Restriction map for pF1KB9802
##################################
# NO CUT ENZYMES (  175 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AhdI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BmyI        BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Cac8I       
CfoI        Cfr13I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        HphI        
KasI        KpnI        KspI        MaeIII      MamI        
MfeI        MluI        MroI        MunI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tsp45I      TspRI       Tth111I     
Van91I      XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AciI          261  GCGG                 CCGC(-3/-1)
AcsI           76  AAATTC               [GA]^AATT[CT]
AgeI          527  ACCGGT               A^CCGGT
AocI           25  CCTAAGG              CC^T[ACGT]AGG
ApoI           76  AAATTC               [GA]^AATT[CT]
Asp700I       128  GAAAGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI         264  GGATCC               G^GATCC
BlpI          325  GCTCAGC              GC^T[ACGT]AGC
BpmI          409  CTGGAG               CTGGAG(16/14)
Bpu1102I      325  GCTCAGC              GC^T[ACGT]AGC
BsaWI         527  ACCGGT               [AT]^CCGG[AT]
BsiYI         207  CCGATACCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          207  CCGATACCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          37  GTCTC                GTCTC(1/5)
BspMI         363  GCAGGT               ACCTGC(4/8)
BsrFI         527  ACCGGT               [GA]^CCGG[CT]
BsrI          350  ACTGG                ACTGG(1/-1)
BstF5I        293  CATCC                GGATG(2/0)
Bsu36I         25  CCTAAGG              CC^T[ACGT]AGG
CelII         325  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        527  ACCGGT               [GA]^CCGG[CT]
DraI          112  TTTAAA               TTT^AAA
Eco81I         25  CCTAAGG              CC^T[ACGT]AGG
EcoT14I       371  CCAAGG               C^C[AT][AT]GG
FauI          261  GCGGG                CCCGC(4/6)
FokI          293  CATCC                GGATG(9/13)
HapII         528  CCGG                 C^CGG
HincII        393  GTCAAC               GT[CT]^[GA]AC
HindII        393  GTCAAC               GT[CT]^[GA]AC
HinfI         545  GAGTC                G^A[ACGT]TC
HpaII         528  CCGG                 C^CGG
MseI          113  TTAA                 T^TAA
MslI          418  CACCCTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        328  CAGCTG               C[AC]G^C[GT]G
MspI          528  CCGG                 C^CGG
NlaIII        163  CATG                 CATG^
PinAI         527  ACCGGT               A^CCGGT
PleI          545  GAGTC                GAGTC(4/5)
PvuII         328  CAGCTG               CAG^CTG
ScaI          455  AGTACT               AGT^ACT
StyI          371  CCAAGG               C^C[AT][AT]GG
Tru9I         113  TTAA                 T^TAA
XbaI          124  TCTAGA               T^CTAGA
XmnI          128  GAAAGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AfaI          456  GTAC                 GT^AC
              507  GTAC                
BalI          188  TGGCCA               TGG^CCA
              368  TGGCCA              
BbvI           87  GCAGC                GCAGC(8/12)
               90  GCAGC               
BfaI          125  CTAG                 C^TAG
              521  CTAG                
BsgI          361  GTGCAG               GTGCAG(16/14)
              531  GTGCAG              
BstYI         148  AGATCC               [GA]^GATC[CT]
              264  GGATCC              
DpnII         149  GATC                 ^GATC
              265  GATC                
EaeI          188  TGGCCA               [CT]^GGCC[GA]
              368  TGGCCA              
Fnu4HI         87  GCAGC                GC^[ACGT]GC
               90  GCAGC               
ItaI           87  GCAGC                GC^[ACGT]GC
               90  GCAGC               
MaeI          125  CTAG                 C^TAG
              521  CTAG                
MaeII          47  ACGT                 A^CGT
              104  ACGT                
MboI          149  GATC                 ^GATC
              265  GATC                
MflI          148  AGATCC               [GA]^GATC[CT]
              264  GGATCC              
MluNI         188  TGGCCA               TGG^CCA
              368  TGGCCA              
MscI          188  TGGCCA               TGG^CCA
              368  TGGCCA              
NdeII         149  GATC                 ^GATC
              265  GATC                
NlaIV         264  GGATCC               GG[ACGT]^[ACGT]CC
              273  GGAACC              
RsaI          456  GTAC                 GT^AC
              507  GTAC                
Sau3AI        149  GATC                 ^GATC
              265  GATC                
TaqI          197  TCGA                 T^CGA
              461  TCGA                
TseI           87  GCAGC                G^C[AT]GC
               90  GCAGC               
TthHB8I       197  TCGA                 T^CGA
              461  TCGA                
XhoII         148  AGATCC               [GA]^GATC[CT]
              264  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AluI        AlwI        BsaJI       BssKI       BstNI       
DdeI        EarI        EcoRII      HaeIII      Ksp632I     
MboII       MnlI        MvaI        ScrFI       Tsp509I     
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