Restriction map for
pF1KB9762
(Circular) (Six-base) MAPSORT of: pF1KB9762.seq Check: 6810 from: 1 to: 5513
pF1KB9762 5513 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AatII G_ACGT'C
Cuts at: 2075 2075
Size: 5513
AccI GT'mk_AC
Cuts at: 863 1745 2551 863
Size: 882 806 3825
Fragments arranged by size:
3825 882 806
Acc65I G'GTAC_C
Cuts at: 1257 2529 1257
Size: 1272 4241
AclI AA'CG_TT
Cuts at: 257 5353 257
Size: 5096 417
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1054 1743 1775 3338 3770 4517 4785 1054
Size: 689 32 1563 432 747 268 1782
Fragments arranged by size:
1782 1563 747 689 432 268 32
AflIII A'CryG_T
Cuts at: 845 2865 3969 4946 845
Size: 2020 1104 977 1412
Fragments arranged by size:
2020 1412 1104 977
AgeI A'CCGG_T
Cuts at: 3901 3901
Size: 5513
AleI CACnn'nnGTG
Cuts at: 1531 1531
Size: 5513
AlwNI CAG_nnn'CTG
Cuts at: 649 2374 2818 4385 649
Size: 1725 444 1567 1777
Fragments arranged by size:
1777 1725 1567 444
ApaLI G'TGCA_C
Cuts at: 841 4283 841
Size: 3442 2071
ApoI r'AATT_y
Cuts at: 1018 2019 2279 2385 2397 2517 4866 5122 1018
Size: 1001 260 106 12 120 2349 256 1409
Fragments arranged by size:
2349 1409 1001 260 256 120 106 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 5513
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5513
AvaI C'yCGr_G
Cuts at: 504 2533 504
Size: 2029 3484
BamHI G'GATC_C
Cuts at: 1339 2538 1339
Size: 1199 4314
BanI G'GyrC_C
Cuts at: 348 1257 2529 2943 3191 3226 348
Size: 909 1272 414 248 35 2635
Fragments arranged by size:
2635 1272 909 414 248 35
BanII G_rGCy'C
Cuts at: 496 504 2044 2527 3557 496
Size: 8 1540 483 1030 2452
Fragments arranged by size:
2452 1540 1030 483 8
BbeI G_GCGC'C
Cuts at: 2947 3195 2947
Size: 248 5265
BbsI GAAGACnn'nnnn_
Cuts at: 692 692
Size: 5513
BbvCI CC'TCA_GC
Cuts at: 530 530
Size: 5513
BciVI GTATCCnnnnn_n'
Cuts at: 3411 4172 5513 3411
Size: 761 1341 3411
Fragments arranged by size:
3411 1341 761
BclI T'GATC_A
Cuts at: 3033 3033
Size: 5513
BglI GCCn_nnn'nGGC
Cuts at: 112 242 448 112
Size: 130 206 5177
Fragments arranged by size:
5177 206 130
BglII A'GATC_T
Cuts at: 1641 3028 5065 1641
Size: 1387 2037 2089
Fragments arranged by size:
2089 2037 1387
BlpI GC'TnA_GC
Cuts at: 2625 2625
Size: 5513
Bme1580I G_kGCm'C
Cuts at: 353 604 845 3138 3231 4287 353
Size: 251 241 2293 93 1056 1579
Fragments arranged by size:
2293 1579 1056 251 241 93
BmrI ACTGGGnnnn_n'
Cuts at: 2384 2725 2806 3141 5285 2384
Size: 341 81 335 2144 2612
Fragments arranged by size:
2612 2144 341 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3880 3880
Size: 5513
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 1586 496
Size: 1090 4423
Bpu10I CC'TnA_GC
Cuts at: 530 530
Size: 5513
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2689 3568 4060 4358 2689
Size: 879 492 298 3844
Fragments arranged by size:
3844 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 985 1528 2344 5217 985
Size: 543 816 2873 1281
Fragments arranged by size:
2873 1281 816 543
BsaAI yAC'GTr
Cuts at: 2866 2866
Size: 5513
BsaBI GATnn'nnATC
Cuts at: 6 3027 5064 6
Size: 3021 2037 455
Fragments arranged by size:
3021 2037 455
BsaHI Gr'CG_yC
Cuts at: 265 368 2072 2944 3192 3894 5390 265
Size: 103 1704 872 248 702 1496 388
Fragments arranged by size:
1704 1496 872 702 388 248 103
BsaWI w'CCGG_w
Cuts at: 363 496 1764 2702 3223 3901 4175 4322 4733
Size: 133 1268 938 521 678 274 147 411
Cuts at: 4733 363
Size: 1143
Fragments arranged by size:
1268 1143 938 678 521 411 274 147
133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2124 2154 2398 2428 2124
Size: 30 244 30 5209
Fragments arranged by size:
5209 244 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 493 139
Size: 354 5159
BseYI C'CCAG_C
Cuts at: 2938 3696 4273 4769 2938
Size: 758 577 496 3682
Fragments arranged by size:
