
TspRI       
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