Restriction map for pF1KB9767
 (Linear) (Six-base) MAPSORT of: KIBB9767.seq  Check: 6330  from: 1  to: 2841



KIBB9767.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AccI GT'mk_AC

Cuts at:      0      15    2841 
   Size:         15    2826
 
Acc65I G'GTAC_C

Cuts at:      0    2782    2841 
   Size:       2782      59
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     257     569     650    1078    1241    1261    1265 
   Size:         79     178     312      81     428     163      20       4

Cuts at:   1265    1523    1961    2111    2841 
   Size:        258     438     150     730

  Fragments arranged by size: 

                 730     438     428     312     258     178     163     150
                  81      79      20       4
 
AfeI AGC'GCT

Cuts at:      0     244    2841 
   Size:        244    2597
 
AflII C'TTAA_G

Cuts at:      0    1968    2841 
   Size:       1968     873
 
AflIII A'CryG_T

Cuts at:      0    2181    2841 
   Size:       2181     660
 
AleI CACnn'nnGTG

Cuts at:      0     827    2841 
   Size:        827    2014
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1948    2841 
   Size:       1948     893
 
ApoI r'AATT_y

Cuts at:      0     527     688    1006    1839    2270    2841 
   Size:        527     161     318     833     431     571

  Fragments arranged by size: 

                 833     571     527     431     318     161
 
AseI AT'TA_AT

Cuts at:      0    1027    2841 
   Size:       1027    1814
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      82     115    2841 
   Size:         82      33    2726

  Fragments arranged by size: 

                2726      82      33
 
BanI G'GyrC_C

Cuts at:      0    2782    2841 
   Size:       2782      59
 
BanII G_rGCy'C

Cuts at:      0    2474    2841 
   Size:       2474     367
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     293     799    2841 
   Size:        293     506    2042

  Fragments arranged by size: 

                2042     506     293
 
BfrBI ATG'CAT

Cuts at:      0     874    1000    2081    2325    2841 
   Size:        874     126    1081     244     516

  Fragments arranged by size: 

                1081     874     516     244     126
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     353     579    1043    1294    1598    2675    2841 
   Size:        353     226     464     251     304    1077     166

  Fragments arranged by size: 

                1077     464     353     304     251     226     166
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2564    2678    2841 
   Size:       2564     114     163

  Fragments arranged by size: 

                2564     163     114
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1725    2841 
   Size:       1725    1116
 
BsaHI Gr'CG_yC

Cuts at:      0    2105    2841 
   Size:       2105     736
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     572     602     639     669    2841 
   Size:        572      30      37      30    2172

  Fragments arranged by size: 

                2172     572      37      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      32    1206    2772    2775    2778    2841 
   Size:         32    1174    1566       3       3      63

  Fragments arranged by size: 

                1566    1174      63      32       3       3
 
BseYI C'CCAG_C

Cuts at:      0     493    2841 
   Size:        493    2348
 
BsiHKAI G_wGCw'C

Cuts at:      0    2474    2653    2841 
   Size:       2474     179     188

  Fragments arranged by size: 

                2474     188     179
 
BsmI GAATG_Cn'

Cuts at:      0    1094    2083    2409    2841 
   Size:       1094     989     326     432

  Fragments arranged by size: 

                1094     989     432     326
 
Bsp1286I G_dGCh'C

Cuts at:      0    2474    2653    2841 
   Size:       2474     179     188

  Fragments arranged by size: 

                2474     188     179
 
BspHI T'CATG_A

Cuts at:      0    1034    2841 
   Size:       1034    1807
 
BsrDI GCAATG_nn'

Cuts at:      0    1407    2780    2841 
   Size:       1407    1373      61

  Fragments arranged by size: 

                1407    1373      61
 
BsrGI T'GTAC_A

Cuts at:      0    1296    2379    2841 
   Size:       1296    1083     462

  Fragments arranged by size: 

                1296    1083     462
 
BstBI TT'CG_AA

Cuts at:      0    1106    2841 
   Size:       1106    1735
 
BstYI r'GATC_y

Cuts at:      0     951    1124    2595    2841 
   Size:        951     173    1471     246

  Fragments arranged by size: 

                1471     951     246     173
 
Bsu36I CC'TnA_GG

Cuts at:      0    2283    2841 
   Size:       2283     558
 
BtsI GCAGTG_nn'

Cuts at:      0     721    1968    1992    2841 
   Size:        721    1247      24     849

  Fragments arranged by size: 

                1247     849     721      24
 
DraI TTT'AAA

Cuts at:      0     227    2841 
   Size:        227    2614
 
EarI CTCTTCn'nnn_

Cuts at:      0     401     628    1236    1980    2479    2654    2841 
   Size:        401     227     608     744     499     175     187

  Fragments arranged by size: 

                 744     608     499     401     227     187     175
 
EcoICRI GAG'CTC

Cuts at:      0    2472    2841 
   Size:       2472     369
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     257     353     569     579     650    1043    1078 
   Size:         79     178      96     216      10      71     393      35

