Restriction map for pF1KB9807
##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
BalI        BamHI       BanI        BbeI        BbrPI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlpI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BsrI        BssHII      
Bst1107I    BstAPI      BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MaeII       MaeIII      MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tsp45I      Tth111I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        


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# ONE CUT ENZYMES (   49 enzymes) #
###################################

AfaI          444  GTAC                 GT^AC
AlwI          259  GGATC                GGATC(4/5)
ApaI          644  GGGCCC               GGGCC^C
AvrII         610  CCTAGG               C^CTAGG
BanII         644  GGGCCC               G[GA]GC[CT]^C
BbvCI         388  GCTGAGG              CCTCAGC(-5/-2)
BlnI          610  CCTAGG               C^CTAGG
BmyI          644  GGGCCC               G[AGT]GC[ACT]^C
BsmFI         656  GGGAC                GGGAC(10/14)
BsmI           29  GAATGC               GAATGC(1/-1)
Bsp1286I      644  GGGCCC               G[AGT]GC[ACT]^C
BsrDI         488  GCAATG               GCAATG(2/0)
BssKI         404  CCAGG                ^CC[ACGT]GG
BssSI          76  CACGAG               CACGAG(-5/-1)
BstBI          36  TTCGAA               TT^CGAA
BstNI         404  CCAGG                CC^[AT]GG
Cac8I         435  GCTGGC               GC[ACGT]^[ACGT]GC
CfoI          381  GCGC                 GCG^C
DpnII         260  GATC                 ^GATC
EarI           10  CTCTTC               CTCTTC(1/4)
EcoRI         625  GAATTC               G^AATTC
EcoRII        404  CCAGG                ^CC[AT]GG
FauI          199  GCGGG                CCCGC(4/6)
HaeII         380  AGCGCC               [GA]GCGC^[CT]
HaeIII        645  GGCC                 GG^CC
HhaI          381  GCGC                 GCG^C
HinP1I        381  GCGC                 G^CGC
HinfI          39  GAATC                G^A[ACGT]TC
HphI          304  GGTGA                GGTGA(8/7)
Ksp632I        10  CTCTTC               CTCTTC(1/4)
MboI          260  GATC                 ^GATC
MseI          172  TTAA                 T^TAA
MspA1I        386  CAGCTG               C[AC]G^C[GT]G
MvaI          404  CCAGG                CC^[AT]GG
MwoI          199  GCGGGAAAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         260  GATC                 ^GATC
NspV           36  TTCGAA               TT^CGAA
PpuMI         596  AGGACCC              [GA]G^G[AT]CC[CT]
PvuII         386  CAGCTG               CAG^CTG
RsaI          444  GTAC                 GT^AC
Sau3AI        260  GATC                 ^GATC
ScrFI         404  CCAGG                CC^[ACGT]GG
SfuI           36  TTCGAA               TT^CGAA
SmlI          448  CTCAAG               C^T[CT][GA]AG
TaqI           37  TCGA                 T^CGA
TfiI           39  GAATC                G^A[AT]TC
Tru9I         172  TTAA                 T^TAA
TthHB8I        37  TCGA                 T^CGA
XcmI          425  CCAACAAGGGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AciI           73  CCGC                 CCGC(-3/-1)
              199  GCGG                
AluI           96  AGCT                 AG^CT
              387  AGCT                
Asp700I        29  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              664  GAAGACATTC          
AvaII         597  GGACC                G^G[AT]CC
              616  GGTCC               
BbsI          109  GAAGAC               GAAGAC(2/6)
              664  GAAGAC              
BbvI          370  GCTGC                GCAGC(8/12)
              638  GCTGC               
BfaI          524  CTAG                 C^TAG
              611  CTAG                
BpuAI         109  GAAGAC               GAAGAC(2/6)
              664  GAAGAC              
BstF5I        393  GGATG                GGATG(2/0)
              578  GGATG               
DdeI          389  CTGAG                C^T[ACGT]AG
              440  CTCAG               
EcoT14I       610  CCTAGG               C^C[AT][AT]GG
              619  CCAAGG              
Fnu4HI        370  GCTGC                GC^[ACGT]GC
              638  GCTGC               
FokI          393  GGATG                GGATG(9/13)
              578  GGATG               
ItaI          370  GCTGC                GC^[ACGT]GC
              638  GCTGC               
MaeI          524  CTAG                 C^TAG
              611  CTAG                
PstI          371  CTGCAG               CTGCA^G
              639  CTGCAG              
SfaNI         249  GATGC                GCATC(5/9)
              579  GATGC               
SfcI          371  CTGCAG               C^T[GA][CT]AG
              639  CTGCAG              
StyI          610  CCTAGG               C^C[AT][AT]GG
              619  CCAAGG              
TseI          370  GCTGC                G^C[AT]GC
              638  GCTGC               
TspRI         321  CAGTG                CA[GC]TG[ACGT][ACGT]^
              660  CACTG               
XmnI           29  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              664  GAAGACATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AcsI        ApoI        BsaJI       BsiYI       BslI        
Cfr13I      DraII       EcoO109I    MboII       MnlI        
NlaIII      NlaIV       Sau96I      Tsp509I     
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