Restriction map for pF1KB9778
 (Linear) (Six-base) MAPSORT of: KIBB9778.seq  Check: 3850  from: 1  to: 2394



KIBB9778.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1457    2394 
   Size:       1457     937
 
AccI GT'mk_AC

Cuts at:      0     114    1491    2394 
   Size:        114    1377     903

  Fragments arranged by size: 

                1377     903     114
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     434     522    1034    1357    2394 
   Size:        434      88     512     323    1037

  Fragments arranged by size: 

                1037     512     434     323      88
 
AflIII A'CryG_T

Cuts at:      0    1885    2394 
   Size:       1885     509
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1253    1285    2394 
   Size:       1253      32    1109

  Fragments arranged by size: 

                1253    1109      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     975    1012    2394 
   Size:        975      37    1382

  Fragments arranged by size: 

                1382     975      37
 
ApaLI G'TGCA_C

Cuts at:      0     141    2394 
   Size:        141    2253
 
ApoI r'AATT_y

Cuts at:      0       3     147     400     729    1018    1637    1651    2293 
   Size:          3     144     253     329     289     619      14     642

Cuts at:   2293    2394 
   Size:        101

  Fragments arranged by size: 

                 642     619     329     289     253     144     101      14
                   3
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     422    1535    2394 
   Size:        422    1113     859

  Fragments arranged by size: 

                1113     859     422
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1776    2394 
   Size:       1776     618
 
BclI T'GATC_A

Cuts at:      0      32    1109    2394 
   Size:         32    1077    1285

  Fragments arranged by size: 

                1285    1077      32
 
BfrBI ATG'CAT

Cuts at:      0    1120    2394 
   Size:       1120    1274
 
BglII A'GATC_T

Cuts at:      0     501    1814    2394 
   Size:        501    1313     580

  Fragments arranged by size: 

                1313     580     501
 
BlpI GC'TnA_GC

Cuts at:      0     602    1922    2394 
   Size:        602    1320     472

  Fragments arranged by size: 

                1320     602     472
 
Bme1580I G_kGCm'C

Cuts at:      0     145    2394 
   Size:        145    2249
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     725     819     875    2394 
   Size:        212     513      94      56    1519

  Fragments arranged by size: 

                1519     513     212      94      56
 
Bpu10I CC'TnA_GC

Cuts at:      0     607    2394 
   Size:        607    1787
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301    2394 
   Size:        301    2093
 
BsaAI yAC'GTr

Cuts at:      0    2111    2394 
   Size:       2111     283
 
BsaBI GATnn'nnATC

Cuts at:      0     369    2394 
   Size:        369    2025
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1253    1283    1577    1607    2394 
   Size:       1253      30     294      30     787

  Fragments arranged by size: 

                1253     787     294      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     534    2394 
   Size:        534    1860
 
BseYI C'CCAG_C

Cuts at:      0    2039    2394 
   Size:       2039     355
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     621    1485    1965    2394 
   Size:        167     454     864     480     429

  Fragments arranged by size: 

                 864     480     454     429     167
 
BsiHKAI G_wGCw'C

Cuts at:      0     145     668    2394 
   Size:        145     523    1726

  Fragments arranged by size: 

                1726     523     145
 
BsmI GAATG_Cn'

Cuts at:      0     176    1399    1507    2319    2394 
   Size:        176    1223     108     812      75

  Fragments arranged by size: 

                1223     812     176     108      75
 
Bsp1286I G_dGCh'C

Cuts at:      0     145     668    2394 
   Size:        145     523    1726

  Fragments arranged by size: 

                1726     523     145
 
BspHI T'CATG_A

Cuts at:      0    1431    2271    2394 
   Size:       1431     840     123

  Fragments arranged by size: 

                1431     840     123
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1457    1912    2394 
   Size:       1457     455     482

  Fragments arranged by size: 

                1457     482     455
 
BsrDI GCAATG_nn'

Cuts at:      0    1101    1479    1827    2394 
   Size:       1101     378     348     567

  Fragments arranged by size: 

                1101     567     378     348
 
BsrFI r'CCGG_y

Cuts at:      0    2043    2394 
   Size:       2043     351
 
BsrGI T'GTAC_A

Cuts at:      0     920    1858    2394 
   Size:        920     938     536

  Fragments arranged by size: 

                 938     920     536
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1655    2394 
   Size:       1655     739
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      20    2394 
   Size:         20    2374
 
BstYI r'GATC_y

Cuts at:      0     338     501    1684    1814    1863    2394 
   Size:        338     163    1183     130      49     531

  Fragments arranged by size: 

                1183     531     338     163     130      49
 
BstZ17I GTA'TAC

Cuts at:      0     115    2394 
   Size:        115    2279
 
BtsI GCAGTG_nn'

Cuts at:      0    1767    2394 
   Size:       1767     627
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      84     119     335     370    2394 
   Size:         84      35     216      35    2024

  Fragments arranged by size: 

