Restriction map for pF1KB9770
 (Linear) (Six-base) MAPSORT of: KIBB9770.seq  Check: 9200  from: 1  to: 1386



KIBB9770.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     517    1386 
   Size:        517     869
 
AccI GT'mk_AC

Cuts at:      0     664    1386 
   Size:        664     722
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     243    1386 
   Size:        243    1143
 
AgeI A'CCGG_T

Cuts at:      0     554    1386 
   Size:        554     832
 
AleI CACnn'nnGTG

Cuts at:      0      40     835    1386 
   Size:         40     795     551

  Fragments arranged by size: 

                 795     551      40
 
ApaI G_GGCC'C

Cuts at:      0     187     220    1386 
   Size:        187      33    1166

  Fragments arranged by size: 

                1166     187      33
 
AvaI C'yCGr_G

Cuts at:      0     129    1386 
   Size:        129    1257
 
BamHI G'GATC_C

Cuts at:      0     701     854     968    1386 
   Size:        701     153     114     418

  Fragments arranged by size: 

                 701     418     153     114
 
BanI G'GyrC_C

Cuts at:      0     234     557    1027    1368    1386 
   Size:        234     323     470     341      18

  Fragments arranged by size: 

                 470     341     323     234      18
 
BanII G_rGCy'C

Cuts at:      0     166     187     220     731    1148    1289    1386 
   Size:        166      21      33     511     417     141      97

  Fragments arranged by size: 

                 511     417     166     141      97      33      21
 
BbeI G_GCGC'C

Cuts at:      0    1031    1372    1386 
   Size:       1031     341      14

  Fragments arranged by size: 

                1031     341      14
 
BbvCI CC'TCA_GC

Cuts at:      0    1174    1386 
   Size:       1174     212
 
BglI GCCn_nnn'nGGC

Cuts at:      0     341    1386 
   Size:        341    1045
 
BlpI GC'TnA_GC

Cuts at:      0     280     713    1247    1386 
   Size:        280     433     534     139

  Fragments arranged by size: 

                 534     433     280     139
 
Bme1580I G_kGCm'C

Cuts at:      0     187     220     239    1386 
   Size:        187      33      19    1147

  Fragments arranged by size: 

                1147     187      33      19
 
BmgBI CAC'GTC

Cuts at:      0     993    1386 
   Size:        993     393
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     727    1384    1386 
   Size:        727     657       2

  Fragments arranged by size: 

                 727     657       2
 
Bpu10I CC'TnA_GC

Cuts at:      0     160    1174    1386 
   Size:        160    1014     212

  Fragments arranged by size: 

                1014     212     160
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334    1386 
   Size:        334    1052
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     733    1386 
   Size:        733     653
 
BsaAI yAC'GTr

Cuts at:      0     747    1386 
   Size:        747     639
 
BsaHI Gr'CG_yC

Cuts at:      0     344    1028    1369    1386 
   Size:        344     684     341      17

  Fragments arranged by size: 

                 684     344     341      17
 
BsaWI w'CCGG_w

Cuts at:      0     554     641     998    1386 
   Size:        554      87     357     388

  Fragments arranged by size: 

                 554     388     357      87
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1045    1075    1120    1150    1386 
   Size:       1045      30      45      30     236

  Fragments arranged by size: 

                1045     236      45      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      39     414     634    1386 
   Size:         39     375     220     752

  Fragments arranged by size: 

                 752     375     220      39
 
BseYI C'CCAG_C

Cuts at:      0     275     772    1098    1386 
   Size:        275     497     326     288

  Fragments arranged by size: 

                 497     326     288     275
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     634     973    1386 
   Size:         82     552     339     413

  Fragments arranged by size: 

                 552     413     339      82
 
BsiEI CG_ry'CG

Cuts at:      0    1160    1386 
   Size:       1160     226
 
BsiHKAI G_wGCw'C

Cuts at:      0     166    1386 
   Size:        166    1220
 
BsmI GAATG_Cn'