3682 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 626 3055 3101 4309 104
Size: 522 2429 46 1208 1308
Fragments arranged by size:
2429 1308 1208 522 46
BsiHKAI G_wGCw'C
Cuts at: 845 1115 2527 3305 3495 4287 845
Size: 270 1412 778 190 792 2071
Fragments arranged by size:
2071 1412 792 778 270 190
BsmBI CGTCTCn'nnnn_
Cuts at: 633 633
Size: 5513
Bsp1286I G_dGCh'C
Cuts at: 353 496 504 604 845 1115 2044 2527 3138
Size: 143 8 100 241 270 929 483 611
Cuts at: 3138 3231 3305 3495 3557 4287 353
Size: 93 74 190 62 730 1579
Fragments arranged by size:
1579 929 730 611 483 270 241 190
143 100 93 74 62 8
BspEI T'CCGG_A
Cuts at: 363 496 1764 4733 363
Size: 133 1268 2969 1143
Fragments arranged by size:
2969 1268 1143 133
BspHI T'CATG_A
Cuts at: 4689 4689
Size: 5513
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2563 2777 3079 3460 2563
Size: 214 302 381 4616
Fragments arranged by size:
4616 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3805 3859 5343 5508 50
Size: 3755 54 1484 165 55
Fragments arranged by size:
3755 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3425 3425
Size: 5513
BsrFI r'CCGG_y
Cuts at: 370 523 1978 3511 3692 3901 370
Size: 153 1455 1533 181 209 1982
Fragments arranged by size:
1982 1533 1455 209 181 153
BsrGI T'GTAC_A
Cuts at: 2448 2448
Size: 5513
BssHII G'CGCG_C
Cuts at: 542 542
Size: 5513
BssSI C'ACGA_G
Cuts at: 3784 4142 3784
Size: 358 5155
BstAPI GCAn_nnn'nTGC
Cuts at: 2890 2890
Size: 5513
BstBI TT'CG_AA
Cuts at: 1008 3874 1008
Size: 2866 2647
BstEII G'GTnAC_C
Cuts at: 534 558 534
Size: 24 5489
BstYI r'GATC_y
Cuts at: 1 735 1339 1359 1641 2096 2538 3005 3028
Size: 734 604 20 282 455 442 467 23
Cuts at: 3028 3363 3609 4621 4707 4719 5065 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 734 604 467 455 449 442 346
335 282 246 86 23 20 12
Bsu36I CC'TnA_GG
Cuts at: 158 642 158
Size: 484 5029
BtgI C'CryG_G
Cuts at: 107 305 620 909 107
Size: 198 315 289 4711
Fragments arranged by size:
4711 315 289 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1183 3463 3644 3807 1183
Size: 2280 181 163 2889
Fragments arranged by size:
2889 2280 181 163
BtsI GCAGTG_nn'
Cuts at: 491 1526 1538 2756 491
Size: 1035 12 1218 3248
Fragments arranged by size:
3248 1218 1035 12
DraI TTT'AAA
Cuts at: 2512 2512
Size: 5513
DrdI GACnn_nn'nnGTC
Cuts at: 3219 4077 3219
Size: 858 4655
EaeI y'GGCC_r
Cuts at: 401 1980 3098 3272 3663 3690 5035 401
Size: 1579 1118 174 391 27 1345 879
Fragments arranged by size:
1579 1345 1118 879 391 174 27
EagI C'GGCC_G
Cuts at: 3098 3098
Size: 5513
EarI CTCTTCn'nnn_
Cuts at: 440 1054 1274 1397 1794 3536 3746 440
Size: 614 220 123 397 1742 210 2207
Fragments arranged by size:
2207 1742 614 397 220 210 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 136 309 3721 4031 4177 5323 136
Size: 173 3412 310 146 1146 326
Fragments arranged by size:
3412 1146 326 310 173 146
EcoICRI GAG'CTC
Cuts at: 2525 2525
Size: 5513
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 1054 1586 1743 1775 3338 3770 4517 4785
Size: 558 532 157 32 1563 432 747 268
Cuts at: 4785 496
Size: 1224
Fragments arranged by size:
1563 1224 747 558 532 432 268 157
32
EcoO109I rG'GnC_Cy
Cuts at: 293 646 2652 293
Size: 353 2006 3154
Fragments arranged by size:
3154 2006 353
EcoRI G'AATT_C
Cuts at: 2397 2517 2397
Size: 120 5393
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 912 3532 912
Size: 2620 2893
FspI TGC'GCA
Cuts at: 3294 3294
Size: 5513
HaeII r_GCGC'y
Cuts at: 118 377 454 524 530 611 2947 3195 4217
Size: 259 77 70 6 81 2336 248 1022
Cuts at: 4217 5196 118
Size: 979 435
Fragments arranged by size:
2336 1022 979 435 259 248 81 77
70 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1648 1680 2124 2156 3355 3385 3387 3417 1648
Size: 32 444 32 1199 30 2 30 3744
Fragments arranged by size:
3744 1199 444 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2552 2552