Cuts at:   1078    1241    1261    1265    1294    1523    1598    1961    2111 
   Size:        163      20       4      29     229      75     363     150

Cuts at:   2111    2675    2841 
   Size:        564     166

  Fragments arranged by size: 

                 564     393     363     229     216     178     166     163
                 150      96      79      75      71      35      29      20
                  10       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2282    2841 
   Size:       2282     559
 
EcoRV GAT'ATC

Cuts at:      0    1176    2841 
   Size:       1176    1665
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     261     865    1640    1795    2841 
   Size:        261     604     775     155    1046

  Fragments arranged by size: 

                1046     775     604     261     155
 
HaeII r_GCGC'y

Cuts at:      0     246    2841 
   Size:        246    2595
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     572     604    1957    1981    1989    2013    2125    2157 
   Size:        572      32    1353      24       8      24     112      32

Cuts at:   2157    2841 
   Size:        684

  Fragments arranged by size: 

                1353     684     572     112      32      32      24      24
                   8
 
HincII GTy'rAC

Cuts at:      0      16      36     591     897    2841 
   Size:         16      20     555     306    1944

  Fragments arranged by size: 

                1944     555     306      20      16
 
HindIII A'AGCT_T

Cuts at:      0    1273    2841 
   Size:       1273    1568
 
HpaI GTT'AAC

Cuts at:      0      36    2841 
   Size:         36    2805
 
KpnI G_GTAC'C

Cuts at:      0    2786    2841 
   Size:       2786      55
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     721    2841 
   Size:        721    2120
 
MslI CAynn'nnrTG

Cuts at:      0     827     873    2317    2350    2791    2841 
   Size:        827      46    1444      33     441      50

  Fragments arranged by size: 

                1444     827     441      50      46      33
 
NdeI CA'TA_TG

Cuts at:      0     870    2841 
   Size:        870    1971
 
NsiI A_TGCA'T

Cuts at:      0     876    1002    2083    2327    2841 
   Size:        876     126    1081     244     514

  Fragments arranged by size: 

                1081     876     514     244     126
 
NspI r_CATG'y

Cuts at:      0    1721    2185    2841 
   Size:       1721     464     656

  Fragments arranged by size: 

                1721     656     464
 
PciI A'CATG_T

Cuts at:      0    2181    2841 
   Size:       2181     660
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2711    2841 
   Size:       2711     130
 
PsiI TTA'TAA

Cuts at:      0     208     887    2841 
   Size:        208     679    1954

  Fragments arranged by size: 

                1954     679     208
 
SacI G_AGCT'C

Cuts at:      0    2474    2841 
   Size:       2474     367
 
SalI G'TCGA_C

Cuts at:      0      14    2841 
   Size:         14    2827
 
SapI GCTCTTCn'nnn_

Cuts at:      0     401    1980    2479    2654    2841 
   Size:        401    1579     499     175     187

  Fragments arranged by size: 

                1579     499     401     187     175
 
SfcI C'TryA_G

Cuts at:      0     825    2615    2841 
   Size:        825    1790     226

  Fragments arranged by size: 

                1790     825     226
 
SmlI C'TyrA_G

Cuts at:      0    1968    2579    2693    2841 
   Size:       1968     611     114     148

  Fragments arranged by size: 

                1968     611     148     114
 
SspI AAT'ATT

Cuts at:      0     259    2841 
   Size:        259    2582
 
SwaI ATTT'AAAT

Cuts at:      0     227    2841 
   Size:        227    2614
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     409     507    2841 
   Size:        409      98    2334

  Fragments arranged by size: 

                2334     409      98
 
TatI w'GTAC_w

Cuts at:      0    1049    1296    2379    2841 
   Size:       1049     247    1083     462

  Fragments arranged by size: 

                1083    1049     462     247
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2111    2143    2841 
   Size:       2111      32     698

  Fragments arranged by size: 

                2111     698      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     934    2841 
   Size:        934    1907
 
XbaI T'CTAG_A

Cuts at:      0     363    1843    2841 
   Size:        363    1480     998

  Fragments arranged by size: 

                1480     998     363
 
XmnI GAAnn'nnTTC

Cuts at:      0    1778    2841 
   Size:       1778    1063
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI    AflII   AflIII     AleI    AlwNI
     ApoI     AseI     BaeI     BanI    BanII     BbsI    BfrBI     BpmI
    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BsrDI    BsrGI    BstBI    BstYI   Bsu36I     BtsI
     DraI     EarI  EcoICRI  Eco57MI    EcoNI    EcoRV     FalI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MmeI     MslI     NdeI
     NsiI     NspI     PciI    PflMI     PsiI     SacI     SalI     SapI
     SfcI     SmlI     SspI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AgeI     AhdI     AlfI     AloI     ApaI
    ApaLI     AscI    AsiSI     AvaI    AvrII    BamHI     BbeI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaWI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EciI EcoO109I    EcoRI
     FseI     FspI    FspAI     KasI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     XcmI     XhoI
     XmaI     ZraI
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