Restriction map for pF1KB9803
##################################
# NO CUT ENZYMES (  181 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BanI        BbeI        BbrPI       BbvCI       BbvI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        Ksp632I     MaeI        MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccII         281  CGCG                 CG^CG
AfaI          505  GTAC                 GT^AC
AluI          455  AGCT                 AG^CT
AvaII         212  GGTCC                G^G[AT]CC
BamHI         510  GGATCC               G^GATCC
BanII         443  GGGCTC               G[GA]GC[CT]^C
BbsI          120  GAAGAC               GAAGAC(2/6)
BpuAI         120  GAAGAC               GAAGAC(2/6)
BsiYI         276  CCAGCCGCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          276  CCAGCCGCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          127  GAATGC               GAATGC(1/-1)
Bsp1407I      504  TGTACA               T^GTACA
BspMI         209  GCAGGT               ACCTGC(4/8)
BsrGI         504  TGTACA               T^GTACA
BsrI          177  ACTGG                ACTGG(1/-1)
BstUI         281  CGCG                 CG^CG
BstYI         510  GGATCC               [GA]^GATC[CT]
CfoI          297  GCGC                 GCG^C
DpnII         511  GATC                 ^GATC
DsaI          280  CCGCGG               C^C[GA][CT]GG
EcoRV         586  GATATC               GAT^ATC
FauI          282  GCGGG                CCCGC(4/6)
HaeII         296  AGCGCC               [GA]GCGC^[CT]
HhaI          297  GCGC                 GCG^C
HinP1I        297  GCGC                 G^CGC
KspI          280  CCGCGG               CCGC^GG
MaeII         110  ACGT                 A^CGT
MboI          511  GATC                 ^GATC
MflI          510  GGATCC               [GA]^GATC[CT]
MspA1I        280  CCGCGG               C[AC]G^C[GT]G
MvnI          281  CGCG                 CG^CG
NdeII         511  GATC                 ^GATC
PleI          370  GACTC                GAGTC(4/5)
RsaI          505  GTAC                 GT^AC
SacII         280  CCGCGG               CCGC^GG
Sau3AI        511  GATC                 ^GATC
SmlI          446  CTCAAG               C^T[CT][GA]AG
SspBI         504  TGTACA               T^GTACA
TfiI          358  GATTC                G^A[AT]TC
TspRI         405  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         510  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwI          511  GATCC                GGATC(4/5)
              510  GGATC               
BcnI          300  CCCGG                CC^[GC]GG
              320  CCCGG               
BglI          253  GCCTCCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              569  GCCAGAGGGGC         
BmyI          316  GTGCCC               G[AGT]GC[ACT]^C
              443  GGGCTC              
BseRI         409  GAGGAG               GAGGAG(10/8)
              451  GAGGAG              
Bsp1286I      316  GTGCCC               G[AGT]GC[ACT]^C
              443  GGGCTC              
BstF5I        147  GGATG                GGATG(2/0)
              491  GGATG               
EcoT14I       365  CCAAGG               C^C[AT][AT]GG
              523  CCAAGG              
Fnu4HI        279  GCCGC                GC^[ACGT]GC
              286  GCCGC               
FokI          147  GGATG                GGATG(9/13)
              491  GGATG               
HaeIII        285  GGCC                 GG^CC
              568  GGCC                
HapII         301  CCGG                 C^CGG
              321  CCGG                
HinfI         358  GATTC                G^A[ACGT]TC
              370  GACTC               
HpaII         301  CCGG                 C^CGG
              321  CCGG                
HphI          163  GGTGA                GGTGA(8/7)
              220  GGTGA               
ItaI          279  GCCGC                GC^[ACGT]GC
              286  GCCGC               
MspI          301  CCGG                 C^CGG
              321  CCGG                
MwoI          253  GCCTCCCAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              569  GCCAGAGGGGC         
NciI          300  CCCGG                CC^[GC]GG
              320  CCCGG               
NlaIV         510  GGATCC               GG[ACGT]^[ACGT]CC
              566  GGGGCC              
SfaNI         492  GATGC                GCATC(5/9)
              396  GCATC               
StyI          365  CCAAGG               C^C[AT][AT]GG
              523  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AciI        BsaJI       BsmFI       BssKI       BstNI       
Cac8I       Cfr13I      DdeI        EcoRII      MboII       
MnlI        MvaI        Sau96I      ScrFI       
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