Cuts at:      0     141    1386 
   Size:        141    1245
 
Bsp1286I G_dGCh'C

Cuts at:      0     166     187     220     239     731    1148    1289    1386 
   Size:        166      21      33      19     492     417     141      97

  Fragments arranged by size: 

                 492     417     166     141      97      33      21      19
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     517    1386 
   Size:        517     869
 
BsrBI CCG'CTC

Cuts at:      0      24     422     624     947    1386 
   Size:         24     398     202     323     439

  Fragments arranged by size: 

                 439     398     323     202      24
 
BsrFI r'CCGG_y

Cuts at:      0     554     690    1266    1386 
   Size:        554     136     576     120

  Fragments arranged by size: 

                 576     554     136     120
 
BsrGI T'GTAC_A

Cuts at:      0     446    1386 
   Size:        446     940
 
BssHII G'CGCG_C

Cuts at:      0    1110    1386 
   Size:       1110     276
 
BstYI r'GATC_y

Cuts at:      0     701     854     968    1386 
   Size:        701     153     114     418

  Fragments arranged by size: 

                 701     418     153     114
 
Bsu36I CC'TnA_GG

Cuts at:      0     369     426    1386 
   Size:        369      57     960

  Fragments arranged by size: 

                 960     369      57
 
BtgI C'CryG_G

Cuts at:      0      80     212     590     722    1386 
   Size:         80     132     378     132     664

  Fragments arranged by size: 

                 664     378     132     132      80
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     912     936    1386 
   Size:        912      24     450

  Fragments arranged by size: 

                 912     450      24
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     889    1386 
   Size:        889     497
 
EaeI y'GGCC_r

Cuts at:      0     587     719     977    1157    1386 
   Size:        587     132     258     180     229

  Fragments arranged by size: 

                 587     258     229     180     132
 
EagI C'GGCC_G

Cuts at:      0    1157    1386 
   Size:       1157     229
 
EarI CTCTTCn'nnn_

Cuts at:      0    1312    1386 
   Size:       1312      74
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1249    1386 
   Size:       1249     137
 
EcoICRI GAG'CTC

Cuts at:      0     164    1386 
   Size:        164    1222
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     243     727    1384    1386 
   Size:        243     484     657       2

  Fragments arranged by size: 

                 657     484     243       2
 
EcoO109I rG'GnC_Cy

Cuts at:      0     112     217     354     495    1386 
   Size:        112     105     137     141     891

  Fragments arranged by size: 

                 891     141     137     112     105
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     489    1386 
   Size:        489     897
 
FspI TGC'GCA

Cuts at:      0     490    1386 
   Size:        490     896
 
HaeII r_GCGC'y

Cuts at:      0     677    1010    1031    1174    1372    1386 
   Size:        677     333      21     143     198      14

  Fragments arranged by size: 

                 677     333     198     143      21      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     409     441     646     678    1045    1077    1386 
   Size:        409      32     205      32     367      32     309

  Fragments arranged by size: 

                 409     367     309     205      32      32      32
 
HincII GTy'rAC

Cuts at:      0     665    1386 
   Size:        665     721
 
KasI G'GCGC_C

Cuts at:      0    1027    1368    1386 
   Size:       1027     341      18

  Fragments arranged by size: 

                1027     341      18
 
MscI TGG'CCA

Cuts at:      0     589     979    1386 
   Size:        589     390     407

  Fragments arranged by size: 

                 589     407     390
 
MslI CAynn'nnrTG

Cuts at:      0      40     232     595     691     835     955    1081    1386 
   Size:         40     192     363      96     144     120     126     305

  Fragments arranged by size: 

                 363     305     192     144     126     120      96      40
            
 
MspA1I CmG'CkG

Cuts at:      0     138     452     549    1386 
   Size:        138     314      97     837

  Fragments arranged by size: 

                 837     314     138      97
 
NaeI GCC'GGC

Cuts at:      0    1268    1386 
   Size:       1268     118
 
NarI GG'CG_CC

Cuts at:      0    1028    1369    1386 
   Size:       1028     341      17

  Fragments arranged by size: 

