Restriction map for
pF1KB9778
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BfrI BglI BlnI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI BsoBI BspDI
BspEI BspLU11I BsrBI BssHII BssSI
BstBI BstEII BstPI Bsu36I ClaI
CpoI DraII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FauI FseI FspI HaeII Hin1I
KasI KpnI KspI MluI MluNI
MroI MscI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SphI Sse8387I SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 1461 CGCG CG^CG
AciI 1598 GCGG CCGC(-3/-1)
AflIII 1885 ACGTGT A^C[GA][CT]GT
Alw44I 141 GTGCAC G^TGCAC
ApaLI 141 GTGCAC G^TGCAC
AspI 896 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 1782 GGATAC GTATCC(6/5)
BsaAI 2109 TACGTA [CT]AC^GT[GA]
BsaBI 365 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 519 GAGGAG GAGGAG(10/8)
BsrFI 2043 GCCGGT [GA]^CCGG[CT]
Bst1107I 113 GTATAC GTA^TAC
BstAPI 1649 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 906 CCTGG CC^[AT]GG
BstUI 1461 CGCG CG^CG
BstXI 13 CCAATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 113 GTATAC GTA^TAC
Cfr10I 2043 GCCGGT [GA]^CCGG[CT]
DraIII 185 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1824 TGGCCG [CT]^GGCC[GA]
EarI 1617 GAAGAG CTCTTC(1/4)
EcoRII 906 CCTGG ^CC[AT]GG
EcoT22I 1118 ATGCAT ATGCA^T
HgaI 1592 GCGTC GACGC(5/10)
HindIII 2254 AAGCTT A^AGCTT
HpaI 1519 GTTAAC GTT^AAC
Ksp632I 1617 GAAGAG CTCTTC(1/4)
MamI 365 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2192 CAATTG C^AATTG
MunI 2192 CAATTG C^AATTG
MvaI 906 CCTGG CC^[AT]GG
MvnI 1461 CGCG CG^CG
NsiI 1118 ATGCAT ATGCA^T
PflFI 896 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 1789 AGTACT AGT^ACT
SmlI 316 CTCAAG C^T[CT][GA]AG
SnaBI 2109 TACGTA TAC^GTA
SpeI 1786 ACTAGT A^CTAGT
StuI 1336 AGGCCT AGG^CCT
TaqI 755 TCGA T^CGA
Tth111I 896 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 755 TCGA T^CGA
XcmI 127 CCAAATTGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
AccI 113 GTATAC GT^[AC][GT]AC
1490 GTAGAC
AlwNI 970 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
1007 CAGTTACTG
AspHI 141 GTGCAC G[AT]GC[AT]^C
664 GTGCTC
BbsI 415 GAAGAC GAAGAC(2/6)
1528 GAAGAC
BbvI 1505 GCAGC GCAGC(8/12)
816 GCTGC
BclI 32 TGATCA T^GATCA
1109 TGATCA
BcnI 839 CCGGG CC^[GC]GG
1827 CCGGG
BfaI 1091 CTAG C^TAG
1787 CTAG
BglII 501 AGATCT A^GATCT
1814 AGATCT
BlpI 601 GCTAAGC GC^T[ACGT]AGC
1921 GCTGAGC
BmyI 141 GTGCAC G[AGT]GC[ACT]^C
664 GTGCTC
Bpu1102I 601 GCTAAGC GC^T[ACGT]AGC
1921 GCTGAGC
BpuAI 415 GAAGAC GAAGAC(2/6)
1528 GAAGAC
BsiHKAI 141 GTGCAC G[AT]GC[AT]^C
664 GTGCTC
Bsp1286I 141 GTGCAC G[AGT]GC[ACT]^C
664 GTGCTC
Bsp1407I 920 TGTACA T^GTACA
1858 TGTACA
BspHI 1431 TCATGA T^CATGA
2271 TCATGA
BspMI 1903 ACCTGC ACCTGC(4/8)
1466 GCAGGT
BsrGI 920 TGTACA T^GTACA
1858 TGTACA
BsrI 1756 ACTGG ACTGG(1/-1)
2173 ACTGG
Cac8I 1462 GCGTGC GC[ACGT]^[ACGT]GC
1801 GCCAGC
CelII 601 GCTAAGC GC^T[ACGT]AGC
1921 GCTGAGC
CfoI 717 GCGC GCG^C
1460 GCGC
DraI 81 TTTAAA TTT^AAA
1327 TTTAAA
EcoT14I 1908 CCTTGG C^C[AT][AT]GG
2012 CCAAGG
FbaI 32 TGATCA T^GATCA
1109 TGATCA
Fnu4HI 816 GCTGC GC^[ACGT]GC
1505 GCAGC
HhaI 717 GCGC GCG^C
1460 GCGC
HinP1I 717 GCGC G^CGC
1460 GCGC
HincII 1519 GTTAAC GT[CT]^[GA]AC
2086 GTTGAC
HindII 1519 GTTAAC GT[CT]^[GA]AC
2086 GTTGAC
ItaI 816 GCTGC GC^[ACGT]GC
1505 GCAGC
MaeI 1091 CTAG C^TAG
1787 CTAG
MaeII 1885 ACGT A^CGT
2110 ACGT
MwoI 644 GCCCATTTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1649 GCAAATTCTGC
NciI 839 CCGGG CC^[GC]GG
1827 CCGGG
NspI 1765 GCATGT [GA]CATG^[CT]
1978 ACATGC
PstI 1545 CTGCAG CTGCA^G
1572 CTGCAG
RcaI 1431 TCATGA T^CATGA
2271 TCATGA
SfcI 1545 CTGCAG C^T[GA][CT]AG
1572 CTGCAG
SspBI 920 TGTACA T^GTACA
1858 TGTACA
SspI 988 AATATT AAT^ATT
1249 AATATT
StyI 1908 CCTTGG C^C[AT][AT]GG
2012 CCAAGG
TseI 816 GCTGC G^C[AT]GC
1505 GCAGC
Tsp45I 902 GTCAC ^GT[GC]AC
1769 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AvaII BpmI BsaJI BsgI
BsiYI BslI BsmFI BsmI BsrDI
BssKI BstF5I BstYI Cfr13I DdeI
DpnII FokI HaeIII HapII HinfI
HpaII HphI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
NdeII NlaIII NlaIV PflMI RsaI
Sau3AI Sau96I ScrFI SfaNI TfiI
Tru9I Tsp509I TspRI Van91I XhoII
XmnI