Restriction map for pF1KB9769
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AflII       AgeI        AhdI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfrI        BglI        
BsaAI       BsaHI       BsaI        BseAI       BsiEI       
BsiWI       BsmBI       BspEI       BspLU11I    BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstEII      
BstPI       BstUI       BstZ17I     Cfr10I      DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     FseI        FspI        
HaeII       Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MluI        
MroI        MspA1I      MvnI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshBI       PvuI        PvuII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       Sse8387I    
StuI        SwaI        Tth111I     Van91I      XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI         2578  GTCTAC               GT^[AC][GT]AC
AflIII        971  ACGTGT               A^C[GA][CT]GT
Alw44I       3070  GTGCAC               G^TGCAC
ApaLI        3070  GTGCAC               G^TGCAC
AvrII        1041  CCTAGG               C^CTAGG
BanI         1818  GGCACC               G^G[CT][GA]CC
BcnI         1822  CCCGG                CC^[GC]GG
BlnI         1041  CCTAGG               C^CTAGG
BlpI         2293  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2293  GCTGAGC              GC^T[ACGT]AGC
BsaBI        1584  GATCTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1967  ACCGGA               [AT]^CCGG[AT]
BspDI        1591  ATCGAT               AT^CGAT
BspMI        2440  ACCTGC               ACCTGC(4/8)
BsrBI        2536  GAGCGG               CCGCTC(-3/-3)
BstBI        2749  TTCGAA               TT^CGAA
CelII        2293  GCTGAGC              GC^T[ACGT]AGC
CfoI         2467  GCGC                 GCG^C
ClaI         1591  ATCGAT               AT^CGAT
CpoI         2999  CGGACCG              CG^G[AT]CCG
DraI         2338  TTTAAA               TTT^AAA
DraII        1343  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO109I     1343  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRV         487  GATATC               GAT^ATC
EcoT22I      2269  ATGCAT               ATGCA^T
FauI         2538  GCGGG                CCCGC(4/6)
HgaI           22  GACGC                GACGC(5/10)
HhaI         2467  GCGC                 GCG^C
HinP1I       2467  GCGC                 G^CGC
MamI         1584  GATCTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI           57  CAATTG               C^AATTG
MunI           57  CAATTG               C^AATTG
NciI         1822  CCCGG                CC^[GC]GG
NcoI          821  CCATGG               C^CATGG
NsiI         2269  ATGCAT               ATGCA^T
NspI         2115  GCATGC               [GA]CATG^[CT]
NspV         2749  TTCGAA               TT^CGAA
PaeR7I       1901  CTCGAG               C^TCGAG
PpuMI        1343  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI        1776  GACTTTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1406  CTGCAG               CTGCA^G
RsrII        2999  CGGACCG              CG^G[AT]CCG
SfuI         2749  TTCGAA               TT^CGAA
SpeI         2458  ACTAGT               A^CTAGT
SphI         2115  GCATGC               GCATG^C
XcmI         2127  CCAGCCCTGTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1901  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AclI           88  AACGTT               AA^CGTT
             1889  AACGTT              
AlwI          877  GATCC                GGATC(4/5)
             2526  GATCC               
AlwNI        1241  CAGCTACTG            CAG[ACGT][ACGT][ACGT]^CTG
             2816  CAGAGTCTG           
Asp700I      1355  GAAGAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1857  GAATGCTTTC          
AvaI         1364  CTCGGG               C^[CT]CG[GA]G
             1901  CTCGAG              
BalI          227  TGGCCA               TGG^CCA
             2488  TGGCCA              
BbsI         2718  GAAGAC               GAAGAC(2/6)
              577  GTCTTC              
BclI         1955  TGATCA               T^GATCA
             2609  TGATCA              
BglII        1583  AGATCT               A^GATCT
             1989  AGATCT              
BpuAI        2718  GAAGAC               GAAGAC(2/6)
              577  GTCTTC              
BsmFI        2405  GGGAC                GGGAC(10/14)
             2694  GTCCC               
BsoBI        1364  CTCGGG               C^[CT]CG[GA]G
             1901  CTCGAG              
Bsp1407I       50  TGTACA               T^GTACA
             2099  TGTACA              
BspHI         437  TCATGA               T^CATGA
             2714  TCATGA              
BsrGI          50  TGTACA               T^GTACA
             2099  TGTACA              
BstXI        2046  CCATGTCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2243  CCACGGACTTGG        
BstYI        1583  AGATCT               [GA]^GATC[CT]
             1989  AGATCT              
EaeI          227  TGGCCA               [CT]^GGCC[GA]
             2488  TGGCCA              
EcoRI        1063  GAATTC               G^AATTC
             2658  GAATTC              
EcoT14I       821  CCATGG               C^C[AT][AT]GG
             1041  CCTAGG              
FbaI         1955  TGATCA               T^GATCA
             2609  TGATCA              
MflI         1583  AGATCT               [GA]^GATC[CT]
             1989  AGATCT              
MluNI         227  TGGCCA               TGG^CCA
             2488  TGGCCA              
MscI          227  TGGCCA               TGG^CCA
             2488  TGGCCA              
NdeI          793  CATATG               CA^TATG
             2266  CATATG              
Psp1406I       88  AACGTT               AA^CGTT
             1889  AACGTT              
RcaI          437  TCATGA               T^CATGA
             2714  TCATGA              
SacI          535  GAGCTC               GAGCT^C
             2423  GAGCTC              
SapI          441  GAAGAGC              GCTCTTC(1/4)
             1421  GAAGAGC             
SspBI          50  TGTACA               T^GTACA
             2099  TGTACA              
StyI          821  CCATGG               C^C[AT][AT]GG
             1041  CCTAGG              
XhoII        1583  AGATCT               [GA]^GATC[CT]
             1989  AGATCT              
XmnI         1355  GAAGAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1857  GAATGCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AocI        
ApoI        AspHI       AvaII       BanII       BbvI        
BfaI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmI        Bsp1286I    BsrDI       BsrI        BssKI       
BstF5I      BstNI       Bsu36I      Cac8I       Cfr13I      
DdeI        DpnII       DsaI        EarI        Eco81I      
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     
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