Size: 5513
HindIII A'AGCT_T
Cuts at: 1615 1615
Size: 5513
KasI G'GCGC_C
Cuts at: 2943 3191 2943
Size: 248 5265
KpnI G_GTAC'C
Cuts at: 1261 2533 1261
Size: 1272 4241
MluI A'CGCG_T
Cuts at: 4946 4946
Size: 5513
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 648 980 1400 2199 4184 4368 648
Size: 332 420 799 1985 184 1793
Fragments arranged by size:
1985 1793 799 420 332 184
MscI TGG'CCA
Cuts at: 3274 3274
Size: 5513
MslI CAynn'nnrTG
Cuts at: 878 1531 3502 3629 878
Size: 653 1971 127 2762
Fragments arranged by size:
2762 1971 653 127
MspA1I CmG'CkG
Cuts at: 325 358 2624 2938 3298 4311 4556 325
Size: 33 2266 314 360 1013 245 1282
Fragments arranged by size:
2266 1282 1013 360 314 245 33
NaeI GCC'GGC
Cuts at: 372 525 1980 3694 372
Size: 153 1455 1714 2191
Fragments arranged by size:
2191 1714 1455 153
NarI GG'CG_CC
Cuts at: 2944 3192 2944
Size: 248 5265
NcoI C'CATG_G
Cuts at: 107 909 107
Size: 802 4711
NdeI CA'TA_TG
Cuts at: 1893 1893
Size: 5513
NgoMIV G'CCGG_C
Cuts at: 370 523 1978 3692 370
Size: 153 1455 1714 2191
Fragments arranged by size:
2191 1714 1455 153
NspI r_CATG'y
Cuts at: 849 2566 3066 3973 849
Size: 1717 500 907 2389
Fragments arranged by size:
2389 1717 907 500
PciI A'CATG_T
Cuts at: 845 3969 845
Size: 3124 2389
PmeI GTTT'AAAC
Cuts at: 2512 2512
Size: 5513
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4652 4684 4652
Size: 32 5481
PpuMI rG'GwC_Cy
Cuts at: 293 646 293
Size: 353 5160
PstI C_TGCA'G
Cuts at: 413 1533 2049 2490 2560 3245 413
Size: 1120 516 441 70 685 2681
Fragments arranged by size:
2681 1120 685 516 441 70
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5513
PvuII CAG'CTG
Cuts at: 2938 3298 2938
Size: 360 5153
RsrII CG'GwC_CG
Cuts at: 3708 3708
Size: 5513
SacI G_AGCT'C
Cuts at: 2527 2527
Size: 5513
SalI G'TCGA_C
Cuts at: 2550 2550
Size: 5513
SanDI GG'GwC_CC
Cuts at: 293 293
Size: 5513
SapI GCTCTTCn'nnn_
Cuts at: 3536 3746 3536
Size: 210 5303
SbfI CC_TGCA'GG
Cuts at: 2560 2560
Size: 5513
ScaI AGT'ACT
Cuts at: 5030 5030
Size: 5513
SexAI A'CCwGG_T
Cuts at: 2474 2474
Size: 5513
SfcI C'TryA_G
Cuts at: 33 409 759 1529 1946 2045 2162 2366 2486
Size: 376 350 770 417 99 117 204 120
Cuts at: 2486 2556 3241 4234 4425 33
Size: 70 685 993 191 1121
Fragments arranged by size:
1121 993 770 685 417 376 350 204
191 120 117 99 70
SfoI GGC'GCC
Cuts at: 2945 3193 2945
Size: 248 5265
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5513
SgrAI Cr'CCGG_yG
Cuts at: 370 523 370
Size: 153 5360
SmaI CCC'GGG
Cuts at: 506 2535 506
Size: 2029 3484
SmlI C'TyrA_G
Cuts at: 2668 3583 4075 4337 2668
Size: 915 492 262 3844
Fragments arranged by size:
3844 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5513
SphI G_CATG'C
Cuts at: 2566 3066 2566
Size: 500 5013
SspI AAT'ATT
Cuts at: 1973 1973
Size: 5513
StyI C'CwwG_G
Cuts at: 107 909 1464 2647 3001 107
Size: 802 555 1183 354 2619
Fragments arranged by size:
2619 1183 802 555 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 401 1623 1940 2368 3096 3229 3688 3897 401
Size: 1222 317 428 728 133 459 209 2017
Fragments arranged by size:
2017 1222 728 459 428 317 209 133
TatI w'GTAC_w
Cuts at: 848 2448 5028 848
Size: 1600 2580 1333
Fragments arranged by size:
2580 1600 1333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5030 5062 5030
Size: 32 5481
Tth111I GACn'n_nGTC
Cuts at: 3310 3310
Size: 5513
XbaI T'CTAG_A
Cuts at: 2544 2544
Size: 5513
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 306 3008 114
Size: 192 2702 2619
Fragments arranged by size:
2702 2619 192
XmaI C'CCGG_G
Cuts at: 504 2533 504
Size: 2029 3484
XmnI GAAnn'nnTTC
Cuts at: 775 775
Size: 5513
ZraI GAC'GTC
Cuts at: 2073 2073
Size: 5513
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsgI BsiWI
BsmI BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SfiI SnaBI SrfI StuI SwaI
XhoI