Restriction map for pF1KB9781
##################################
# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BbvI        BcgI        
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsoBI       BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoT22I     FauI        
FbaI        Fnu4HI      FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HphI        
ItaI        KasI        KpnI        KspI        MaeI        
MaeIII      MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MspA1I      MunI        MvnI        NarI        NcoI        
NdeI        NdeII       NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        Sau3AI      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        TfiI        Tru9I       TseI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AfaI          213  GTAC                 GT^AC
AvaII         237  GGACC                G^G[AT]CC
BanII         122  GAGCCC               G[GA]GC[CT]^C
BbsI          333  GAAGAC               GAAGAC(2/6)
BciVI         163  GTATCC               GTATCC(6/5)
BcnI           40  CCCGG                CC^[GC]GG
BpmI          278  CTGGAG               CTGGAG(16/14)
BpuAI         333  GAAGAC               GAAGAC(2/6)
BseRI          93  GAGGAG               GAGGAG(10/8)
BsmI          115  GAATGC               GAATGC(1/-1)
Bsp1407I      212  TGTACA               T^GTACA
BsrDI         232  GCAATG               GCAATG(2/0)
BsrFI          98  GCCGGC               [GA]^CCGG[CT]
BsrGI         212  TGTACA               T^GTACA
BstF5I          3  GGATG                GGATG(2/0)
BstNI         356  CCTGG                CC^[AT]GG
Cfr10I         98  GCCGGC               [GA]^CCGG[CT]
DraII         266  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoNI         192  CCTCAGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      266  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRII        356  CCTGG                ^CC[AT]GG
EcoRV         140  GATATC               GAT^ATC
FokI            3  GGATG                GGATG(9/13)
HaeIII        267  GGCC                 GG^CC
MaeII          48  ACGT                 A^CGT
MslI          228  CATGGCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          356  CCTGG                CC^[AT]GG
MwoI          181  GCTATATGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI           98  GCCGGC               GCC^GGC
NciI           40  CCCGG                CC^[GC]GG
NgoMIV         98  GCCGGC               G^CCGGC
NlaIII        228  CATG                 CATG^
NlaIV          95  GGAGCC               GG[ACGT]^[ACGT]CC
RsaI          213  GTAC                 GT^AC
SapI          283  GAAGAGC              GCTCTTC(1/4)
SfaNI         340  GATGC                GCATC(5/9)
SspBI         212  TGTACA               T^GTACA
TaqI          171  TCGA                 T^CGA
TthHB8I       171  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AluI          134  AGCT                 AG^CT
              180  AGCT                
BmyI          122  GAGCCC               G[AGT]GC[ACT]^C
              188  GTGCCC              
BsaJI         240  CCAAGG               C^C[ACGT][ACGT]GG
              323  CCAAGG              
BsiYI         192  CCTCAGAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              323  CCAAGGCTGGG         
BslI          192  CCTCAGAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              323  CCAAGGCTGGG         
Bsp1286I      122  GAGCCC               G[AGT]GC[ACT]^C
              188  GTGCCC              
BssKI          40  CCCGG                ^CC[ACGT]GG
              356  CCTGG               
Cfr13I        237  GGACC                G^G[ACGT]CC
              267  GGCCC               
EarI          154  GAAGAG               CTCTTC(1/4)
              283  GAAGAG              
EcoT14I       240  CCAAGG               C^C[AT][AT]GG
              323  CCAAGG              
HapII          41  CCGG                 C^CGG
               99  CCGG                
HpaII          41  CCGG                 C^CGG
               99  CCGG                
Ksp632I       154  GAAGAG               CTCTTC(1/4)
              283  GAAGAG              
MspI           41  CCGG                 C^CGG
               99  CCGG                
Sau96I        237  GGACC                G^G[ACGT]CC
              267  GGCCC               
ScrFI          40  CCCGG                CC^[ACGT]GG
              356  CCTGG               
SmlI           88  CTTGAG               C^T[CT][GA]AG
              309  CTCAAG              
StyI          240  CCAAGG               C^C[AT][AT]GG
              323  CCAAGG              
Tsp509I        69  AATT                 ^AATT
              352  AATT                
TspRI         220  CAGTG                CA[GC]TG[ACGT][ACGT]^
              276  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

BsrI        Cac8I       DdeI        MboII       MnlI        
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