Restriction map for pF1KB9779
 (Circular) (Six-base) MAPSORT of: pF1KB9779.seq  Check: 4994  from: 1  to: 5774



pF1KB9779  5774 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:31  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2540    2540 
   Size:       5774
 
AccI GT'mk_AC

Cuts at:   2812    2812 
   Size:       5774
 
Acc65I G'GTAC_C

Cuts at:   2790    2790 
   Size:       5774
 
AclI AA'CG_TT

Cuts at:   5614    5614 
   Size:       5774
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1445    2201    3599    4031    4384    5046    1445 
   Size:        756    1398     432     353     662    2173

  Fragments arranged by size: 

                2173    1398     756     662     432     353
 
AflII C'TTAA_G

Cuts at:    169     169 
   Size:       5774
 
AflIII A'CryG_T

Cuts at:   3126    4926    5207    3126 
   Size:       1800     281    3693

  Fragments arranged by size: 

                3693    1800     281
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2551    2583    2551 
   Size:         32    5742
 
AlwNI CAG_nnn'CTG

Cuts at:    469    2173    2691    3079    4517     469 
   Size:       1704     518     388    1438    1726

  Fragments arranged by size: 

                1726    1704    1438     518     388
 
ApaLI G'TGCA_C

Cuts at:   4612    4612 
   Size:       5774
 
ApoI r'AATT_y

Cuts at:    766     897    1102    1326    1401    1413    1857    2641    2778 
   Size:        131     205     224      75      12     444     784     137

Cuts at:   2778    5127    5383     766 
   Size:       2349     256    1157

  Fragments arranged by size: 

                2349    1157     784     444     256     224     205     137
                 131      75      12
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5774
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5774
 
AvaI C'yCGr_G

Cuts at:   2794    2794 
   Size:       5774
 
BamHI G'GATC_C

Cuts at:   2412    2799    2412 
   Size:        387    5387
 
BanI G'GyrC_C

Cuts at:   2339    2790    3204    3452    3487    2339 
   Size:        451     414     248      35    4626

  Fragments arranged by size: 

                4626     451     414     248      35
 
BanII G_rGCy'C

Cuts at:   2788    3818    2788 
   Size:       1030    4744
 
BbeI G_GCGC'C

Cuts at:   3208    3456    3208 
   Size:        248    5526
 
BbsI GAAGACnn'nnnn_

Cuts at:    233     233 
   Size:       5774
 
BbvCI CC'TCA_GC

Cuts at:   2264    2264 
   Size:       5774
 
BciVI GTATCCnnnnn_n'

Cuts at:   3672    4728    5774    3672 
   Size:       1056    1046    3672

  Fragments arranged by size: 

                3672    1056    1046
 
BclI T'GATC_A

Cuts at:   2309    3294    2309 
   Size:        985    4789
 
BfrBI ATG'CAT

Cuts at:    528     528 
   Size:       5774
 
BglI GCCn_nnn'nGGC

Cuts at:    424    1147     424 
   Size:        723    5051
 
BglII A'GATC_T

Cuts at:   1047    1527    3289    5326    1047 
   Size:        480    1762    2037    1495

  Fragments arranged by size: 

                2037    1762    1495     480
 
BlpI GC'TnA_GC

Cuts at:   2886    2886 
   Size:       5774
 
Bme1580I G_kGCm'C

Cuts at:   1953    2476    3399    3492    4616    1953 
   Size:        523     923      93    1124    3111

  Fragments arranged by size: 

                3111    1124     923     523      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   2748    2986    3067    3402    5546    2748 
   Size:        238      81     335    2144    2976

  Fragments arranged by size: 

                2976    2144     335     238      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    285    4141     285 
   Size:       3856    1918
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2781    2781 
   Size:       5774
 
Bpu10I CC'TnA_GC

Cuts at:   2264    2264 
   Size:       5774
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1118    2950    3829    4543    4841    1118 
   Size:       1832     879     714     298    2051

  Fragments arranged by size: 

