Restriction map for pF1KB9776
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AlwI        
AlwNI       AocI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AviII       
AvrII       BamHI       BcgI        BciVI       BclI        
BfrI        BlnI        BlpI        Bpu1102I    BseAI       
BsgI        BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstZ17I     Bsu36I      
CelII       ClaI        DraI        EagI        EarI        
EclXI       Eco52I      Eco81I      EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KpnI        Ksp632I     MfeI        MluI        
MroI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshBI       Psp1406I    
SacI        SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tsp509I     Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AhdI         1783  GACTCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        967  GTGCAC               G^TGCAC
Aor51HI      1868  AGCGCT               AGC^GCT
ApaLI         967  GTGCAC               G^TGCAC
AspEI        1783  GACTCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI        1022  CACGTG               CAC^GTG
BbsI          644  GAAGAC               GAAGAC(2/6)
BbvCI         241  GCTGAGG              CCTCAGC(-5/-2)
BfaI         1201  CTAG                 C^TAG
BglI         1053  GCCCATCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         176  AGATCT               A^GATCT
BpuAI         644  GAAGAC               GAAGAC(2/6)
BsaAI        1022  CACGTG               [CT]AC^GT[GA]
BsaBI        1305  GATTACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1379  ACCGGA               [AT]^CCGG[AT]
BsiEI        1749  CGATCG               CG[GA][CT]^CG
BspHI         842  TCATGA               T^CATGA
BsrDI        1037  CATTGC               GCAATG(2/0)
BstEII       1483  GGTGACC              G^GT[ACGT]ACC
BstPI        1483  GGTGACC              G^GT[ACGT]ACC
BstXI          24  CCACTTGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         176  AGATCT               [GA]^GATC[CT]
CpoI         1376  CGGACCG              CG^G[AT]CCG
DraIII       1357  CACAATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          937  GACGATGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1783  GACTCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1868  AGCGCT               AGC^GCT
EcoNI        1264  CCTGAATCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1483  GGTGACC              G^GT[ACGT]ACC
HincII       1441  GTCAAC               GT[CT]^[GA]AC
HindII       1441  GTCAAC               GT[CT]^[GA]AC
MaeI         1201  CTAG                 C^TAG
MaeII        1023  ACGT                 A^CGT
MamI         1305  GATTACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          176  AGATCT               [GA]^GATC[CT]
MseI           98  TTAA                 T^TAA
NaeI         1878  GCCGGC               GCC^GGC
NgoMIV       1878  GCCGGC               G^CCGGC
PmaCI        1022  CACGTG               CAC^GTG
PmlI         1022  CACGTG               CAC^GTG
PshAI        1012  GACCCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1094  CTGCAG               CTGCA^G
PvuI         1749  CGATCG               CGAT^CG
PvuII         239  CAGCTG               CAG^CTG
RcaI          842  TCATGA               T^CATGA
RsrII        1376  CGGACCG              CG^G[AT]CCG
SmlI         1300  CTCAAG               C^T[CT][GA]AG
SphI          459  GCATGC               GCATG^C
TaqI          878  TCGA                 T^CGA
Tru9I          98  TTAA                 T^TAA
TthHB8I       878  TCGA                 T^CGA
XhoII         176  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

ApaI          979  GGGCCC               GGGCC^C
             2029  GGGCCC              
BbeI         1540  GGCGCC               GGCGC^C
             1857  GGCGCC              
BpmI         1533  CTCCAG               CTGGAG(16/14)
              550  CTGGAG              
BseRI        1164  CTCCTC               GAGGAG(10/8)
              172  GAGGAG              
BsmBI          40  GAGACG               CGTCTC(1/5)
              400  GAGACG              
BspMI        1409  ACCTGC               ACCTGC(4/8)
              596  GCAGGT              
BsrFI        1105  ACCGGC               [GA]^CCGG[CT]
             1878  GCCGGC              
Cfr10I       1105  ACCGGC               [GA]^CCGG[CT]
             1878  GCCGGC              
KasI         1540  GGCGCC               G^GCGCC
             1857  GGCGCC              
KspI         1846  CCGCGG               CCGC^GG
             2018  CCGCGG              
NarI         1540  GGCGCC               GG^CGCC
             1857  GGCGCC              
NspI          459  GCATGC               [GA]CATG^[CT]
             1292  GCATGT              
PpuMI        1010  GGGACCC              [GA]G^G[AT]CC[CT]
             1766  AGGACCC             
SacII        1846  CCGCGG               CCGC^GG
             2018  CCGCGG              
SfoI         1540  GGCGCC               GGC^GCC
             1857  GGCGCC              
SmaI         1578  CCCGGG               CCC^GGG
             1650  CCCGGG              
TfiI         1267  GAATC                G^A[AT]TC
             1810  GAATC               
XcmI          351  CCACCCTCATGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1518  CCACGATCACGATGG     
XmaCI        1578  CCCGGG               C^CCGGG
             1650  CCCGGG              
XmaI         1578  CCCGGG               C^CCGGG
             1650  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbvI        BcnI        BmyI        BsaHI       
BsaI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrBI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PflMI       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TseI        Tsp45I      TspRI       Van91I      
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