                1028     341      17
 
NcoI C'CATG_G

Cuts at:      0      80     212     590    1386 
   Size:         80     132     378     796

  Fragments arranged by size: 

                 796     378     132      80
 
NgoMIV G'CCGG_C

Cuts at:      0    1266    1386 
   Size:       1266     120
 
NspI r_CATG'y

Cuts at:      0     690    1080    1386 
   Size:        690     390     306

  Fragments arranged by size: 

                 690     390     306
 
PasI CC'CwG_GG

Cuts at:      0     108     252     330    1386 
   Size:        108     144      78    1056

  Fragments arranged by size: 

                1056     144     108      78
 
PfoI T'CCnGG_A

Cuts at:      0     704    1386 
   Size:        704     682
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     760     792    1386 
   Size:        760      32     594

  Fragments arranged by size: 

                 760     594      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     354     495    1386 
   Size:        354     141     891

  Fragments arranged by size: 

                 891     354     141
 
PspOMI G'GGCC_C

Cuts at:      0     183     216    1386 
   Size:        183      33    1170

  Fragments arranged by size: 

                1170     183      33
 
PvuII CAG'CTG

Cuts at:      0     138     452    1386 
   Size:        138     314     934

  Fragments arranged by size: 

                 934     314     138
 
SacI G_AGCT'C

Cuts at:      0     166    1386 
   Size:        166    1220
 
SalI G'TCGA_C

Cuts at:      0     663    1386 
   Size:        663     723
 
ScaI AGT'ACT

Cuts at:      0     565    1386 
   Size:        565     821
 
SexAI A'CCwGG_T

Cuts at:      0     887    1386 
   Size:        887     499
 
SfoI GGC'GCC

Cuts at:      0    1029    1370    1386 
   Size:       1029     341      16

  Fragments arranged by size: 

                1029     341      16
 
SmaI CCC'GGG

Cuts at:      0     131    1386 
   Size:        131    1255
 
SmlI C'TyrA_G

Cuts at:      0     349    1386 
   Size:        349    1037
 
StuI AGG'CCT

Cuts at:      0    1204    1386 
   Size:       1204     182
 
StyI C'CwwG_G

Cuts at:      0      80     212     590    1139    1196    1386 
   Size:         80     132     378     549      57     190

  Fragments arranged by size: 

                 549     378     190     132      80      57
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      52     186     261     751    1350    1386 
   Size:         52     134      75     490     599      36

  Fragments arranged by size: 

                 599     490     134      75      52      36
 
TatI w'GTAC_w

Cuts at:      0     446     563    1386 
   Size:        446     117     823

  Fragments arranged by size: 

                 823     446     117
 
Tth111I GACn'n_nGTC

Cuts at:      0     790     889    1386 
   Size:        790      99     497

  Fragments arranged by size: 

                 790     497      99
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     177     249    1386 
   Size:        177      72    1137

  Fragments arranged by size: 

                1137     177      72
 
XmaI C'CCGG_G

Cuts at:      0     129    1386 
   Size:        129    1257
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AgeI     AleI     ApaI     AvaI    BamHI
     BanI    BanII     BbeI    BbvCI     BglI     BlpI Bme1580I    BmgBI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BstYI   Bsu36I     BtgI    BtgZI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
     FalI     FspI    HaeII    Hin4I   HincII     KasI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PfoI
     PpiI    PpuMI   PspOMI    PvuII     SacI     SalI     ScaI    SexAI
     SfoI     SmaI     SmlI     StuI     StyI    TaqII     TatI  Tth111I
     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI     AlfI
     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BbsI     BcgI    BciVI     BclI    BfrBI    BglII     BmrI
     BmtI     BplI    BsaBI    BsiWI    BsmBI    BspEI    BspHI    BsrDI
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtsI     ClaI
    CspCI     DraI   DraIII    EcoNI    EcoRI    EcoRV     FseI    FspAI
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII    SacII
    SanDI     SapI     SbfI     SfcI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TstI     XbaI     XhoI
     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com