                2051    1832     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   5478    5478 
   Size:       5774
 
BsaAI yAC'GTr

Cuts at:   3127    3127 
   Size:       5774
 
BsaBI GATnn'nnATC

Cuts at:      6    3288    5325       6 
   Size:       3282    2037     455

  Fragments arranged by size: 

                3282    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   3205    3453    4155    5651    3205 
   Size:        248     702    1496    3328

  Fragments arranged by size: 

                3328    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1923    2963    3484    4162    4573    4720    4994    1923 
   Size:       1040     521     678     411     147     274    2703

  Fragments arranged by size: 

                2703    1040     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    567     597     567 
   Size:         30    5744
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1466    1466 
   Size:       5774
 
BseYI C'CCAG_C

Cuts at:   3199    3957    4622    5030    3199 
   Size:        758     665     408    3943

  Fragments arranged by size: 

                3943     758     665     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1721    1946    1721 
   Size:        225    5549
 
BsiEI CG_ry'CG

Cuts at:    104    3316    3362    4592     104 
   Size:       3212      46    1230    1286

  Fragments arranged by size: 

                3212    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   2788    3566    3756    4616    2788 
   Size:        778     190     860    3946

  Fragments arranged by size: 

                3946     860     778     190
 
BsmI GAATG_Cn'

Cuts at:    420    2204     420 
   Size:       1784    3990
 
Bsp1286I G_dGCh'C

Cuts at:   1953    2476    2788    3399    3492    3566    3756    3818    4616 
   Size:        523     312     611      93      74     190      62     798

Cuts at:   4616    1953 
   Size:       3111

  Fragments arranged by size: 

                3111     798     611     523     312     190      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   1923    1923 
   Size:       5774
 
BspHI T'CATG_A

Cuts at:   4206    4206 
   Size:       5774
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    413    2540    2824    3038    3340    3721     413 
   Size:       2127     284     214     302     381    2466

  Fragments arranged by size: 

                2466    2127     381     302     284     214
 
BsrBI CCG'CTC

Cuts at:     50    4066    4120    5604    5769      50 
   Size:       4016      54    1484     165      55

  Fragments arranged by size: 

                4016    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1831    3686    1831 
   Size:       1855    3919
 
BsrFI r'CCGG_y

Cuts at:   3772    3953    3772 
   Size:        181    5593
 
BssSI C'ACGA_G

Cuts at:    981    4045    4753     981 
   Size:       3064     708    2002

  Fragments arranged by size: 

                3064    2002     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2682    3151    2682 
   Size:        469    5305
 
BstBI TT'CG_AA

Cuts at:   4135    4135 
   Size:       5774
 
BstYI r'GATC_y

Cuts at:      1    1047    1125    1527    1635    2412    2799    3266    3289 
   Size:       1046      78     402     108     777     387     467      23

Cuts at:   3289    3624    3870    4176    4188    4274    5326       1 
   Size:        335     246     306      12      86    1052     449

  Fragments arranged by size: 

                1052    1046     777     467     449     402     387     335
                 306     246     108      86      78      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       5774
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3724    3905    4068    3724 
   Size:        181     163    5430

  Fragments arranged by size: 

                5430     181     163
 
BtsI GCAGTG_nn'

Cuts at:    470    2732    3017     470 
   Size:       2262     285    3227

  Fragments arranged by size: 

                3227    2262     285
 
DraI TTT'AAA

Cuts at:   1944    2773    1944 
   Size:        829    4945
 
DraIII CAC_nnn'GTG

Cuts at:    669    2236    2482     669 
   Size:       1567     246    3961

  Fragments arranged by size: 

                3961    1567     246
 
DrdI GACnn_nn'nnGTC

Cuts at:    538    3480    4824     538 
   Size:       2942    1344    1488

  Fragments arranged by size: 

                2942    1488    1344
 
EaeI y'GGCC_r

Cuts at:   3359    3533    3924    3951    5296    3359 
   Size:        174     391      27    1345    3837

  Fragments arranged by size: 