                2024     216      84      35      35
 
DraI TTT'AAA

Cuts at:      0      83    1329    2394 
   Size:         83    1246    1065

  Fragments arranged by size: 

                1246    1065      83
 
DraIII CAC_nnn'GTG

Cuts at:      0     190    2394 
   Size:        190    2204
 
EaeI y'GGCC_r

Cuts at:      0    1824    2394 
   Size:       1824     570
 
EarI CTCTTCn'nnn_

Cuts at:      0    1612    2394 
   Size:       1612     782
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     434     522     725     819     875    1034    1357 
   Size:        212     222      88     203      94      56     159     323

Cuts at:   1357    2394 
   Size:       1037

  Fragments arranged by size: 

                1037     323     222     212     203     159      94      88
                  56
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1577    1609    2047    2079    2394 
   Size:       1577      32     438      32     315

  Fragments arranged by size: 

                1577     438     315      32      32
 
HincII GTy'rAC

Cuts at:      0    1521    2088    2394 
   Size:       1521     567     306

  Fragments arranged by size: 

                1521     567     306
 
HindIII A'AGCT_T

Cuts at:      0    2254    2394 
   Size:       2254     140
 
HpaI GTT'AAC

Cuts at:      0    1521    2394 
   Size:       1521     873
 
MfeI C'AATT_G

Cuts at:      0    2192    2394 
   Size:       2192     202
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     366     687    2073    2394 
   Size:        366     321    1386     321

  Fragments arranged by size: 

                1386     366     321     321
 
MslI CAynn'nnrTG

Cuts at:      0     938    1452    1989    2394 
   Size:        938     514     537     405

  Fragments arranged by size: 

                 938     537     514     405
 
NsiI A_TGCA'T

Cuts at:      0    1122    2394 
   Size:       1122    1272
 
NspI r_CATG'y

Cuts at:      0    1769    1982    2394 
   Size:       1769     213     412

  Fragments arranged by size: 

                1769     412     213
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     133     748     892    2394 
   Size:        133     615     144    1502

  Fragments arranged by size: 

                1502     615     144     133
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1253    1285    2394 
   Size:       1253      32    1109

  Fragments arranged by size: 

                1253    1109      32
 
PsiI TTA'TAA

Cuts at:      0    2348    2394 
   Size:       2348      46
 
PstI C_TGCA'G

Cuts at:      0    1549    1576    2394 
   Size:       1549      27     818

  Fragments arranged by size: 

                1549     818      27
 
ScaI AGT'ACT

Cuts at:      0    1791    2394 
   Size:       1791     603
 
SfcI C'TryA_G

Cuts at:      0    1545    1572    2394 
   Size:       1545      27     822

  Fragments arranged by size: 

                1545     822      27
 
SmlI C'TyrA_G

Cuts at:      0     316    2394 
   Size:        316    2078
 
SnaBI TAC'GTA

Cuts at:      0    2111    2394 
   Size:       2111     283
 
SpeI A'CTAG_T

Cuts at:      0    1786    2394 
   Size:       1786     608
 
SspI AAT'ATT

Cuts at:      0     990    1251    2394 
   Size:        990     261    1143

  Fragments arranged by size: 

                1143     990     261
 
StuI AGG'CCT

Cuts at:      0    1338    2394 
   Size:       1338    1056
 
StyI C'CwwG_G

Cuts at:      0    1908    2012    2394 
   Size:       1908     104     382

  Fragments arranged by size: 

                1908     382     104
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     125    2394 
   Size:        125    2269
 
TatI w'GTAC_w

Cuts at:      0     920    1376    1789    1858    2394 
   Size:        920     456     413      69     536

  Fragments arranged by size: 

                 920     536     456     413      69
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     855     887    2394 
   Size:        855      32    1507

  Fragments arranged by size: 

                1507     855      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     899    2394 
   Size:        899    1495
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     134    2394 
   Size:        134    2260
 
XmnI GAAnn'nnTTC

Cuts at:      0    1019    1242    1937    2394 
   Size:       1019     223     695     457

  Fragments arranged by size: 

                1019     695     457     223
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AloI    AlwNI    ApaLI     ApoI
     BbsI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I     BpmI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI
   BstAPI    BstXI    BstYI  BstZ17I     BtsI    CspCI     DraI   DraIII
     EaeI     EarI  Eco57MI    Hin4I   HincII  HindIII     HpaI     MfeI
     MmeI     MslI     NsiI     NspI    PflMI     PpiI     PsiI     PstI
     ScaI     SfcI     SmlI    SnaBI     SpeI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI     BglI    BmgBI
     BmrI     BmtI     BplI     BsaI    BsaHI    BsaWI    BsiEI    BsiWI
    BsmBI    BspEI    BsrBI   BssHII    BssSI    BstBI   BstEII   Bsu36I
     BtgI    BtgZI     ClaI     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
     KasI     KpnI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
     PfoI     PmeI     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SphI
     SrfI     SwaI     XbaI     XhoI     XmaI     ZraI
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