Restriction map for pF1KB9786
##################################
# NO CUT ENZYMES (  162 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AcsI        
AflII       AflIII      AgeI        AhdI        AluI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BpmI        BpuAI       
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmAI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DrdI        DsaI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HgaI        HindIII     
HinfI       HpaI        KpnI        Ksp632I     KspI        
MaeI        MaeIII      MamI        MboII       MfeI        
MluI        MroI        MseI        MslI        MspA1I      
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tru9I       
Tsp45I      Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccII         123  CGCG                 CG^CG
AclI          226  AACGTT               AA^CGTT
AlwI          444  GATCC                GGATC(4/5)
AlwNI         427  CAGGCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         526  GTGCTC               G[AT]GC[AT]^C
BalI          434  TGGCCA               TGG^CCA
BanII         359  GAGCCC               G[GA]GC[CT]^C
BbvI          417  GCAGC                GCAGC(8/12)
BcnI          519  CCGGG                CC^[GC]GG
BlpI          470  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      470  GCTCAGC              GC^T[ACGT]AGC
BsaJI         509  CCGAGG               C^C[ACGT][ACGT]GG
BseRI         184  GAGGAG               GAGGAG(10/8)
BsiHKAI       526  GTGCTC               G[AT]GC[AT]^C
BsmFI         167  GGGAC                GGGAC(10/14)
BsmI          466  GAATGC               GAATGC(1/-1)
BsrDI         249  CATTGC               GCAATG(2/0)
BssHII        122  GCGCGC               G^CGCGC
BstUI         123  CGCG                 CG^CG
BstXI         326  CCATCCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         443  AGATCC               [GA]^GATC[CT]
CelII         470  GCTCAGC              GC^T[ACGT]AGC
DraII         350  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII        448  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          434  TGGCCA               [CT]^GGCC[GA]
EcoNI         477  CCTGCAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      350  AGGACCT              [GA]G^G[ACGT]CC[CT]
FauI          175  GCGGG                CCCGC(4/6)
HapII         519  CCGG                 C^CGG
HincII        136  GTTGAC               GT[CT]^[GA]AC
HindII        136  GTTGAC               GT[CT]^[GA]AC
HpaII         519  CCGG                 C^CGG
HphI           28  GGTGA                GGTGA(8/7)
MflI          443  AGATCC               [GA]^GATC[CT]
MluNI         434  TGGCCA               TGG^CCA
MscI          434  TGGCCA               TGG^CCA
MspI          519  CCGG                 C^CGG
MvnI          123  CGCG                 CG^CG
NciI          519  CCGGG                CC^[GC]GG
PflMI         426  CCAGGCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         350  AGGACCT              [GA]G^G[AT]CC[CT]
Psp1406I      226  AACGTT               AA^CGTT
SmlI          385  CTCAAG               C^T[CT][GA]AG
TseI          417  GCAGC                G^C[AT]GC
Tsp509I       115  AATT                 ^AATT
Van91I        426  CCAGGCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          326  CCATCCTGCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         443  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcyI          380  GGCGCC               G[GA]^CG[CT]C
              402  GGCGCC              
AfaI           93  GTAC                 GT^AC
              203  GTAC                
AvaII         351  GGACC                G^G[AT]CC
              557  GGTCC               
BbeI          380  GGCGCC               GGCGC^C
              402  GGCGCC              
BsaHI         380  GGCGCC               G[GA]^CG[CT]C
              402  GGCGCC              
BsrI          432  ACTGG                ACTGG(1/-1)
              254  CCAGT               
BstAPI          8  GCATCAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
               44  GCAAGACGTGC         
DpnII         444  GATC                 ^GATC
              543  GATC                
Fnu4HI        279  GCGGC                GC^[ACGT]GC
              417  GCAGC               
HaeII         380  GGCGCC               [GA]GCGC^[CT]
              402  GGCGCC              
Hin1I         380  GGCGCC               G[GA]^CG[CT]C
              402  GGCGCC              
ItaI          279  GCGGC                GC^[ACGT]GC
              417  GCAGC               
KasI          380  GGCGCC               G^GCGCC
              402  GGCGCC              
MboI          444  GATC                 ^GATC
              543  GATC                
NarI          380  GGCGCC               GG^CGCC
              402  GGCGCC              
NdeII         444  GATC                 ^GATC
              543  GATC                
RsaI           93  GTAC                 GT^AC
              203  GTAC                
Sau3AI        444  GATC                 ^GATC
              543  GATC                
SfoI          380  GGCGCC               GGC^GCC
              402  GGCGCC              
TaqI          110  TCGA                 T^CGA
              464  TCGA                
TthHB8I       110  TCGA                 T^CGA
              464  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AciI        BanI        BmyI        BsiYI       BslI        
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        EcoRII      FokI        
HaeIII      HhaI        HinP1I      MaeII       MnlI        
MvaI        MwoI        NlaIV       Sau96I      ScrFI       
SfaNI       TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com