                3837    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   3359    3359 
   Size:       5774
 
EarI CTCTTCn'nnn_

Cuts at:    187    1473    2383    2429    2660    3797    4007     187 
   Size:       1286     910      46     231    1137     210    1954

  Fragments arranged by size: 

                1954    1286    1137     910     231     210      46
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3982    4724    4870    5584    3982 
   Size:        742     146     714    4172

  Fragments arranged by size: 

                4172     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   2786    2786 
   Size:       5774
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1445    2201    2781    3599    4031    4384    5046    1445 
   Size:        756     580     818     432     353     662    2173

  Fragments arranged by size: 

                2173     818     756     662     580     432     353
 
EcoNI CCTnn'n_nnAGG

Cuts at:    473     473 
   Size:       5774
 
EcoO109I rG'GnC_Cy

Cuts at:   2913    2913 
   Size:       5774
 
EcoRI G'AATT_C

Cuts at:   2778    2778 
   Size:       5774
 
EcoRV GAT'ATC

Cuts at:    156    1547     156 
   Size:       1391    4383
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    965    2059    3793     965 
   Size:       1094    1734    2946

  Fragments arranged by size: 

                2946    1734    1094
 
FspI TGC'GCA

Cuts at:   3555    3555 
   Size:       5774
 
HaeII r_GCGC'y

Cuts at:   3208    3456    4686    5457    3208 
   Size:        248    1230     771    3525

  Fragments arranged by size: 

                3525    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    612     644     820     852    1192    1224    3616    3646    3648 
   Size:         32     176      32     340      32    2392      30       2

Cuts at:   3648    3678     612 
   Size:         30    2708

  Fragments arranged by size: 

                2708    2392     340     176      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   2813    2813 
   Size:       5774
 
HindIII A'AGCT_T

Cuts at:    180     922     180 
   Size:        742    5032
 
KasI G'GCGC_C

Cuts at:   3204    3452    3204 
   Size:        248    5526
 
KpnI G_GTAC'C

Cuts at:   2794    2794 
   Size:       5774
 
MfeI C'AATT_G

Cuts at:   1300    1300 
   Size:       5774
 
MluI A'CGCG_T

Cuts at:   5207    5207 
   Size:       5774
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2310    2639    4533    4717    2310 
   Size:        329    1894     184    3367

  Fragments arranged by size: 

                3367    1894     329     184
 
MscI TGG'CCA

Cuts at:   3535    3535 
   Size:       5774
 
MslI CAynn'nnrTG

Cuts at:   2021    3763    3890    2021 
   Size:       1742     127    3905

  Fragments arranged by size: 

                3905    1742     127
 
MspA1I CmG'CkG

Cuts at:   2885    3199    3559    4343    4588    2885 
   Size:        314     360     784     245    4071

  Fragments arranged by size: 

                4071     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   3955    3955 
   Size:       5774
 
NarI GG'CG_CC

Cuts at:   3205    3453    3205 
   Size:        248    5526
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       5774
 
NdeI CA'TA_TG

Cuts at:    436    1035     436 
   Size:        599    5175
 
NgoMIV G'CCGG_C

Cuts at:   3953    3953 
   Size:       5774
 
NsiI A_TGCA'T

Cuts at:    530     530 
   Size:       5774
 
NspI r_CATG'y

Cuts at:    269     513    2685    2827    3327    4930     269 
   Size:        244    2172     142     500    1603    1113

  Fragments arranged by size: 

                2172    1603    1113     500     244     142
 
PciI A'CATG_T

Cuts at:   4926    4926 
   Size:       5774
 
PfoI T'CCnGG_A

Cuts at:    221     453     221 
   Size:        232    5542
 
PmeI GTTT'AAAC

Cuts at:   2773    2773 
   Size:       5774
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1514    1546    4220    4252    1514 
   Size:         32    2674      32    3036

  Fragments arranged by size: 

                3036    2674      32      32
 
PsiI TTA'TAA

Cuts at:    212    1444    2117     212 
   Size:       1232     673    3869

  Fragments arranged by size: 

                3869    1232     673
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   2482    2514    2482 
   Size:         32    5742
 
PstI C_TGCA'G

Cuts at:   1422    2727    2821    3506    1422 
   Size:       1305      94     685    3690

  Fragments arranged by size: 

                3690    1305     685      94
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5774
 
PvuII CAG'CTG

Cuts at:   3199    3559    3199 
   Size:        360    5414
 
RsrII CG'GwC_CG

Cuts at:   3969    3969 
   Size:       5774
 
SacI G_AGCT'C

Cuts at:   2788    2788 
   Size:       5774
 
SalI G'TCGA_C

Cuts at:   2811    2811 
   Size:       5774
 
SapI GCTCTTCn'nnn_

Cuts at:   3797    4007    3797 
   Size:        210    5564
 
SbfI CC_TGCA'GG

Cuts at:   2821    2821 
   Size:       5774
 
ScaI AGT'ACT

Cuts at:   2758    5291    2758 
   Size:       2533    3241
 
SfcI C'TryA_G

Cuts at:     33     138     470    1418    1825    2723    2817    3502    4470 
   Size:        105     332     948     407     898      94     685     968

Cuts at:   4470    4661      33 
   Size:        191    1146

  Fragments arranged by size: 

                1146     968     948     898     685     407     332     191
                 105      94
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1147    1147 
   Size:       5774
 
SfoI GGC'GCC

Cuts at:   3206    3454    3206 
   Size:        248    5526
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5774
 
SmaI CCC'GGG

Cuts at:   2796    2796 
   Size:       5774
 
SmlI C'TyrA_G

Cuts at:    169    1133    2929    3844    4558    4820     169 
   Size:        964    1796     915     714     262    1123

  Fragments arranged by size: 

                1796    1123     964     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5774
 
SphI G_CATG'C

Cuts at:   2827    3327    2827 
   Size:        500    5274
 
SspI AAT'ATT

Cuts at:    999     999 
   Size:       5774
 
StuI AGG'CCT

Cuts at:   1150    1150 
   Size:       5774
 
StyI C'CwwG_G

Cuts at:    107     519    1151    1196    2908    3262     107 
   Size:        412     632      45    1712     354    2619

  Fragments arranged by size: 

                2619    1712     632     412     354      45
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   3357    3490    3949    4158    3357 
   Size:        133     459     209    4973

  Fragments arranged by size: 

                4973     459     209     133
 
TatI w'GTAC_w

Cuts at:    352    1655    1782    2134    2756    5289     352 
   Size:       1303     127     352     622    2533     837

  Fragments arranged by size: 

                2533    1303     837     622     352     127
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    449     481     567     599     653     685    2291    2323    5291 
   Size:         32      86      32      54      32    1606      32    2968

Cuts at:   5291    5323     449 
   Size:         32     900

  Fragments arranged by size: 

                2968    1606     900      86      54      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3571    3571 
   Size:       5774
 
XbaI T'CTAG_A

Cuts at:   2805    2805 
   Size:       5774
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1149    2389    3269    1149 
   Size:       1240     880    3654

  Fragments arranged by size: 

                3654    1240     880
 
XmaI C'CCGG_G

Cuts at:   2794    2794 
   Size:       5774
 
XmnI GAAnn'nnTTC

Cuts at:   1413    2541    2738    1413 
   Size:       1128     197    4449

  Fragments arranged by size: 

                4449    1128     197
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AloI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NsiI     NspI     PciI     PfoI     PmeI     PpiI
     PsiI     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI     AgeI     AhdI     AleI     AlfI     ApaI     AscI
    AvrII     BaeI     BcgI    BmgBI     BmtI    BsiWI    BsmBI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     FseI
    FspAI     HpaI     NheI     NotI     NruI     PacI     PasI    PflMI
     PmlI    PpuMI    PshAI   PspOMI    PspXI    SacII    SanDI    SexAI
    SgrAI    SnaBI     SrfI     SwaI     XhoI     ZraI
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